{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T09:33:25Z","timestamp":1780652005890,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T00:00:00Z","timestamp":1708041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Program sponsored by the Ministry of Science and Technology (MOST)","award":["2023YFC2305900"],"award-info":[{"award-number":["2023YFC2305900"]}]},{"name":"Key Research and Development Program sponsored by the Ministry of Science and Technology (MOST)","award":["2022ACCP-MS06"],"award-info":[{"award-number":["2022ACCP-MS06"]}]},{"name":"Key Research and Development Program sponsored by the Ministry of Science and Technology (MOST)","award":["22TQ015"],"award-info":[{"award-number":["22TQ015"]}]},{"name":"Advanced Customer Cultivation Project of Wuhan National Biosafety Laboratory, Chinese Academy of Sciences","award":["2023YFC2305900"],"award-info":[{"award-number":["2023YFC2305900"]}]},{"name":"Advanced Customer Cultivation Project of Wuhan National Biosafety Laboratory, Chinese Academy of Sciences","award":["2022ACCP-MS06"],"award-info":[{"award-number":["2022ACCP-MS06"]}]},{"name":"Advanced Customer Cultivation Project of Wuhan National Biosafety Laboratory, Chinese Academy of Sciences","award":["22TQ015"],"award-info":[{"award-number":["22TQ015"]}]},{"name":"Shanghai Pilot Program for Basic Research, Fudan University","award":["2023YFC2305900"],"award-info":[{"award-number":["2023YFC2305900"]}]},{"name":"Shanghai Pilot Program for Basic Research, Fudan University","award":["2022ACCP-MS06"],"award-info":[{"award-number":["2022ACCP-MS06"]}]},{"name":"Shanghai Pilot Program for Basic Research, Fudan University","award":["22TQ015"],"award-info":[{"award-number":["22TQ015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Haemaphysalis longicornis (the longhorned tick), the predominant tick species in China, serves as a vector for a variety of pathogens, and is capable of transmitting the tick-borne encephalitis virus (TBEV), the causative agent of tick-borne encephalitis. However, it is unclear how these ticks transmit TBEV. Langat virus (LGTV), which has a reduced pathogenicity in humans, has been used as a surrogate for TBEV. In this study, we aimed to investigate the vector competence of H. longicornis to transmit LGTV and demonstrate the efficient acquisition and transmission of LGTV between this tick species and mice. LGTV localization was detected in several tick tissues, such as the midgut, salivary glands, and synganglion, using quantitative PCR and immunohistochemical staining with a polyclonal antibody targeting the LGTV envelope protein. We demonstrated the horizontal transmission of LGTV to different developmental stages within the same generation but did not see evidence of vertical transmission. It was interesting to note that we observed mice acting as a bridge, facilitating the transmission of LGTV to neighboring na\u00efve ticks during blood feeding. In conclusion, the virus\u2013vector\u2013host model employed in this study provides valuable insights into the replication and transmission of LGTV throughout its life cycle.<\/jats:p>","DOI":"10.3390\/v16020304","type":"journal-article","created":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T06:00:25Z","timestamp":1708063225000},"page":"304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Vector Competence of Asian Longhorned Ticks in Langat Virus Transmission"],"prefix":"10.3390","volume":"16","author":[{"given":"Yan","family":"Xu","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6794-2604","authenticated-orcid":false,"given":"Jingwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Raney, W.R., Herslebs, E.J., Langohr, I.M., Stone, M.C., and Hermance, M.E. 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