{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:34:35Z","timestamp":1789166075118,"version":"build-2803163510"},"reference-count":20,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Provincial Agricultural Major Science and Technology Program","award":["202202AE090027"],"award-info":[{"award-number":["202202AE090027"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine pseudorabies has long existed in China and is a serious threat to the Chinese farming industry. To understand the prevalence and genetic variation of the porcine pseudorabies virus (PRV) and its pathogenicity in Yunnan Province, China, we collected 560 serum samples across seven Yunnan Province regions from 2020 to 2021 and detected anti-gE antibodies in these samples. Sixty-one clinical tissue samples were also collected from pigs with suspected PRV that were vaccinated with Bartha-K61. PRV-gE antibodies were found in 29.6% (166\/560) of the serum samples. The PRV positivity rate in clinical tissue samples was 13.1% (8\/61). Two isolates, PRV-KM and PRV-QJ, were obtained. The identity of the gB, gD, and gE genes between these isolates and the Chinese mutants exceeded 99.5%. These isolates and the classical Fa strain were used to infect 4-week-old rats intranasally to assess their pathogenicity. All infected rats showed the typical clinical and pathological features of PRV two days post-infection. The viral loads in the organs differed significantly among the infected groups. Viruses were detected in the saliva and feces at 12 h. Significant dynamic changes in total white blood cell counts (WBC), lymphocyte counts (Lym), and neutrophil counts (Gran) occurred in the blood of the infected groups at 24 and 48 h. These results show that mutant PRV strains are prevalent in Bartha-K61-vaccinated pigs in Yunnan Province, China. Moreover, rats shed PRV in their saliva and feces during early infection, indicating the need for rodent control in combatting PRV infections in Yunnan Province, China.<\/jats:p>","DOI":"10.3390\/v16020233","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T11:25:55Z","timestamp":1706786755000},"page":"233","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation and Characterization of Yunnan Variants of the Pseudorabies Virus and Their Pathogenicity in Rats"],"prefix":"10.3390","volume":"16","author":[{"given":"Chunlian","family":"Song","sequence":"first","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yao","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Gao","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Weixin County Animal Husbandry Technology Extension Station, Zhaotong 657000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yougeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Animal Disease Prevention and Control Center of Weixin County, Zhaotong 657000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghua","family":"Shu","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular biology of pseudorabies virus: Impact on neurovirology and veterinary medicine","volume":"69","author":"Pomeranz","year":"2005","journal-title":"Microbiol. 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