{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:46:53Z","timestamp":1790923613079,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades from the Spanish government","doi-asserted-by":"publisher","award":["PID2019-110260RB-I00"],"award-info":[{"award-number":["PID2019-110260RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"crowdfunding initiative \u201cyomecorono.com\u201d","award":["PID2019-110260RB-I00"],"award-info":[{"award-number":["PID2019-110260RB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine epidemic diarrhea virus (PEDV) is characterized by diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. Two distinct genogroups, S-INDEL (G1a, G1b) and non-S INDEL (G2a, G2b, and G2c), circulate worldwide and are characterized by varying degrees of virulence. Here, we compared the early pathogenesis of a PEDV S-INDEL strain obtained from intestine homogenate (CALAF-HOMOG) or adapted to cell culture by 22 passages (CALAF-ADAP) and a virulent non-S INDEL strain (PEDV-USA) in newborn piglets. After orogastric inoculation of PEDV strains, body weight, temperature and clinical signs were monitored for 48 hpi. Pathological studies were performed at 48 hpi and RNA extracts from jejunal content (at 48 hpi) and rectal swabs (at 0 and 48 hpi) were tested for the presence of PEDV RNA as well as sequenced and compared to the inoculum. Piglets inoculated with PEDV-USA and CALAF-HOMOG isolates showed more severe weight loss, diarrhea, villi fusion and atrophy compared to CALAF-ADAP inoculated piglets. The viral load of rectal swabs was higher in the PEDV-USA inoculated group, followed by CALAF-HOMOG and CALAF-ADAP isolates. Similarly, viral RNA load in jejunal content was comparable among PEDV-USA and CALAF-HOMOG inoculated piglets and higher than that of CALAF-ADAP ones. The comparison of three full PEDV sequences of the inocula with the corresponding ones of pigs after 48 hpi yielded a nucleotide identity &gt;99.9%. This study highlights variations in virulence among S-INDEL and non-S INDEL strains and between S-INDEL isolates obtained from homogenate and cell culture.<\/jats:p>","DOI":"10.3390\/v16010044","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T07:45:32Z","timestamp":1703663132000},"page":"44","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinical, Pathological and Virological Outcomes of Tissue-Homogenate-Derived and Cell-Adapted Strains of Porcine Epidemic Diarrhea Virus (PEDV) in a Neonatal Pig Model"],"prefix":"10.3390","volume":"16","author":[{"given":"Carlos","family":"L\u00f3pez-Figueroa","sequence":"first","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esmeralda","family":"Cano","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Navarro","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"P\u00e9rez-Ma\u00edllo","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Pujols","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9890-7695","authenticated-orcid":false,"given":"Jos\u00e9 I.","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-444X","authenticated-orcid":false,"given":"J\u00falia","family":"Vergara-Alert","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1539-7261","authenticated-orcid":false,"given":"Joaquim","family":"Segal\u00e9s","sequence":"additional","affiliation":[{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, Barcelona, Spain"},{"name":"Departament de Sanitat i Anatomia Animals, Facultat de Veterin\u00e0ria, Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,27]]},"reference":[{"key":"ref_1","unstructured":"Saif, L.J., Wang, Q., Vlasova, A.N., Jung, K., and Xiao, S. 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