{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:15:58Z","timestamp":1785816958239,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["NYCV478910"],"award-info":[{"award-number":["NYCV478910"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["NPB#22-081"],"award-info":[{"award-number":["NPB#22-081"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008370","name":"National Pork Board","doi-asserted-by":"publisher","award":["NYCV478910"],"award-info":[{"award-number":["NYCV478910"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008370","name":"National Pork Board","doi-asserted-by":"publisher","award":["NPB#22-081"],"award-info":[{"award-number":["NPB#22-081"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cornell Atkinson Center for Sustainability\u2014Graduate Research Grant in Food Security","award":["NYCV478910"],"award-info":[{"award-number":["NYCV478910"]}]},{"name":"Cornell Atkinson Center for Sustainability\u2014Graduate Research Grant in Food Security","award":["NPB#22-081"],"award-info":[{"award-number":["NPB#22-081"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>African Swine Fever Virus (ASFV) is a large dsDNA virus that encodes at least 150 proteins. The complexity of ASFV and lack of knowledge of effector immune functions and protective antigens have hindered the development of safe and effective ASF vaccines. In this study, we constructed four Orf virus recombinant vectors expressing individual ASFV genes B602L, -CP204L, E184L, and -I73R (ORFV\u0394121-ASFV-B602L, -CP204L, -E184L, and -I73R). All recombinant viruses expressed the heterologous ASFV proteins in vitro. We then evaluated the immunogenicity of the recombinants by immunizing four-week-old piglets. In two independent animal studies, we observed high antibody titers against ASFV p30, encoded by CP204L gene. Using Pepscan ELISA, we identified a linear B-cell epitope of 12 amino acids in length (Peptide 15) located in an exposed loop region of p30 as an immunodominant ASFV epitope. Additionally, antibodies elicited against ASFV p30 presented antibody-dependent cellular cytotoxicity (ADCC) activity. These results underscore the role of p30 on antibody responses elicited against ASFV and highlight an important functional epitope that contributes to p30-specific antibody responses.<\/jats:p>","DOI":"10.3390\/v16050758","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T10:26:36Z","timestamp":1715682396000},"page":"758","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of an Immunodominant B-Cell Epitope in African Swine Fever Virus p30 Protein and Evidence of p30 Antibody-Mediated Antibody Dependent Cellular Cytotoxicity"],"prefix":"10.3390","volume":"16","author":[{"given":"Jessica C. G.","family":"Noll","sequence":"first","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruchi","family":"Rani","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4565-0125","authenticated-orcid":false,"given":"Salman L.","family":"Butt","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7126-6410","authenticated-orcid":false,"given":"Maureen Hoch Vieira","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Mansano","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8290-5756","authenticated-orcid":false,"given":"Mathias","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo C.","family":"Caserta","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Covaleda","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3237-8940","authenticated-orcid":false,"given":"Diego G.","family":"Diel","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.chom.2019.11.004","article-title":"Cryo-EM Structure of the African Swine Fever Virus","volume":"26","author":"Liu","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1074\/jbc.AC119.011196","article-title":"The Cryo-EM Structure of African Swine Fever Virus Unravels a Unique Architecture Comprising Two Icosahedral Protein Capsids and Two Lipoprotein Membranes","volume":"295","author":"Charro","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.virusres.2012.10.026","article-title":"Pathogenesis of African Swine Fever in Domestic Pigs and European Wild Boar","volume":"173","author":"Blome","year":"2013","journal-title":"Virus Res."},{"key":"ref_4","unstructured":"King, A.M.Q., Adams, M.J., Carstens, E.B., and Lefkowitz, E.J. (2012). Virus Taxonomy, Elsevier."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0368-1742(21)80031-4","article-title":"On A Form of Swine Fever Occurring in British East Africa (Kenya Colony)","volume":"34","year":"1921","journal-title":"J. Comp. Pathol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Avil\u00e9s, M. (2023). African Swine Fever: Epidemiology, the Design of New Diagnostic Methods, and Vaccine Development. Pathogens, 12.","DOI":"10.3390\/pathogens12081042"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1038\/s43016-021-00362-1","article-title":"African Swine Fever Outbreaks in China Led to Gross Domestic Product and Economic Losses","volume":"2","author":"You","year":"2021","journal-title":"Nat. Food"},{"key":"ref_8","unstructured":"(2024, January 04). FAO African Swine Fever (ASF) Situation Update in Asia & Pacific. Available online: https:\/\/www.fao.org\/animal-health\/situation-updates\/asf-in-asia-pacific."},{"key":"ref_9","unstructured":"(2024, January 04). USDA USDA Statement on Confirmation of African Swine Fever in Haiti, Available online: https:\/\/www.aphis.usda.gov\/aphis\/newsroom\/stakeholder-info\/sa_by_date\/sa-2021\/sa-09\/asf-haiti."},{"key":"ref_10","unstructured":"(2024, January 04). USDA USDA Statement on Confirmation of African Swine Fever in the Dominican Republic, Available online: https:\/\/content.govdelivery.com\/accounts\/USDAAPHIS\/bulletins\/2ea72f5?reqfrom=share\/."},{"key":"ref_11","unstructured":"(2024, January 04). USDA Sweden: First Case of African Swine Fever Found in Wild Boars in Sweden, Available online: https:\/\/fas.usda.gov\/data\/sweden-first-case-african-swine-fever-found-wild-boars-sweden."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rock, D.L. (2021). Thoughts on African Swine Fever Vaccines. Viruses, 13.","DOI":"10.3390\/v13050943"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.1016\/j.vaccine.2011.04.052","article-title":"Protection of European Domestic Pigs from Virulent African Isolates of African Swine Fever Virus by Experimental Immunisation","volume":"29","author":"King","year":"2011","journal-title":"Vaccine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23476","DOI":"10.1038\/s41598-021-02949-x","article-title":"Optimization in the Expression of ASFV Proteins for the Development of Subunit Vaccines Using Poxviruses as Delivery Vectors","volume":"11","author":"Gladue","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_15","unstructured":"(2024, January 04). Reuters Vietnam Suspends African Swine Fever Vaccine after Pig Deaths. Available online: https:\/\/www.reuters.com\/world\/asia-pacific\/vietnam-suspends-african-swine-fever-vaccine-after-pig-deaths-2022-08-24\/."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1099\/0022-1317-82-3-513","article-title":"The Non-Haemadsorbing African Swine Fever Virus Isolate ASFV\/NH\/P68 Provides a Model for Defining the Protective Anti-Virus Immune Response","volume":"82","author":"Cartaxeiro","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"367","DOI":"10.3181\/00379727-134-34794","article-title":"Hypergammaglobulinemia in Swine Infected with African Swine Fever Virus","volume":"134","author":"Pan","year":"1970","journal-title":"Exp. Biol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1099\/0022-1317-80-6-1453","article-title":"African Swine Fever Virus: A B Cell-Mitogenic Virus in Vivo and in Vitro","volume":"80","author":"Takamatsu","year":"1999","journal-title":"J. Gen. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gaudreault, N.N., and Richt, J.A. (2019). Subunit Vaccine Approaches for African Swine Fever Virus. Vaccines, 7.","DOI":"10.3390\/vaccines7020056"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12260","DOI":"10.1128\/JVI.01323-06","article-title":"The African Swine Fever Virus Nonstructural Protein PB602L Is Required for Formation of the Icosahedral Capsid of the Virus Particle","volume":"80","author":"Epifano","year":"2006","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7221","DOI":"10.1128\/JVI.75.16.7221-7229.2001","article-title":"A Virally Encoded Chaperone Specialized for Folding of the Major Capsid Protein of African Swine Fever Virus","volume":"75","author":"Cobbold","year":"2001","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1007\/s00705-008-0246-z","article-title":"Expression, Cellular Localization and Antibody Responses of the African Swine Fever Virus Genes B602L and K205R","volume":"153","author":"Reis","year":"2008","journal-title":"Arch. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/JVI.01293-18","article-title":"A Proteomic Atlas of the African Swine Fever Virus Particle","volume":"92","author":"Alejo","year":"2018","journal-title":"J. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e0141921","DOI":"10.1128\/jvi.00545-22","article-title":"Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge","volume":"96","author":"Vuono","year":"2022","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1099\/0022-1317-83-6-1331","article-title":"Identification of the Principal Serological Immunodeterminants of African Swine Fever Virus by Screening a Virus CDNA Library with Antibody","volume":"83","author":"Kollnberger","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.3389\/fimmu.2019.01318","article-title":"Identification and Immunogenicity of African Swine Fever Virus Antigens","volume":"10","author":"Netherton","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"E1923","DOI":"10.1111\/tbed.14527","article-title":"Structural Insight into African Swine Fever Virus I73R Protein Reveals It as a Z-DNA Binding Protein","volume":"69","author":"Sun","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/JVI.00119-20","article-title":"The African Swine Fever Virus Transcriptome","volume":"94","author":"Cackett","year":"2020","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e2210808120","DOI":"10.1073\/pnas.2210808120","article-title":"African Swine Fever Virus I73R Is a Critical Virulence-Related Gene: A Potential Target for Attenuation","volume":"120","author":"Liu","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0042-6822(92)90718-5","article-title":"Characterization of P30, a Highly Antigenic Membrane and Secreted Protein of African Swine Fever Virus","volume":"189","author":"Afonso","year":"1992","journal-title":"Virology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/jvi.67.5.2475-2485.1993","article-title":"Sequence and Characterization of the Major Early Phosphoprotein P32 of African Swine Fever Virus","volume":"67","author":"Prados","year":"1993","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, M., Song, J., Sun, J., Du, Y., Qin, X., Xia, L., Wu, Y., and Zhang, G. (2022). Development of an Effective Double Antigen Sandwich ELISA Based on P30 Protein to Detect Antibodies against African Swine Fever Virus. Viruses, 14.","DOI":"10.3390\/v14102170"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/JVI.78.1.168-177.2004","article-title":"Genomes of the Parapoxviruses Orf Virus and Bovine Papular Stomatitis Virus","volume":"78","author":"Delhon","year":"2004","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jviromet.2004.08.009","article-title":"A Natural Vaccinia Virus Promoter with Exceptional Capacity to Direct Protein Synthesis","volume":"122","author":"Liu","year":"2004","journal-title":"J. Virol. Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1128\/JVI.02236-10","article-title":"Orf Virus ORFV121 Encodes a Novel Inhibitor of NF-\u039aB That Contributes to Virus Virulence","volume":"85","author":"Diel","year":"2011","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/0022-2836(81)90375-2","article-title":"Bacteriophage P1 Site-Specific Recombination","volume":"150","author":"Sternberg","year":"1981","journal-title":"J. Mol. Biol."},{"key":"ref_37","unstructured":"Hierholzer, J.C., and Killington, R.A. (1996). Virology Methods Manual, Elsevier."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s11262-021-01879-0","article-title":"A Systematic Review of Genotypes and Serogroups of African Swine Fever Virus","volume":"58","author":"Qu","year":"2022","journal-title":"Virus Genes."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1038\/s41596-022-00728-0","article-title":"I-TASSER-MTD: A Deep-Learning-Based Platform for Multi-Domain Protein Structure and Function Prediction","volume":"17","author":"Zhou","year":"2022","journal-title":"Nat. Protoc."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: Homology Modelling of Protein Structures and Complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1002\/prot.21688","article-title":"Exploring the Extremes of Sequence\/Structure Space with Ensemble Fold Recognition in the Program Phyre","volume":"70","author":"Herbert","year":"2008","journal-title":"Proteins"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/jcc.20291","article-title":"GROMACS: Fast, Flexible, and Free","volume":"26","author":"Lindahl","year":"2005","journal-title":"J. Comput. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"7182","DOI":"10.1063\/1.328693","article-title":"Polymorphic Transitions in Single Crystals: A New Molecular Dynamics Method","volume":"52","author":"Parrinello","year":"1981","journal-title":"J. Appl. Phys."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle Mesh Ewald: An N \u22c5log(N) Method for Ewald Sums in Large Systems","volume":"98","author":"Darden","year":"1993","journal-title":"J Chem Phys"},{"key":"ref_47","unstructured":"DeLano, W.L. (2024, March 03). The PyMOL Molecular Graphics System. Available online: http:\/\/www.Pymol.Org\/."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.celrep.2016.04.044","article-title":"TET2 Regulates Mast Cell Differentiation and Proliferation through Catalytic and Non-Catalytic Activities","volume":"15","author":"Montagner","year":"2016","journal-title":"Cell Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1099\/vir.0.070508-0","article-title":"Related Strains of African Swine Fever Virus with Different Virulence: Genome Comparison and Analysis","volume":"96","author":"Portugal","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Joshi, L.R., Knudsen, D., Pi\u00f1eyro, P., Dhakal, S., Renukaradhya, G.J., and Diel, D.G. (2021). Protective Efficacy of an Orf Virus-Vector Encoding the Hemagglutinin and the Nucleoprotein of Influenza A Virus in Swine. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.747574"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.virol.2017.08.027","article-title":"Immunogenicity of ORFV-Based Vectors Expressing the Rabies Virus Glycoprotein in Livestock Species","volume":"511","author":"Martins","year":"2017","journal-title":"Virology"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"do Nascimento, G.M., Bugybayeva, D., Patil, V., Schrock, J., Yadagiri, G., Renukaradhya, G.J., and Diel, D.G. (2023). An Orf-Virus (ORFV)-Based Vector Expressing a Consensus H1 Hemagglutinin Provides Protection against Diverse Swine Influenza Viruses. Viruses, 15.","DOI":"10.3390\/v15040994"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1099\/jgv.0.000586","article-title":"Immunogenicity of a Recombinant Parapoxvirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus","volume":"97","author":"Hain","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1007\/s00705-018-3855-1","article-title":"Passive Immunity to Porcine Epidemic Diarrhea Virus Following Immunization of Pregnant Gilts with a Recombinant Orf Virus Vector Expressing the Spike Protein","volume":"163","author":"Joshi","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"do Nascimento, G.M., de Oliveira, P.S.B., Butt, S.L., and Diel, D.G. (2024). Immunogenicity of Chimeric Hemagglutinins Delivered by an Orf-Virusvector Platform against Swine Influenza Virus in Pigs. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1322879"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1006\/viro.1994.1040","article-title":"Passively Transferred African Swine Fever Virus Antibodies Protect Swine against Lethal Infection","volume":"198","author":"Onisk","year":"1994","journal-title":"Virology"},{"key":"ref_57","first-page":"1361","article-title":"African Swine Fever Convalescent Sows: Subsequent Pregnancy and the Effect of Colostral Antibody on Challenge Inoculation of Their Pigs","volume":"45","author":"Schlafer","year":"1984","journal-title":"Am. J. Vet. Res."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Goatley, L.C., Nash, R.H., Andrews, C., Hargreaves, Z., Tng, P., Reis, A.L., Graham, S.P., and Netherton, C.L. (2022). Cellular and Humoral Immune Responses after Immunisation with Low Virulent African Swine Fever Virus in the Large White Inbred Babraham Line and Outbred Domestic Pigs. Viruses, 14.","DOI":"10.3390\/v14071487"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Silva, E.B., Krug, P.W., Ramirez-Medina, E., Valladares, A., Rai, A., Espinoza, N., Gladue, D.P., and Borca, M.V. (2022). The Presence of Virus Neutralizing Antibodies Is Highly Associated with Protection against Virulent Challenge in Domestic Pigs Immunized with ASFV Live Attenuated Vaccine Candidates. Pathogens, 11.","DOI":"10.3390\/pathogens11111311"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1007\/s007050170056","article-title":"Antigenic and Immunogenic Properties of a Chimera of Two Immunodominant African Swine Fever Virus Proteins","volume":"146","author":"Barderas","year":"2001","journal-title":"Arch. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1006\/viro.1998.9068","article-title":"The African Swine Fever Virus Proteins P54 and P30 Are Involved in Two Distinct Steps of Virus Attachment and Both Contribute to the Antibody-Mediated Protective Immune Response","volume":"243","author":"Oviedo","year":"1998","journal-title":"Virology"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.virol.2003.11.011","article-title":"Neutralizing Antibodies to African Swine Fever Virus Proteins P30, P54, and P72 Are Not Sufficient for Antibody-Mediated Protection","volume":"319","author":"Neilan","year":"2004","journal-title":"Virology"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0034-5288(18)32207-0","article-title":"Effector Mechanisms in the Pig. Antibody-Dependent Cellular Cytolysis of African Swine Fever Virus Infected Cells","volume":"35","author":"Norley","year":"1983","journal-title":"Res. Vet. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0378-1135(83)90063-9","article-title":"Interactions of Porcine Alveolar Macrophages and Bone Marrow Cells with African Swine Fever Virus and Virus-Infected Cells","volume":"8","author":"Forman","year":"1983","journal-title":"Vet. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0165-2427(85)90071-6","article-title":"The Role of Antibody in Protection against African Swine Fever Virus","volume":"9","author":"Wardley","year":"1985","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s00705-018-4071-8","article-title":"Evaluation of an African Swine Fever (ASF) Vaccine Strategy Incorporating Priming with an Alphavirus-Expressed Antigen Followed by Boosting with Attenuated ASF Virus","volume":"164","author":"Murgia","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"197632","DOI":"10.1016\/j.virusres.2019.05.010","article-title":"Development and Characterization of Monoclonal Antibodies against P30 Protein of African Swine Fever Virus","volume":"269","author":"Petrovan","year":"2019","journal-title":"Virus Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"199328","DOI":"10.1016\/j.virusres.2024.199328","article-title":"Identification of a Novel Linear B-Cell Epitope on the P30 Protein of African Swine Fever Virus Using Monoclonal Antibodies","volume":"341","author":"Tian","year":"2024","journal-title":"Virus Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1080\/22221751.2021.1999779","article-title":"Genotype I African Swine Fever Viruses Emerged in Domestic Pigs in China and Caused Chronic Infection","volume":"10","author":"Sun","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Fiori, M.S., Sanna, D., Scarpa, F., Floris, M., Di Nardo, A., Ferretti, L., Loi, F., Cappai, S., Sechi, A.M., and Angioi, P.P. (2021). A Deeper Insight into Evolutionary Patterns and Phylogenetic History of ASFV Epidemics in Sardinia (Italy) through Extensive Genomic Sequencing. 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