{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:34:58Z","timestamp":1769643298015,"version":"3.49.0"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RSF-DST","award":["22-44-02007"],"award-info":[{"award-number":["22-44-02007"]}]},{"name":"RSF-DST","award":["DST\/INT\/RUS\/RSF\/P-51\/2021"],"award-info":[{"award-number":["DST\/INT\/RUS\/RSF\/P-51\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Puumala orthohantavirus (PUUV) is an emerging zoonotic virus endemic to Europe and Russia that causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS). There are limited options for treatment and diagnosis of orthohantavirus infection, making the search for potential immunogenic candidates crucial. In the present work, various bioinformatics tools were employed to design conserved immunogenic peptides containing multiple epitopes of PUUV nucleocapsid protein. Eleven conserved peptides (90% conservancy) of the PUUV nucleocapsid protein were identified. Three conserved peptides containing multiple T and B cell epitopes were selected using a consensus epitope prediction algorithm. Molecular docking using the HPEP dock server demonstrated strong binding interactions between the epitopes and HLA molecules (ten alleles for each class I and II HLA). Moreover, an analysis of population coverage using the IEDB database revealed that the identified peptides have over 90% average population coverage across six continents. Molecular docking and simulation analysis reveal a stable interaction with peptide constructs of chosen immunogenic peptides and Toll-like receptor-4. These computational analyses demonstrate selected peptides\u2019 immunogenic potential, which needs to be validated in different experimental systems.<\/jats:p>","DOI":"10.3390\/v16071030","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T09:29:33Z","timestamp":1719394173000},"page":"1030","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus"],"prefix":"10.3390","volume":"16","author":[{"given":"Ayushi","family":"Sehgal","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3333-3471","authenticated-orcid":false,"given":"Diksha","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neha","family":"Kaushal","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogita","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Martynova","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4328-8190","authenticated-orcid":false,"given":"Emmanuel","family":"Kabwe","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Chandy","sequence":"additional","affiliation":[{"name":"Childs Trust Medical Research Foundation (CTMRF) Kanchi, Chennai 600034, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9427-5739","authenticated-orcid":false,"given":"Albert","family":"Rizvanov","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Khaiboullina","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6581-9346","authenticated-orcid":false,"given":"Manoj","family":"Baranwal","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Davidyuk, Y.N., Kabwe, E., Shakirova, V.G., Martynova, E.V., Ismagilova, R.K., Khaertynova, I.M., Khaiboullina, S.F., Rizvanov, A.A., and Morzunov, S.P. 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