{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:09:13Z","timestamp":1760144953209,"version":"build-2065373602"},"reference-count":12,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Clinical Research Program for Child Health and Development of the Japan Agency for Medical Research and Development (AMED)","award":["24gn0110061h0003"],"award-info":[{"award-number":["24gn0110061h0003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Congenital cytomegalovirus (cCMV) infection poses significant risks to fetal development, particularly affecting the nervous system. This study reports a fetal autopsy case, examining cCMV infection and focusing on CMV DNA measurements in various fetal organs before formalin fixation, a novel approach for comprehensive CMV DNA evaluations in fetal organs affected by cCMV. A 20-week-old male fetus was diagnosed with cCMV following the detection of CMV DNA in ascites obtained via abdominocentesis in utero. After the termination of pregnancy, multiple organs of the fetus, including the cerebrum, thyroid gland, heart, lungs, liver, spleen, kidneys, and adrenal glands, were extracted and examined for CMV DNA loads using a real-time polymerase chain reaction. Histopathological examination involved hematoxylin\u2013eosin and CMV-specific immunostaining. A correlation was found between CMV DNA loads and pathology, with higher CMV-infected cell numbers observed in organs positively identified with both staining methods, exhibiting CMV DNA levels of \u22651.0 \u00d7 104 copies\/mL, compared to those detected solely by CMV-specific immunostaining, where CMV DNA levels ranged from 1.0 \u00d7 103 to 1.0 \u00d7 104 copies\/mL. These results highlight a quantifiable relationship between the organ infection extent and CMV DNA concentration, providing insights into cCMV pathogenesis and potentially informing future diagnostic and therapeutic strategies for cCMV infection.<\/jats:p>","DOI":"10.3390\/v16060891","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T12:19:46Z","timestamp":1717417186000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytomegalovirus DNA Loads in Organs of Congenitally Infected Fetus"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-1808","authenticated-orcid":false,"given":"Kuniaki","family":"Toriyabe","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asa","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"},{"name":"Department of Obstetrics and Gynecology, National Hospital Organization Mie Chuo Medical Center, Tsu 514-1101, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Ikejiri","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Mie University Hospital, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9237-029X","authenticated-orcid":false,"given":"Ryotaro","family":"Hashizume","sequence":"additional","affiliation":[{"name":"Department of Pathology and Matrix Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"},{"name":"Department of Genomic Medicine, Mie University Hospital, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Mie University Hospital, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emi","family":"Teramoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Mie University Hospital, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5994-7416","authenticated-orcid":false,"given":"Hiroki","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9773-2520","authenticated-orcid":false,"given":"Eiji","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoaki","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kitamura, A., Toriyabe, K., Hagimoto-Akasaka, M., Hamasaki-Shimada, K., Ikejiri, M., Minematsu, T., Suga, S., Kondo, E., Kihira, M., and Morikawa, F. (2023). Revision of Cytomegalovirus Immunoglobulin M Antibody Titer Cutoff in a Maternal Antibody Screening Program in Japan: A Cohort Comparison Involving a Total of 32,000 Pregnant Women. Viruses, 15.","DOI":"10.3390\/v15040962"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e000118","DOI":"10.1136\/bmjopen-2011-000118","article-title":"Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: Feasibility and outcomes from a multicentre study","volume":"1","author":"Koyano","year":"2011","journal-title":"BMJ Open"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.ajog.2020.02.018","article-title":"Cytomegalovirus Infection during Pregnancy: State of the Science","volume":"223","author":"Foulon","year":"2020","journal-title":"Am. J. Obstet. 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Sci., 25.","DOI":"10.3390\/ijms25052636"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/6\/891\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:51:42Z","timestamp":1760107902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/6\/891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":12,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["v16060891"],"URL":"https:\/\/doi.org\/10.3390\/v16060891","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2024,5,31]]}}}