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drugs\u2019","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"National Natural Science Foundation of China (NSFC) \u2018Genetic modification of herpesvirus as a delivery system for high expression of small molecule drugs\u2019","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["NJYT22043"],"award-info":[{"award-number":["NJYT22043"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["BR220113"],"award-info":[{"award-number":["BR220113"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["32260894"],"award-info":[{"award-number":["32260894"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Inner Mongolia Natural Science Foundation","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["NJYT22043"],"award-info":[{"award-number":["NJYT22043"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["BR220113"],"award-info":[{"award-number":["BR220113"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["32260894"],"award-info":[{"award-number":["32260894"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that causes severe abortions in sows and high piglet mortality, resulting in huge economic losses to the pig industry worldwide. The emerging and novel PRRSV isolates are clinically and biologically important, as there are likely recombination and pathogenic differences among PRRSV genomes. Furthermore, the NADC34-like strain has become a major epidemic strain in some parts of China, but the characterization and pathogenicity of the latest strain in Inner Mongolia have not been reported in detail. In this study, an NADC34-like strain (CHNMGKL1-2304) from Tongliao City, Inner Mongolia was successfully isolated and characterized, and confirmed the pathogenicity in pigs. The phylogenetic tree showed that this strain belonged to sublineage 1.5 and had high homology with the strain JS2021NADC34. There is no recombination between CHNMGKL1-2304 and any other domestic strains. Animal experiments show that the CHNMGKL1-2304 strain is moderately virulent to piglets, which show persistent fever, weight loss and high morbidity but no mortality. The presence of PRRSV nucleic acids was detected in both blood, tissues, nasal and fecal swabs. In addition, obvious pathological changes and positive signals were observed in lung, lymph node, liver and spleen tissues when subjected to hematoxylin\u2013eosin (HE) staining and immunohistochemistry (IHC). This report can provide a basis for epidemiological investigations and subsequent studies of PRRSV.<\/jats:p>","DOI":"10.3390\/v16050683","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T09:26:02Z","timestamp":1714123562000},"page":"683","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evolution Characterization and Pathogenicity of an NADC34-like PRRSV Isolated from Inner Mongolia, China"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6654-9923","authenticated-orcid":false,"given":"Hong-Zhe","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical Experiment Center, Inner Mongolia Medical University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Meng","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Long","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Wen","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zou","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Ye","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Qi","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Dong","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, 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