{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:25:33Z","timestamp":1790717133478,"version":"4.1.0"},"reference-count":99,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>West Nile Virus (WNV) and Usutu Virus (USUV) are both neurotropic mosquito-borne viruses belonging to the Flaviviridae family. These closely related viruses mainly follow an enzootic cycle involving mosquitoes as vectors and birds as amplifying hosts, but humans and other mammals can also be infected through mosquito bites. WNV was first identified in Uganda in 1937 and has since spread globally, notably in Europe, causing periodic outbreaks associated with severe cases of neuroinvasive diseases such as meningitis and encephalitis. USUV was initially isolated in 1959 in Swaziland and has also spread to Europe, primarily affecting birds and having a limited impact on human health. There has been a recent expansion of these viruses\u2019 geographic range in Europe, facilitated by factors such as climate change, leading to increased human exposure. While sharing similar biological traits, ecology, and epidemiology, there are significant distinctions in their pathogenicity and their impact on both human and animal health. While WNV has been more extensively studied and is a significant public health concern in many regions, USUV has recently been gaining attention due to its emergence in Europe and the diversity of its circulating lineages. Understanding the pathophysiology, ecology, and transmission dynamics of these viruses is important to the implementation of effective surveillance and control measures. This perspective provides a brief overview of the current situation of these two viruses in Europe and outlines the significant challenges that need to be addressed in the coming years.<\/jats:p>","DOI":"10.3390\/v16040599","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T11:09:20Z","timestamp":1712920160000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":120,"title":["Circulation of West Nile Virus and Usutu Virus in Europe: Overview and Challenges"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3475-1369","authenticated-orcid":false,"given":"Yannick","family":"Simonin","sequence":"first","affiliation":[{"name":"Pathogenesis and Control of Chronic and Emerging Infections, University of Montpellier, INSERM, EFS, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/s41558-022-01426-1","article-title":"Over half of known human pathogenic diseases can be aggravated by climate change","volume":"12","author":"Mora","year":"2022","journal-title":"Nat. Clim. Chang."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jcv.2018.08.007","article-title":"Ongoing and emerging arbovirus threats in Europe","volume":"107","author":"Barzon","year":"2018","journal-title":"J. Clin. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-3527(03)59001-7","article-title":"Taxonomy of the virus family Flaviviridae","volume":"59","author":"Calisher","year":"2003","journal-title":"Adv. Virus Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1080\/00034983.1964.11686258","article-title":"The isolation of west nile virus from man and of usutu virus from the bird-biting mosquito mansonia aurites (theobald) in the entebbe area of uganda","volume":"58","author":"Williams","year":"1964","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"471","DOI":"10.4269\/ajtmh.1940.s1-20.471","article-title":"A neurotropic virus isolated from the blood of a native of Uganda","volume":"s1-20","author":"Smithburn","year":"1940","journal-title":"Am. Soc. Trop. Med. Hyg."},{"key":"ref_6","first-page":"1059","article-title":"Usutu (SAAr 1776); nouvel arbovirus du groupe B","volume":"3","author":"McIntosh","year":"1985","journal-title":"Int. Cat Arboviruses"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4869","DOI":"10.3390\/ijerph10104869","article-title":"European surveillance for West Nile virus in mosquito populations","volume":"10","author":"Engler","year":"2013","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e96","DOI":"10.1038\/emi.2017.82","article-title":"Vector competence of European mosquitoes for west Nile virus","volume":"6","author":"Vogels","year":"2017","journal-title":"Emerg. Microbes Infect."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.onehlt.2015.08.002","article-title":"Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe","volume":"1","author":"Fros","year":"2015","journal-title":"One Health"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Holicki, C.M., Ziegler, U., R\u0103ileanu, C., Kampen, H., Werner, D., Schulz, J., Silaghi, C., Groschup, M.H., and Vasi\u0107, A. (2020). West Nile Virus Lineage 2 Vector Competence of Indigenous Culex and Aedes Mosquitoes from Germany at Temperate Climate Conditions. Viruses, 12.","DOI":"10.3390\/v12050561"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1093\/jme\/tjaa262","article-title":"Higher West Nile Virus Infection in Aedes albopictus (Diptera: Culicidae) and Culex (Diptera: Culicidae) Mosquitoes From Lower Income Neighborhoods in Urban Baltimore, MD","volume":"58","author":"Rothman","year":"2021","journal-title":"J. Med. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Martinet, J.P., Bohers, C., Vazeille, M., Fert\u00e9, H., Mousson, L., Mathieu, B., Depaquit, J., and Failloux, A.B. (2023). Assessing vector competence of mosquitoes from northeastern France to West Nile virus and Usutu virus. PLoS Negl. Trop. Dis., 17.","DOI":"10.1101\/2023.02.07.527438"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13071-019-3316-z","article-title":"Targeted surveillance reveals native and invasive mosquito species infected with Usutu virus","volume":"12","author":"Camp","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"401","DOI":"10.3934\/mbe.2015009","article-title":"A mathematical model for the spread of west Nile virus in migratory and resident birds","volume":"13","author":"Bergsman","year":"2016","journal-title":"Math. Biosci. Eng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3201\/eid0903.020628","article-title":"Experimental infection of North American birds with the New York 1999 strain of West Nile virus","volume":"9","author":"Komar","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetmic.2013.03.005","article-title":"Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008\/2009","volume":"165","author":"Bakonyi","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.vetmic.2007.01.029","article-title":"Monitoring of Usutu virus activity and spread by using dead bird surveillance in Austria, 2003\u20132005","volume":"122","author":"Chvala","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1016\/S1286-4579(03)00204-1","article-title":"Usutu virus activity in Austria, 2001\u20132002","volume":"5","author":"Kolodziejek","year":"2003","journal-title":"Microbes Infect."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Becker, N., J\u00f6st, H., Ziegler, U., Eiden, M., H\u00f6per, D., Emmerich, P., Fichet-Calvet, E., Ehichioya, D.U., Czajka, C., and Gabriel, M. (2012). Epizootic emergence of Usutu virus in wild and captive birds in Germany. PLoS ONE, 7.","DOI":"10.1371\/annotation\/6841c4e1-58e6-4412-9b71-bd6bc8bbe549"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.jcpa.2004.03.004","article-title":"Pathology and viral distribution in fatal Usutu virus infections of birds from the 2001 and 2002 outbreaks in Austria","volume":"131","author":"Chvala","year":"2004","journal-title":"J. Comp. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.3201\/eid2212.161272","article-title":"Dual emergence of Usutu virus in common blackbirds, Eastern France, 2015","volume":"22","author":"Lecollinet","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"30391","DOI":"10.2807\/1560-7917.ES.2016.21.45.30391","article-title":"Widespread Usutu virus outbreak in birds in The Netherlands, 2016","volume":"21","author":"Rijks","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1089\/vbz.2011.0631","article-title":"Usutu virus in Africa","volume":"11","author":"Nikolay","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1093\/trstmh\/trv066","article-title":"A review of West Nile and Usutu virus co-circulation in Europe: How much do transmission cycles overlap?","volume":"109","author":"Nikolay","year":"2015","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Root, J.J., and Bosco-Lauth, A.M. (2019). West nile virus associations in wild mammals: An update. Viruses, 11.","DOI":"10.3390\/v11050459"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.vetmic.2015.02.005","article-title":"West Nile virus serosurveillance in pigs, wild boars, and roe deer in Serbia","volume":"176","author":"Saiz","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.3201\/eid2010.140909","article-title":"Usutu virus in bats, Germany, 2013","volume":"20","author":"Cadar","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Diagne, M.M., Ndione, M.H.D., Di Paola, N., Fall, G., Bedekelabou, A.P., Semb\u00e8ne, P.M., Faye, O., De Andrade Zanotto, P.M., and Sall, A.A. (2019). Usutu virus isolated from rodents in Senegal. Viruses, 11.","DOI":"10.3390\/v11020181"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ba\u017can\u00f3w, B., Jansen van Vuren, P., Szyma\u0144ski, P., Stygar, D., Fr\u0105cka, A., Twardo\u0144, J., Kozdrowski, R., and Paw\u0119ska, J. (2018). A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland. Viruses, 10.","DOI":"10.3390\/v10020087"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/tbed.12259","article-title":"First detection of co-circulation of West Nile and Usutu viruses in equids in the south-west of Tunisia","volume":"61","author":"Calistri","year":"2014","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1089\/vbz.2018.2337","article-title":"Serological Evidence of Mosquito-Borne Flaviviruses Circulation in Hunting Dogs in Campania Region, Italy","volume":"19","author":"Montagnaro","year":"2019","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1017\/S095026881600011X","article-title":"Seroprevalence of West Nile and Usutu viruses in military working horses and dogs, Morocco, 2012: Dog as an alternative WNV sentinel species?","volume":"144","author":"Durand","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zannoli, S., and Sambri, V. (2019). West nile virus and usutu virus co-circulation in europe: Epidemiology and implications. Microorganisms, 7.","DOI":"10.3390\/microorganisms7070184"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lustig, Y., Sofer, D., Bucris, E.D., and Mendelson, E. (2018). Surveillance and diagnosis of west nile virus in the face of flavivirus cross-reactivity. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02421"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.3201\/eid1108.050289a","article-title":"Epidemiology and transmission dynamics of West Nile virus disease","volume":"11","author":"Hayes","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1086\/518281","article-title":"The long-term outcomes of human West Nile virus infection","volume":"44","author":"Sejvar","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Pacenti, M., Sinigaglia, A., Franchin, E., Pagni, S., Lavezzo, E., Montarsi, F., Capelli, G., and Barzon, L. (2020). Human West Nile Virus Lineage 2 Infection: Epidemiological, Clinical, and Virological Findings. Viruses, 12.","DOI":"10.3390\/v12040458"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Sejvar, J.J. (2014). Clinical Manifestations and Outcomes of West Nile Virus Infection. Viruses, 6.","DOI":"10.3390\/v6020606"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e232","DOI":"10.1017\/S0950268819001213","article-title":"Usutu virus: A new threat?","volume":"147","author":"Beck","year":"2019","journal-title":"Epidemiol. Infect."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Cadar, D., and Simonin, Y. (2022). Human Usutu Virus Infections in Europe: A New Risk on Horizon?. Viruses, 15.","DOI":"10.3390\/v15010077"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0140-6736(98)03538-7","article-title":"West Nile encephalitis epidemic in southeastern Romania","volume":"352","author":"Tsai","year":"1998","journal-title":"Lancet"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3201\/eid0701.010118","article-title":"Outbreak of West Nile virus infection, Volgograd Region, Russia, 1999","volume":"7","author":"Platonov","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"692","DOI":"10.3201\/eid0704.017417","article-title":"West Nile outbreak in horses in southern France, 2000: The return after 35 years","volume":"7","author":"Murgue","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"European Centre for Disease Prevention and Control (ECDC) (2024, March 20). Surveillance, Prevention and Control of West Nile Virus and Usutu Virus Infections in the EU\/EEA. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/surveillance-prevention-and-control-west-nile-virus-and-usutu-virus-infections.","DOI":"10.2903\/sp.efsa.2023.EN-8242"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"652","DOI":"10.3201\/eid0807.020094","article-title":"Emergence of Usutu virus, an African mosquito-borne Flavivirus of the Japanese encephalitis virus group, central Europe","volume":"8","author":"Kolodziejek","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mBio.01938-15","article-title":"Reconstruction of the evolutionary history and dispersal of Usutu virus, a neglected emerging arbovirus in Europe and Africa","volume":"7","author":"Engel","year":"2016","journal-title":"MBio"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"274","DOI":"10.3201\/eid1902.121191","article-title":"Usutu virus, Italy, 1996","volume":"19","author":"Bakonyi","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2200068","DOI":"10.2807\/1560-7917.ES.2022.27.25.2200068","article-title":"One Health surveillance of West Nile and Usutu viruses: A repeated cross-sectional study exploring seroprevalence and endemicity in Southern France, 2016 to 2020","volume":"27","author":"Constant","year":"2022","journal-title":"Euro Surveill."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Veo, C., Della Ventura, C., Moreno, A., Rovida, F., Percivalle, E., Canziani, S., Torri, D., Calzolari, M., Baldanti, F., and Galli, M. (2019). Evolutionary Dynamics of the Lineage 2 West Nile Virus That Caused the Largest European Epidemic: Italy 2011\u20132018. Viruses, 11.","DOI":"10.3390\/v11090814"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Constant, O., Bollore, K., Cl\u00e9, M., Barthelemy, J., Foulongne, V., Chenet, B., Gomis, D., Virolle, L., Gutierrez, S., and Desmetz, C. (2020). Evidence of exposure to USUV and WNV in zoo animals in France. Pathogens, 9.","DOI":"10.3390\/pathogens9121005"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Beck, C., Goffart, I.L., Franke, F., Gonzalez, G., Dumarest, M., Lowenski, S., Blanchard, Y., Lucas, P., de Lamballerie, X., and Grard, G. (2020). Contrasted epidemiological patterns of west nile virus lineages 1 and 2 infections in France from 2015 to 2019. Pathogens, 9.","DOI":"10.3390\/pathogens9110908"},{"key":"ref_52","first-page":"2002010","article-title":"Unprecedented increase of West Nile virus neuroinvasive disease, Spain, summer 2020","volume":"26","author":"Moros","year":"2021","journal-title":"Eurosurveillance"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1900543","DOI":"10.2807\/1560-7917.ES.2020.25.32.1900543","article-title":"West Nile virus in humans, Greece, 2018: The largest seasonal number of cases, 9years after its emergence in the country","volume":"25","author":"Pervanidou","year":"2020","journal-title":"Eurosurveillance"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s41426-018-0021-5","article-title":"Integrated analysis of human-animal-vector surveillance: West Nile virus infections in Austria, 2015\u20132016","volume":"7","author":"Kolodziejek","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"106617","DOI":"10.1016\/j.ympev.2019.106617","article-title":"Evolutionary dynamics of lineage 2 West Nile virus in Europe, 2004\u20132018: Phylogeny, selection pressure and phylogeography","volume":"141","author":"Chaintoutis","year":"2019","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Srihi, H., Chatti, N., Ben Mhadheb, M., Gharbi, J., and Abid, N. (2021). Phylodynamic and phylogeographic analysis of the complete genome of the West Nile virus lineage 2 (WNV-2) in the Mediterranean basin. BMC Ecol. Evol., 21.","DOI":"10.1186\/s12862-021-01902-w"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"19762","DOI":"10.2807\/ese.16.02.19762-en","article-title":"Outbreak of West Nile virus infection in humans, Romania, July to October 2010","volume":"16","author":"Sirbu","year":"2011","journal-title":"Euro Surveill."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"618","DOI":"10.3201\/eid1204.051379","article-title":"Lineage 1 and 2 strains of encephalitic West Nile virus, Central Europe","volume":"12","author":"Bakonyi","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1111\/j.1939-1676.2011.0715.x","article-title":"Equine encephalomyelitis outbreak caused by a genetic lineage 2 west nile virus in Hungary","volume":"25","author":"Kutasi","year":"2011","journal-title":"J. Vet. Intern. Med."},{"key":"ref_60","first-page":"20613","article-title":"Outbreak of west Nile virus infection among humans in Serbia, August to October 2012","volume":"18","author":"Poluga","year":"2013","journal-title":"Eurosurveillance"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.vetmic.2012.02.018","article-title":"Evidence of West Nile virus lineage 2 circulation in Northern Italy","volume":"158","author":"Savini","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.3390\/v5112825","article-title":"Large human outbreak of West Nile virus infection in north-eastern Italy in 2012","volume":"5","author":"Barzon","year":"2013","journal-title":"Viruses"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1800545","DOI":"10.2807\/1560-7917.ES.2018.23.43.1800545","article-title":"Increase in human West Nile and Usutu virus infections, Austria, 2018","volume":"23","author":"Aberle","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/14787210.2020.1713751","article-title":"The knowns and unknowns of West Nile virus in Europe: What did we learn from the 2018 outbreak?","volume":"18","author":"Camp","year":"2020","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2200653","DOI":"10.2807\/1560-7917.ES.2022.27.36.2200653","article-title":"Rapid increase in neuroinvasive West Nile virus infections in humans, Italy, July 2022","volume":"27","author":"Riccardo","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_66","unstructured":"Aquitaine-Agence R\u00e9gionale de Sant\u00e9 Nouvelle (2024, March 20). Confirmation d\u2019une Infection Autochtone \u00e0 Virus Usutu (Secteurs des Landes et de Gironde). Available online: https:\/\/www.nouvelle-aquitaine.ars.sante.fr\/communique-de-presse-confirmation-dune-infection-autochtone-virus-usutu-secteurs-des-landes-et-de."},{"key":"ref_67","unstructured":"Gonzales, G., Bigeard, G., Mign\u00e9, C., Touzet, T., Fontaine, A., L\u2019Ambert, G., De Lamballerie, X., Zientera, S., Duvi-gnaud, A., and Malvy, D. (2024, March 20). Circulation Active du Virus West-Nile en Gironde, R\u00e9gion Nouvelle-Aquitaine. Available online: https:\/\/www.plateforme-esa.fr\/fr\/circulation-active-du-virus-west-nile-en-gironde-region-nouvelle-aquitaine-point-au-04-08-2023."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.3201\/eid2012.140921","article-title":"Putative new West Nile virus lineage in Uranotaenia unguiculata mosquitoes, Austria, 2013","volume":"20","author":"Pachler","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Habarugira, G., Suen, W.W., Hobson-Peters, J., Hall, R.A., and Bielefeldt-Ohmann, H. (2020). West nile virus: An update on pathobiology, epidemiology, diagnostics, control and \u201cOne health\u201d implications. Pathogens, 9.","DOI":"10.3390\/pathogens9070589"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"32","DOI":"10.14745\/ccdr.v45i01a04","article-title":"West Nile virus illness in Ontario, Canada: 2017","volume":"44","author":"Wijayasri","year":"2019","journal-title":"Can. Commun. Dis. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1684\/vir.2019.0787","article-title":"West Nile virus historical progression in Europe","volume":"23","author":"Martin","year":"2019","journal-title":"Virologie"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"30452","DOI":"10.2807\/1560-7917.ES.2017.22.4.30452","article-title":"Widespread activity of multiple lineages of Usutu virus, Western Europe, 2016","volume":"22","author":"Cadar","year":"2017","journal-title":"Eurosurveillance"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.meegid.2018.03.020","article-title":"Emergence of two Usutu virus lineages in Culex pipiens mosquitoes in the Camargue, France, 2015","volume":"61","author":"Eiden","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"875","DOI":"10.3201\/eid2405.171122","article-title":"Human Usutu virus infection with atypical neurologic presentation, Montpellier, France, 2016","volume":"24","author":"Simonin","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.vetmic.2016.06.007","article-title":"Evidence for an independent third Usutu virus introduction into Germany","volume":"192","author":"Ziegler","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Cl\u00e9, M., Barthelemy, J., Desmetz, C., Foulongne, V., Lapeyre, L., Bollor\u00e9, K., Tuaillon, E., Erkilic, N., Kalatzis, V., and Lecollinet, S. (2020). Study of Usutu virus neuropathogenicity in mice and human cellular models. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008223"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Cvjetkovi\u0107, I.H., Radovanov, J., Kova\u010devi\u0107, G., Turkulov, V., and Pati\u0107, A. (2023). Diagnostic value of urine qRT-PCR for the diagnosis of West Nile virus neuroinvasive disease. Diagn. Microbiol. Infect. Dis., 107.","DOI":"10.1016\/j.diagmicrobio.2023.115920"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Percivalle, E., Cassaniti, I., Sarasini, A., Rovida, F., Adzasehoun, K.M.G., Colombini, I., Isernia, P., Cuppari, I., and Baldanti, F. (2020). West nile or usutu virus? A three-year follow-up of humoral and cellular response in a group of asymptomatic blood donors. Viruses, 12.","DOI":"10.3390\/v12020157"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"17-00644","DOI":"10.2807\/1560-7917.ES.2017.22.41.17-00644","article-title":"Usutu virus infections among blood donors, Austria, july and august 2017\u2014Raising awareness for diagnostic challenges","volume":"22","author":"Bakonyi","year":"2017","journal-title":"Eurosurveillance"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12974-020-02060-4","article-title":"Differential neurovirulence of Usutu virus lineages in mice and neuronal cells","volume":"18","author":"Constant","year":"2021","journal-title":"J. Neuroinflammation"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Kuchinsky, S.C., Hawks, S.A., Mossel, E.C., Coutermarsh-Ott, S., and Duggal, N.K. (2020). Differential pathogenesis of Usutu virus isolates in mice. PLoS Negl. Trop. Dis., 14.","DOI":"10.1101\/2020.08.31.275149"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Gim\u00e9nez-Richarte, \u00c1., Ortiz de Salazar, M.I., Gim\u00e9nez-Richarte, M.P., Collado, M., Fern\u00e1ndez, P.L., Clavijo, C., Navarro, L., Arbona, C., Marco, P., and Ramos-Rincon, J.M. (2022). Transfusion-transmitted arboviruses: Update and systematic review. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010843"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"20758","DOI":"10.2807\/1560-7917.ES2014.19.13.20758","article-title":"West Nile virus outbreak in humans, Greece, 2012: Third consecutive year of local transmission","volume":"19","author":"Pervanidou","year":"2014","journal-title":"Euro Surveill."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.3201\/eid2506.181755","article-title":"West nile and usutu virus infections and challenges to blood safety in the European Union","volume":"25","author":"Gossner","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1093\/jme\/tjz125","article-title":"West Nile Virus: Veterinary Health and Vaccine Development","volume":"56","author":"Bowen","year":"2019","journal-title":"J. Med. Entomol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1111\/jvim.16581","article-title":"European College of Equine Internal Medicine consensus statement on equine flaviviridae infections in Europe","volume":"36","author":"Cavalleri","year":"2022","journal-title":"J. Vet. Intern. Med."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Saiz, J.C. (2020). Animal and Human Vaccines against West Nile Virus. Pathogens, 9.","DOI":"10.3390\/pathogens9121073"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1016\/j.vaccine.2016.03.015","article-title":"A recombinant DNA vaccine protects mice deficient in the alpha\/beta interferon receptor against lethal challenge with Usutu virus","volume":"34","author":"Sobrino","year":"2016","journal-title":"Vaccine"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Salgado, R., Hawks, S.A., Frere, F., V\u00e1zquez, A., Huang, C.Y.H., and Duggal, N.K. (2021). West Nile Virus Vaccination Protects against Usutu Virus Disease in Mice. Viruses, 13.","DOI":"10.3390\/v13122352"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Sun, H., Acharya, D., Paul, A.M., Lai, H., He, J., Bai, F., and Chen, Q. (2023). Antibody-Dependent Enhancement Activity of a Plant-Made Vaccine against West Nile Virus. Vaccines, 11.","DOI":"10.3390\/vaccines11020197"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Jurisic, L., Malatesta, D., Zaccaria, G., Di Teodoro, G., Bonfini, B., Valleriani, F., Teodori, L., Bencivenga, F., Leone, A., and Rip\u00e0, P. (2023). Immunization with Usutu virus and with a chimeric West Nile virus (WNV) harboring Usutu-E protein protects immunocompetent adult mice against lethal challenges with different WNV lineage 1 and 2 strains. Vet. Microbiol., 277.","DOI":"10.1016\/j.vetmic.2022.109636"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.virol.2015.03.020","article-title":"Limited susceptibility of mice to Usutu virus (USUV) infection and induction of flavivirus cross-protective immunity","volume":"482","author":"Petrovic","year":"2015","journal-title":"Virology"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Zaccaria, G., Malatesta, D., Jurisic, L., Marcacci, M., Di Teodoro, G., Conte, A., Teodori, L., Monaco, F., Marini, V., and Casaccia, C. (2021). The envelope protein of Usutu virus attenuates West Nile virus virulence in immunocompetent mice. Vet. Microbiol., 263.","DOI":"10.1016\/j.vetmic.2021.109262"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Merino-Ramos, T., Bl\u00e1zquez, A.-B., Escribano-Romero, E., Ca\u00f1 as-Arranz, R., Sobrino, F., Saiz, J.-C., Mart\u00edn-Acebes, M.A., Bl\u00e1zquez, A.-B., Escribano-Romero, E., and Ca\u00f1as-Arranz, R. (2014). Protection of a Single Dose West Nile Virus Recombinant Subviral Particle Vaccine against Lineage 1 or 2 Strains and Analysis of the Cross-Reactivity with Usutu Virus. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108056"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1900261","DOI":"10.2807\/1560-7917.ES.2019.24.21.1900261","article-title":"West Nile virus infection in individuals with pre-existing Usutu virus immunity, northern Italy, 2018","volume":"24","author":"Sinigaglia","year":"2019","journal-title":"Euro Surveill."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"437","DOI":"10.3389\/fvets.2019.00437","article-title":"Emerging Trends in the Epidemiology of West Nile and Usutu Virus Infections in Southern Europe","volume":"6","author":"Savic","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1111\/tbed.14050","article-title":"Co-infections: Simultaneous detections of West Nile virus and Usutu virus in birds from Germany","volume":"69","author":"Santos","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1080\/22221751.2020.1854623","article-title":"Competition between Usutu virus and West Nile virus during simultaneous and sequential infection of Culex pipiens mosquitoes","volume":"9","author":"Wang","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s12985-023-02206-9","article-title":"Cellular co-infections of West Nile virus and Usutu virus influence virus growth kinetics","volume":"20","author":"Reemtsma","year":"2023","journal-title":"Virol. J."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/4\/599\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:27:12Z","timestamp":1760106432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/4\/599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,12]]},"references-count":99,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["v16040599"],"URL":"https:\/\/doi.org\/10.3390\/v16040599","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,12]]}}}