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Orthobunyaviruses are widely distributed globally and pose threats to human and animal health. Ebinur Lake virus (EBIV) is a newly classified Orthobunyavirus detected in China, Russia, and Kenya. This study explored the antiviral effects of two broad-spectrum antiviral drugs, favipiravir and ribavirin, in a BALB\/c mouse model. Favipiravir significantly improved the clinical symptoms of infected mice, reduced viral titer and RNA copies in serum, and extended overall survival. The median survival times of mice in the vehicle- and favipiravir-treated groups were 5 and 7 days, respectively. Favipiravir significantly reduced virus titers 10- to 100-fold in sera at all three time points compared to vehicle-treated mice. And favipiravir treatment effectively reduced the virus copies by approximately 10-fold across the three time points, relative to vehicle-treated mice. The findings expand the antiviral spectrum of favipiravir for orthobunyaviruses in vivo.<\/jats:p>","DOI":"10.3390\/v16040631","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T12:15:32Z","timestamp":1713442532000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Favipiravir Treatment Prolongs Survival in a Lethal BALB\/c Mouse Model of Ebinur Lake Virus Infection"],"prefix":"10.3390","volume":"16","author":[{"given":"Jingke","family":"Geng","sequence":"first","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"},{"name":"University of Chinese Academy of Sciences, Beijing 101408, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanjie","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"},{"name":"University of Chinese Academy of Sciences, Beijing 101408, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cihan","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"},{"name":"University of Chinese Academy of Sciences, Beijing 101408, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doudou","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77551, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-9616","authenticated-orcid":false,"given":"Zhiming","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"},{"name":"University of Chinese Academy of Sciences, Beijing 101408, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-6040","authenticated-orcid":false,"given":"Han","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430200, China"},{"name":"University of Chinese Academy of Sciences, Beijing 101408, China"},{"name":"Hubei Jiangxia Laboratory, Wuhan 430207, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"001864","DOI":"10.1099\/jgv.0.001864","article-title":"Annual (2023) Taxonomic Update of RNA-Directed RNA Polymerase-Encoding Negative-Sense RNA Viruses (Realm Riboviria: Kingdom Orthornavirae: Phylum Negarnaviricota)","volume":"104","author":"Kuhn","year":"2023","journal-title":"J. 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