{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:22:35Z","timestamp":1781108555457,"version":"3.54.1"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-14-00245"],"award-info":[{"award-number":["21-14-00245"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE\/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to rhabdoviruses and iflaviruses, were detected in the IRE\/CTVM19 cell line, suggesting the presence of multiple persisting viruses. Subsequently, the full genome of an IRE\/CTVM19-associated rhabdovirus was recovered from a cell culture during the isolation of the Alongshan virus. In the current work, we used high-throughput sequencing to describe a virome of the IRE\/CTVM19 cell line. In addition to the previously detected IRE\/CTVM19-associated rhabdovirus, two rhabdoviruses were detected: Chimay rhabdovirus and Norway mononegavirus 1. In the follow-up experiments, we were able to detect both positive and negative RNA strands of the IRE\/CTVM19-associated rhabdovirus and Norway mononegavirus 1 in the IRE\/CTVM19 cells, suggesting their active replication in the cell line. Passaging attempts in cell lines of mammalian origin failed for all three discovered rhabdoviruses.<\/jats:p>","DOI":"10.3390\/v16040576","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T02:52:17Z","timestamp":1712631137000},"page":"576","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["High-Throughput Sequencing Reveals Three Rhabdoviruses Persisting in the IRE\/CTVM19 Cell Line"],"prefix":"10.3390","volume":"16","author":[{"given":"Alexander G.","family":"Litov","sequence":"first","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"},{"name":"Institute for Translational Medicine and Biotechnology, Sechenov University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1842-3899","authenticated-orcid":false,"given":"Alexey M.","family":"Shchetinin","sequence":"additional","affiliation":[{"name":"Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-7081","authenticated-orcid":false,"given":"Ivan S.","family":"Kholodilov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-0774","authenticated-orcid":false,"given":"Oxana A.","family":"Belova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1571-2313","authenticated-orcid":false,"given":"Magomed N.","family":"Gadzhikurbanov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Y.","family":"Ivannikova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-763X","authenticated-orcid":false,"given":"Anastasia A.","family":"Kovpak","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9397-3762","authenticated-orcid":false,"given":"Vladimir A.","family":"Gushchin","sequence":"additional","affiliation":[{"name":"Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina G.","family":"Karganova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"},{"name":"Institute for Translational Medicine and Biotechnology, Sechenov University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e12813","DOI":"10.1111\/pim.12813","article-title":"Update on the intricate tango between tick microbiomes and tick-borne pathogens","volume":"43","author":"Bonnet","year":"2020","journal-title":"Parasite Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101682","DOI":"10.1016\/j.ttbdis.2021.101682","article-title":"What do we still need to know about Ixodes ricinus?","volume":"12","author":"Gray","year":"2021","journal-title":"Ticks Tick. 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