{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T07:31:46Z","timestamp":1780731106093,"version":"3.54.1"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T00:00:00Z","timestamp":1708732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian Federal Foundation","award":["88881.369357\/2019-01"],"award-info":[{"award-number":["88881.369357\/2019-01"]}]},{"DOI":"10.13039\/501100002322","name":"Evaluation of Graduate Education (Capes)","doi-asserted-by":"publisher","award":["88881.369357\/2019-01"],"award-info":[{"award-number":["88881.369357\/2019-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fern-Audette Endowment and University of Florida","award":["88881.369357\/2019-01"],"award-info":[{"award-number":["88881.369357\/2019-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>We investigated the interaction between the insect-specific virus, Piura virus (PIUV), and the arbovirus Zika virus (ZIKV) in Aedes albopictus cells. We performed coinfection experiments in C6\/36 cells. Piura virus (Cor 33 strain, Colombia) and ZIKV (PRVABC58 strain, Puerto Rico) were co-inoculated into C6\/36 cells using two multiplicity of infection (MOI) combinations: 0.1 for both viruses and 1.0 for ZIKV, 0.1 for PIUV. Wells were infected in triplicate with either PIUV and ZIKV coinfection, ZIKV-only, or PIUV-only. Mock infected cells served as control wells. The cell suspension was collected daily 7 days post-infection. Zika virus load was titrated by TCID50 on Vero 76 cells. The ZIKV-only infection and PIUV and ZIKV coinfection experiments were also quantified by RT-qPCR. We also investigated whether ZIKV interfered in the PIUV replication. PIUV suppressed the replication of ZIKV, resulting in a 10,000-fold reduction in ZIKV titers within 3 days post-infection. PIUV viral loads were not reduced in the presence of ZIKV. We conclude that, when concurrently infected, PIUV suppresses ZIKV in C6\/36 cells while ZIKV does not interfere in PIUV replication.<\/jats:p>","DOI":"10.3390\/v16030350","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T07:51:39Z","timestamp":1708933899000},"page":"350","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8385-8253","authenticated-orcid":false,"given":"Val\u00e9ria L.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Rodovia BR-316, Km 7, s\/n, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-5381","authenticated-orcid":false,"given":"Dhani","family":"Prakoso","sequence":"additional","affiliation":[{"name":"Professor Nidom Foundation, Surabaya, East Java 60236, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika R.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Montana Veterinary Diagnostic Laboratory, 1911 W Lincoln St., Bozeman, MT 59718, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4152-6936","authenticated-orcid":false,"given":"Tracey D.","family":"Logan","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, 1225 Center Dr. Suite 4101, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-9665","authenticated-orcid":false,"given":"Bruno Tardelli Diniz","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Rodovia BR-316, Km 7, s\/n, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Beachboard","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Ave., Gainesville, FL 32608, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5337-9024","authenticated-orcid":false,"given":"Maureen T.","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Ave., Gainesville, FL 32608, USA"},{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Road, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1089\/vbz.2016.1952","article-title":"Emergence of Human Arboviral Diseases in the Americas, 2000\u20132016","volume":"16","author":"Vasconcelos","year":"2016","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_2","first-page":"e41717","article-title":"Dengue, Chikungunya, and Zika: The Causes and Threats of Emerging and Re-emerging Arboviral Diseases","volume":"15","author":"Soni","year":"2023","journal-title":"Cureus"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Masmejan, S., Musso, D., Vouga, M., Pomar, L., Dashraath, P., Stojanov, M., Panchaud, A., and Baud, D. 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