{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:01:10Z","timestamp":1760144470947,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"QIAGEN Manchester Ltd., Manchester, UK"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>DNA assays for viral load (VL) monitoring are key tools in the management of immunocompromised patients with cytomegalovirus (CMV) or Epstein\u2013Barr virus (EBV) infection. In this study, the analytical and clinical performances of the NeuMoDx\u2122 CMV and EBV Quant Assays were compared with artus CMV and EBV QS-RGQ Kits in a primary hospital testing laboratory. Patient plasma samples previously tested using artus kits were randomly selected for testing by NeuMoDx assays. The NeuMoDx CMV Quant Assay and artus CMV QS-RGQ Kit limits of detection (LoDs) are 20.0 IU\/mL and 69.7 IU\/mL, respectively; 33\/75 (44.0%) samples had CMV DNA levels above the LoD of both assays. The Pearson correlation coefficient was 0.9503; 20 samples (60.6%) had lower NeuMoDx CMV quantification values versus the artus kit. The LoD of the NeuMoDx EBV Quant Assay and artus EBV QS-RGQ Kit are 200 IU\/mL and 22.29 IU\/mL, respectively; 16\/75 (21.3%) samples had EBV DNA levels above the LoD of both assays. The Pearson correlation coefficient was 0.8990. EBV quantification values with the NeuMoDx assay were higher versus the artus kit in 15 samples (93.8%). In conclusion, NeuMoDx CMV and EBV Quant Assays are sensitive and accurate tools for CMV and EBV DNA VL quantification.<\/jats:p>","DOI":"10.3390\/v16050671","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T09:26:02Z","timestamp":1714123562000},"page":"671","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Analytical and Clinical Performance of the NeuMoDx\u2122 Platform for Cytomegalovirus and Epstein\u2013Barr Virus Viral Load Testing"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2452-105X","authenticated-orcid":false,"given":"Lindsay","family":"Coupland","sequence":"first","affiliation":[{"name":"Microbiology Department, Eastern Pathology Alliance, Norfolk and Norwich University Hospital NHS Foundation Trust, NRP Innovation Centre, Norwich Research Park, Colney, Norwich NR4 7GJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katy","family":"Woodward","sequence":"additional","affiliation":[{"name":"Microbiology Department, Eastern Pathology Alliance, Norfolk and Norwich University Hospital NHS Foundation Trust, NRP Innovation Centre, Norwich Research Park, Colney, Norwich NR4 7GJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0633-8611","authenticated-orcid":false,"given":"Samir","family":"Dervisevic","sequence":"additional","affiliation":[{"name":"Microbiology Department, Eastern Pathology Alliance, Norfolk and Norwich University Hospital NHS Foundation Trust, NRP Innovation Centre, Norwich Research Park, Colney, Norwich NR4 7GJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5398-2744","authenticated-orcid":false,"given":"Rachel","family":"Hale","sequence":"additional","affiliation":[{"name":"Microbiology Department, Eastern Pathology Alliance, Norfolk and Norwich University Hospital NHS Foundation Trust, NRP Innovation Centre, Norwich Research Park, Colney, Norwich NR4 7GJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Brolly","sequence":"additional","affiliation":[{"name":"Microbiology Department, Eastern Pathology Alliance, Norfolk and Norwich University Hospital NHS Foundation Trust, NRP Innovation Centre, Norwich Research Park, Colney, Norwich NR4 7GJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1056\/NEJM200008173430707","article-title":"Epstein-Barr virus infection","volume":"343","author":"Cohen","year":"2000","journal-title":"N. 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