{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:09:46Z","timestamp":1773706186679,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"a scientific and technological exchange agreement between Vale S.A\u2014Vale, Salobo metais S.A\u2014Salobo, and the Evandro Chagas Institute\u2014IEC","doi-asserted-by":"publisher","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"a scientific and technological exchange agreement between Vale S.A\u2014Vale, Salobo metais S.A\u2014Salobo, and the Evandro Chagas Institute\u2014IEC","doi-asserted-by":"publisher","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"INCT em viroses emergentes e reemergentes (INCT-VER) of CNPq","doi-asserted-by":"publisher","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"INCT em viroses emergentes e reemergentes (INCT-VER) of CNPq","doi-asserted-by":"publisher","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In this study, we provide a genomic description of the first isolation of the Umattila virus (UMAV) in Brazil. The virus was obtained from the blood of a bird (Turdus fumigatus) and isolated in a C6\/36 cell culture. The viral genome contains ten segments, and its organization is characteristic of viruses of the genus Orbivirus (family Sedoreoviridae). The coding region of each segment was sequenced, demonstrating the nucleotide identity with UMAV. The phylogenetic inference results were in line with these findings and demonstrated the formation of two distinct monophyletic clades containing strains isolated around the world, where our isolate, belonging to the same clade as the prototype strain, was allocated to a different subclade, highlighting the genetic divergence between them. This work reports the first isolation of UMAV in Brazil, and due to the scarcity of information on this viral agent in the scientific literature, it is essential to carry out further studies to better understand its epidemiology, dispersion, and, in particular, its interactions with vertebrate hosts, vectors, and the environment.<\/jats:p>","DOI":"10.3390\/v16071050","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T03:38:27Z","timestamp":1719805107000},"page":"1050","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First Isolation and Molecular Characterization of Umatilla Virus (Sedoreoviridae, Orbivirus) in Brazil"],"prefix":"10.3390","volume":"16","author":[{"given":"Landeson","family":"Barros","sequence":"first","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3473-1296","authenticated-orcid":false,"given":"Ana L\u00facia","family":"Wanzeller","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8385-8253","authenticated-orcid":false,"given":"Val\u00e9ria","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6667-2796","authenticated-orcid":false,"given":"Jannifer","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-2036","authenticated-orcid":false,"given":"L\u00edvia","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"001782","DOI":"10.1099\/jgv.0.001782","article-title":"ICTV Virus Taxonomy Profile: Sedoreoviridae 2022","volume":"103","author":"Matthijnssens","year":"2022","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.virol.2011.11.020","article-title":"Genetic Characterization of Tribe\u010d Virus and Kemerovo Virus, Two Tick-Transmitted Human-Pathogenic Orbiviruses","volume":"423","author":"Dilcher","year":"2012","journal-title":"Virology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s00705-023-05797-4","article-title":"Changes to Virus Taxonomy and the ICTV Statutes Ratified by the International Committee on Taxonomy of Viruses (2023)","volume":"168","author":"Zerbini","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3409","DOI":"10.1099\/vir.0.81258-0","article-title":"Yunnan orbivirus, a New Orbivirus Species Isolated from Culex tritaeniorhynchus Mosquitoes in China","volume":"86","author":"Attoui","year":"2005","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1098\/rstb.2009.0091","article-title":"Bluetongue in Europe: Past, Present and Future","volume":"364","author":"Wilson","year":"2009","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Belaganahalli, M.N., Maan, S., Maan, N.S., Tesh, R., Attoui, H., and Mertens, P.P.C. (2011). Umatilla Virus Genome Sequencing and Phylogenetic Analysis: Identification of Stretch Lagoon Orbivirus as a New Member of the Umatilla Virus Species. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023605"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1051\/vetres\/2010007","article-title":"Re-Emergence of Bluetongue, African Horse Sickness, and Other Orbivirus Diseases","volume":"41","author":"MacLachlan","year":"2010","journal-title":"Vet. Res."},{"key":"ref_8","unstructured":"Karabatsos, N. (1985). International Catalogue of Arboviruses, Including Certain Other Viruses of Vertebrates, Subcommittee on Information Exchange of the American Committee on Arthropod-borne Viruses by the American Society of Tropical Medicine and Hygiene. [3rd ed.]."},{"key":"ref_9","unstructured":"ICMBio (2014). Relat\u00f3rio Anual de Rotas e \u00c1reas de Concentra\u00e7\u00e3o de Aves Migrat\u00f3rias No Brasil, Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade."},{"key":"ref_10","unstructured":"Martins, F.D., Castilho, A.F., Campos, J., Hatano, F.M., and Rolim, S.G. (2012). Fauna da Floresta Nacional de Caraj\u00e1s, Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"63","DOI":"10.53393\/rial.1993.53.36056","article-title":"Growth and Maintenance of Aedes albopictus Cell Line, Clone C6\/36, in Diferrent Media","volume":"53","author":"Barbosa","year":"1993","journal-title":"Rev. Inst. Adolfo Lutz"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1099\/0022-1317-40-3-531","article-title":"Isolation of a Singh\u2019s Aedes Albopictus Cell Clone Sensitive to Dengue and Chikungunya Viruses","volume":"40","author":"Igarashi","year":"1978","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1093\/bioinformatics\/bts174","article-title":"IDBA-UD: A de Novo Assembler for Single-Cell and Metagenomic Sequencing Data with Highly Uneven Depth","volume":"28","author":"Peng","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1101\/gr.5969107","article-title":"MEGAN Analysis of Metagenomic Data","volume":"17","author":"Huson","year":"2007","journal-title":"Genome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: Genome-Scale Protein Function Classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1093\/bioinformatics\/bti263","article-title":"ProtTest: Selection of Best-Fit Models of Protein Evolution","volume":"21","author":"Abascal","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/S0022-2496(02)00028-7","article-title":"Tutorial on Maximum Likelihood Estimation","volume":"47","author":"Myung","year":"2003","journal-title":"J. Math. Psychol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML Version 8: A Tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence Limits on Phylogenies: An Approach Using the Bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1099\/vir.0.064691-0","article-title":"Genetic and Biological Characterization of Selected Changuinola Viruses (Reoviridae, Orbivirus) from Brazil","volume":"95","author":"Silva","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1051\/vetres:2004021","article-title":"African Horse Sickness","volume":"35","author":"Mellor","year":"2004","journal-title":"Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Belaganahalli, M.N., Maan, S., Maan, N.S., Nomikou, K., Pritchard, I., Lunt, R., Kirkland, P.D., Attoui, H., Brownlie, J., and Mertens, P.P.C. (2012). Full Genome Sequencing and Genetic Characterization of Eubenangee Viruses Identify Pata Virus as a Distinct Species within the Genus Orbivirus. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0031911"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1099\/0022-1317-68-11-2967","article-title":"Variation in the Bluetongue Virus Neutralization Protein VP2","volume":"68 Pt 11","author":"Fukusho","year":"1987","journal-title":"J. Gen. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1099\/vir.0.82456-0","article-title":"Analysis and Phylogenetic Comparisons of Full-Length VP2 Genes of the 24 Bluetongue Virus Serotypes","volume":"88","author":"Maan","year":"2007","journal-title":"J. Gen. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"84","DOI":"10.2307\/4082979","article-title":"Relationships of Birds and Arboviruses","volume":"83","author":"Stamm","year":"1966","journal-title":"Auk"},{"key":"ref_29","first-page":"69","article-title":"Aves Como Hospedeiras de Arbov\u00edrus Na Amaz\u00f4nia Brasileira","volume":"8","author":"Degallier","year":"1992","journal-title":"Bol. do Mus. Para. Em\u00edlio Goeldi"},{"key":"ref_30","first-page":"671","article-title":"The Potential Role of Migratory Birds in the Transmission of Zoonoses","volume":"44","author":"Georgopoulou","year":"2008","journal-title":"Vet. Ital."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1525\/om.2011.71.1.1","article-title":"Are Wild Birds Important in the Transport of Arthropod-Borne Viruses?","volume":"71","author":"Brown","year":"2011","journal-title":"Ornithol. Monogr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1128\/jvi.68.1.158-169.1994","article-title":"Evolution of Alphaviruses in the Eastern Equine Encephalomyelitis Complex","volume":"68","author":"Weaver","year":"1994","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"454","DOI":"10.3201\/eid1403.070816","article-title":"Molecular Epidemiology of Eastern Equine Encephalitis Virus, New York","volume":"14","author":"Young","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1006\/viro.1996.0203","article-title":"Genetic Conservation of Highlands J Viruses","volume":"218","author":"Cilnis","year":"1996","journal-title":"Virology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1093\/jmedent\/43.6.1112","article-title":"Movement of Dengue Vectors between the Human Modified Environment and an Urban Forest in Rio de Janeiro","volume":"43","author":"Neto","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1093\/jmedent\/20.2.193","article-title":"Intercanyon Movement of Marked Culex tarsalis (Diptera: Culicidae)1","volume":"20","author":"Milby","year":"1983","journal-title":"J. Med. Entomol."},{"key":"ref_37","unstructured":"Reisen, W.K., and Reeves, W.C. (1990). Bionomics and Ecology of Culex tarsalis and Other Potential Mosquito Vector Species. Epidemiology and Control of Mosquito Borne Arboviruses in California, 1943\u20131987, California Mosquito & Vector Control Association."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1093\/jmedent\/43.2.344","article-title":"Overwintering of West Nile Virus in Southern California","volume":"43","author":"Reisen","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3201\/eid1303.060301","article-title":"Bird Migration Routes and Risk for Pathogen Dispersion into Western Mediterranean Wetlands","volume":"13","author":"Jourdain","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1126\/science.1166664","article-title":"Tracking Long-Distance Songbird Migration by Using Geolocators","volume":"323","author":"Stutchbury","year":"2009","journal-title":"Science"},{"key":"ref_41","unstructured":"ICMBio (2019). Relat\u00f3rio de Rotas e \u00c1reas de Concentra\u00e7\u00e3o de Aves Migrat\u00f3rias No Brasil, Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s10393-006-0025-9","article-title":"Migrating Birds as Dispersal Vehicles for West Nile Virus","volume":"3","author":"Owen","year":"2006","journal-title":"Ecohealth"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e1941","DOI":"10.1002\/rmv.1941","article-title":"Impact of Transmission Cycles and Vector Competence on Global Expansion and Emergence of Arboviruses","volume":"27","author":"Agarwal","year":"2017","journal-title":"Rev. Med. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1099\/jgv.0.001219","article-title":"Skunk River Virus, a Novel Orbivirus Isolated from Aedes trivittatus in the United States","volume":"100","author":"Tangudu","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_45","unstructured":"Oliveira, C.F. (2018). Relat\u00f3rio de Gest\u00e3o do Exerc\u00edcio 2017, Instituto Evandro Chagas."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/7\/1050\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:07:26Z","timestamp":1760108846000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/7\/1050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,28]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["v16071050"],"URL":"https:\/\/doi.org\/10.3390\/v16071050","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,28]]}}}