{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:15:04Z","timestamp":1790356504353,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T00:00:00Z","timestamp":1711756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["CARS-42-19"],"award-info":[{"award-number":["CARS-42-19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Goose astrovirus genotype 1 (GAstV-1) has emerged in goose farms in some provinces of China in recent years and is considered to be one of the pathogens of gout in goslings in China. However, few studies have been conducted on the dynamic distribution, tissue tropism, and pathogenesis of GAstV-1 in goslings. In 2022, an epidemiological investigation of goose astrovirus (GAstV) in goslings was conducted in seven provinces of China. During the investigation, a GAstV-1 designated as GAstV-JSXZ was identified in the kidney of an 8-day-old gosling and was successfully isolated from a goose embryo. The full genome sequence of GAstV-JSXZ was determined using the next-generation sequencing technique. The complete genome of GAstV-JSXZ was 7299-nt-long. Interestingly, the phylogenetic analysis revealed that Chinese GAstV-1 has formed two distinct subgroups based on the ORF 2 genomes, designated GAstV-1 1a and GAstV-1 1b. The GAstV-JSXZ shared the highest identity with GAstV-1 1a strain FLX and TZ03 in nucleotides (ORF1a: 98.3\u201398.4%; ORF1b: 92.3\u201399.1%; ORF2: 95.8\u201398.8%) and amino acid sequences (ORF1a: 99.4\u201399.5%; ORF1b: 98.2\u201398.8%; ORF2: 97.0\u201399.4%). To evaluate the pathogenicity of GAstV-1, 1-day-old goslings were inoculated with the virus by oral and subcutaneous injection routes, respectively. The results revealed that the virus causes extensive pathological organ damage, especially in the kidney, liver, and thymus. Virus-specific genomic RNA could be detected in the cloacal swabs and tissues of infected goslings throughout the experiment. The viral copy numbers examined in the kidney and intestine were the highest, followed by the liver and spleen. These results are likely to provide a new understanding of the pathogenicity of GAstV-1 in geese.<\/jats:p>","DOI":"10.3390\/v16040541","type":"journal-article","created":{"date-parts":[[2024,3,31]],"date-time":"2024-03-31T13:44:52Z","timestamp":1711892692000},"page":"541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Isolation, Identification, and Pathogenicity of a Goose Astrovirus Genotype 1 Strain in Goslings in China"],"prefix":"10.3390","volume":"16","author":[{"given":"Feng","family":"Wei","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalin","family":"He","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingrong","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youxiang","family":"Diao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai\u2019an 271018, China"},{"name":"Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai\u2019an 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,30]]},"reference":[{"key":"ref_1","first-page":"1529","article-title":"Astrovirus Infections in Humans and Animals\u2014Molecular Biology, Genetic Diversity, and Interspecies Transmissions","volume":"1","author":"Burnham","year":"2011","journal-title":"Infect. 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