{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:20:28Z","timestamp":1762273228272,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Science and Technology Projects","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]},{"name":"Natural Science Foundation of China","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]},{"name":"Natural Science Foundation of China","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]},{"name":"Guangxi Basic Research Funds supported by the Special Project","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]},{"name":"Guangxi BaGui Scholars Program Foundation","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]},{"name":"Guangxi Science Base and Talents Special Program","award":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"],"award-info":[{"award-number":["AA23062050","31660715","32300148","Guike special 23-1","2019A50","AD17195083"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Interferon-inducible transmembrane protein 3 (IFITM3) is an antiviral factor that plays an important role in the host innate immune response against viruses. Previous studies have shown that IFITM3 is upregulated in various tissues and organs after avian reovirus (ARV) infection, which suggests that IFITM3 may be involved in the antiviral response after ARV infection. In this study, the chicken IFITM3 gene was cloned and analyzed bioinformatically. Then, the role of chicken IFITM3 in ARV infection was further explored. The results showed that the molecular weight of the chicken IFITM3 protein was approximately 13 kDa. This protein was found to be localized mainly in the cytoplasm, and its protein structure contained the CD225 domain. The homology analysis and phylogenetic tree analysis showed that the IFITM3 genes of different species exhibited great variation during genetic evolution, and chicken IFITM3 shared the highest homology with that of Anas platyrhynchos and displayed relatively low homology with those of birds such as Anser cygnoides and Serinus canaria. An analysis of the distribution of chicken IFITM3 in tissues and organs revealed that the IFITM3 gene was expressed at its highest level in the intestine and in large quantities in immune organs, such as the bursa of Fabricius, thymus and spleen. Further studies showed that the overexpression of IFITM3 in chicken embryo fibroblasts (DF-1) could inhibit the replication of ARV, whereas the inhibition of IFITM3 expression in DF-1 cells promoted ARV replication. In addition, chicken IFITM3 may exert negative feedback regulatory effects on the expression of TBK1, IFN-\u03b3 and IRF1 during ARV infection, and it is speculated that IFITM3 may participate in the innate immune response after ARV infection by negatively regulating the expression of TBK1, IFN-\u03b3 and IRF1. The results of this study further enrich the understanding of the role and function of chicken IFITM3 in ARV infection and provide a theoretical basis for an in-depth understanding of the antiviral mechanism of host resistance to ARV infection.<\/jats:p>","DOI":"10.3390\/v16030330","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T10:52:00Z","timestamp":1708512720000},"page":"330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of Chicken IFITM3 Gene Expression and Its Effect on Avian Reovirus Replication"],"prefix":"10.3390","volume":"16","author":[{"given":"Hongyu","family":"Ren","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530000, China"},{"name":"Key Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and 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Sci. Tech."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"638","DOI":"10.2307\/1593104","article-title":"The history of avian reovirus","volume":"44","year":"2000","journal-title":"Avian Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/s11262-013-1029-5","article-title":"Sequencing and phylogenetic analysis of an avian reovirus genome","volume":"48","author":"Teng","year":"2014","journal-title":"Virus Genes"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3550","DOI":"10.3382\/ps\/pey239","article-title":"Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil","volume":"97","author":"Souza","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.meegid.2016.01.018","article-title":"Whole genome alignment based one-step real-time RT-PCR for universal detection of avian orthoreoviruses of chicken, pheasant and turkey origins","volume":"39","author":"Tang","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"14727","DOI":"10.1038\/srep14727","article-title":"Isolation and molecular characterization of newly emerging avian reovirus variants and novel strains in Pennsylvania, USA, 2011\u20132014","volume":"5","author":"Lu","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/nri3665","article-title":"Innate immunity to influenza virus infection","volume":"14","author":"Iwasaki","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/j.jmb.2013.11.023","article-title":"Innate immunity to dengue virus infection and subversion of antiviral responses","volume":"426","author":"Green","year":"2014","journal-title":"J. Mol. Biol."},{"key":"ref_9","first-page":"73","article-title":"The interferon response circuit in antiviral host defense","volume":"71","author":"Haller","year":"2009","journal-title":"Verh.-K. Acad. Voor Geneeskd. Van Belg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern recognition receptors and inflammation","volume":"140","author":"Takeuchi","year":"2010","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3389\/fimmu.2017.00209","article-title":"Evolution of Interferons and Interferon Receptors","volume":"8","author":"Secombes","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1007\/s00705-019-04340-8","article-title":"Altered gene expression profiles of the MDA5 signaling pathway in peripheral blood lymphocytes of chickens infected with avian reovirus","volume":"164","author":"Xie","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"618124","DOI":"10.3389\/fvets.2021.618124","article-title":"Dynamic Changes in the Expression of Interferon-Stimulated Genes in Joints of SPF Chickens Infected With Avian Reovirus","volume":"8","author":"Wang","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, S., Wan, L., Ren, H., Xie, Z., Xie, L., Huang, J., Deng, X., Xie, Z., Luo, S., and Li, M. (2022). Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.998505"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/imcb.12000","article-title":"The anti-inflammatory IFITM genes ameliorate colitis and partially protect from tumorigenesis by changing immunity and microbiota","volume":"96","author":"Alteber","year":"2018","journal-title":"Immunol. Cell Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1016\/j.celrep.2015.09.048","article-title":"A Role for IFITM Proteins in Restriction of Mycobacterium tuberculosis Infection","volume":"13","author":"Ranjbar","year":"2015","journal-title":"Cell Rep."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Huang, I.C., Bailey, C.C., Weyer, J.L., Radoshitzky, S.R., Becker, M.M., Chiang, J.J., Brass, A.L., Ahmed, A.A., Chi, X., and Dong, L. (2011). Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1001258"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"17261","DOI":"10.1074\/jbc.M112.438515","article-title":"Interferon-inducible transmembrane protein 3 (IFITM3) restricts reovirus cell entry","volume":"288","author":"Anafu","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1128\/JVI.02113-07","article-title":"Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus","volume":"82","author":"Jiang","year":"2008","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, A., Sun, L., Wang, M., Jia, R., Zhu, D., Liu, M., Sun, K., Yang, Q., Wu, Y., and Chen, X. (2017). Identification of IFITM1 and IFITM3 in Goose: Gene Structure, Expression Patterns, and Immune Reponses against Tembusu Virus Infection. Biomed. Res. Int., 2017.","DOI":"10.1155\/2017\/5149062"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Feeley, E.M., Sims, J.S., John, S.P., Chin, C.R., Pertel, T., Chen, L.M., Gaiha, G.D., Ryan, B.J., Donis, R.O., and Elledge, S.J. (2011). IFITM3 inhibits influenza A virus infection by preventing cytosolic entry. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002337"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.cell.2009.12.017","article-title":"IFITM Proteins Mediate the Innate Immune Response to Influenza A H1N1 Virus, West Nile Virus and Dengue Virus","volume":"139","author":"Brass","year":"2009","journal-title":"Cell"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Pang, Z., Hao, P., Qu, Q., Li, L., Jiang, Y., Xiao, S., Jin, N., and Li, C. (2022). Interferon-Inducible Transmembrane Protein 3 (IFITM3) Restricts Rotavirus Infection. Viruses, 14.","DOI":"10.3390\/v14112407"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4937","DOI":"10.1016\/j.jmb.2013.09.024","article-title":"IFITMs restrict the replication of multiple pathogenic viruses","volume":"425","author":"Perreira","year":"2013","journal-title":"J. Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nri3344","article-title":"The broad-spectrum antiviral functions of IFIT and IFITM proteins","volume":"13","author":"Diamond","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.antiviral.2014.06.008","article-title":"Swine interferon-induced transmembrane protein, sIFITM3, inhibits foot-and-mouth disease virus infection in vitro and in vivo","volume":"109","author":"Xu","year":"2014","journal-title":"Antivir. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1089\/vim.2020.0132","article-title":"IFITMs of African Green Monkey Can Inhibit Replication of SFTSV but Not MNV In Vitro","volume":"33","author":"Xing","year":"2020","journal-title":"Viral Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s12250-015-3696-y","article-title":"Responses of the Toll-like receptor and melanoma differentiation-associated protein 5 signaling pathways to avian infectious bronchitis virus infection in chicks","volume":"31","author":"He","year":"2016","journal-title":"Virol. Sin."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7837","DOI":"10.1128\/JVI.00481-13","article-title":"The CD225 domain of IFITM3 is required for both IFITM protein association and inhibition of influenza A virus and dengue virus replication","volume":"87","author":"John","year":"2013","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.tig.2021.01.004","article-title":"CD225 Proteins: A Family Portrait of Fusion Regulators","volume":"37","author":"Coomer","year":"2021","journal-title":"Trends Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s11259-019-09762-y","article-title":"Genetic characteristics and polymorphisms in the chicken interferon-induced transmembrane protein (IFITM3) gene","volume":"43","author":"Kim","year":"2019","journal-title":"Vet. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e58537","DOI":"10.7554\/eLife.58537","article-title":"Homology-guided identification of a conserved motif linking the antiviral functions of IFITM3 to its oligomeric state","volume":"9","author":"Rahman","year":"2020","journal-title":"eLife"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"12957","DOI":"10.1128\/JVI.01443-13","article-title":"Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro","volume":"87","author":"Smith","year":"2013","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3858\/emm.2010.42.2.011","article-title":"Identification of the polymorphisms in IFITM3 gene and their association in a Korean population with ulcerative colitis","volume":"42","author":"Seo","year":"2010","journal-title":"Exp. Mol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.vetimm.2011.04.012","article-title":"Intestinal gene expression in pigs experimentally co-infected with PCV2 and PPV","volume":"142","author":"Andersson","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/03079458508436210","article-title":"Experimental reovirus infection in chickens: Observations on early viraemia and virus distribution in bone marrow, liver and enteric tissues","volume":"14","author":"Kibenge","year":"1985","journal-title":"Avian Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Wan, L., Wang, S., Xie, Z., Ren, H., Xie, L., Luo, S., Li, M., Xie, Z., Fan, Q., and Zeng, T. (2023). Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1237438"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1128\/JVI.01593-15","article-title":"Duck Interferon-Inducible Transmembrane Protein 3 Mediates Restriction of Influenza Viruses","volume":"90","author":"Blyth","year":"2016","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chen, S., Wang, L., Chen, J., Zhang, L., Wang, S., Goraya, M.U., Chi, X., Na, Y., Shao, W., and Yang, Z. (2017). Avian Interferon-Inducible Transmembrane Protein Family Effectively Restricts Avian Tembusu Virus Infection. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00672"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1038\/cmi.2017.15","article-title":"IFITM3 inhibits virus-triggered induction of type I interferon by mediating autophagosome-dependent degradation of IRF3","volume":"15","author":"Jiang","year":"2018","journal-title":"Cell. Mol. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.tibs.2008.01.002","article-title":"Are the IKKs and IKK-related kinases TBK1 and IKK-epsilon similarly activated?","volume":"33","author":"Chau","year":"2008","journal-title":"Trends Biochem. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1002\/eji.201040814","article-title":"Novel splice variants of human IKKepsilon negatively regulate IKKepsilon-induced IRF3 and NF-kB activation","volume":"41","author":"Koop","year":"2011","journal-title":"Eur. J. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.ejphar.2014.08.034","article-title":"Induction of interferon-induced transmembrane protein 3 gene expression by lipopolysaccharide in astrocytes","volume":"745","author":"Nakajima","year":"2014","journal-title":"Eur. J. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1292\/jvms.11-0517","article-title":"Biological effects of chicken type III interferon on expression of interferon-stimulated genes in chickens: Comparison with type I and type II interferons","volume":"74","author":"Masuda","year":"2012","journal-title":"J. Vet. Med. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1002\/jcb.10142","article-title":"Interferon regulatory factor-1 mediates interferon-gamma-induced apoptosis in ovarian carcinoma cells","volume":"85","author":"Kim","year":"2002","journal-title":"J. Cell. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1006\/bbrc.1999.0276","article-title":"IRF-1 is an essential mediator in IFN-gamma-induced cell cycle arrest and apoptosis of primary cultured hepatocytes","volume":"257","author":"Kano","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1189\/jlb.0603252","article-title":"Interferon-gamma: An overview of signals, mechanisms and functions","volume":"75","author":"Schroder","year":"2004","journal-title":"J. Leukoc. Biol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/3\/330\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:02:34Z","timestamp":1760104954000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/3\/330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,21]]},"references-count":47,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["v16030330"],"URL":"https:\/\/doi.org\/10.3390\/v16030330","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2024,2,21]]}}}