{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:14:22Z","timestamp":1760145262619,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jules Verne University of Picardy (Amiens, France) and Amiens University Medical Center (Amiens, France)"},{"name":"French State, Hauts-de-France region, INSERM and A2U Alliance\u2019s Universities"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The BK polyomavirus (BKPyV) is a small DNA non-enveloped virus whose infection is asymptomatic in most of the world\u2019s adult population. However, in cases of immunosuppression, the reactivation of the virus can cause various complications, and in particular, nephropathies in kidney transplant recipients or hemorrhagic cystitis in bone marrow transplant recipients. Recently, it was demonstrated that BKPyV virions can use extracellular vesicles to collectively traffic in and out of cells, thus exiting producing cells without cell lysis and entering target cells by diversified entry routes. By a comparison to other naked viruses, we investigated the possibility that BKPyV virions recruit the Endosomal-Sorting Complexes Required for Transport (ESCRT) machinery through late domains in order to hijack extracellular vesicles. We identified a single potential late domain in the BKPyV structural proteins, a YPX3L motif in the VP1 protein, and used pseudovirions to study the effect of point mutations found in a BKPyV clinical isolate or known to ablate the interaction of such a domain with the ESCRT machinery. Our results suggest that this domain is not involved in BKPyV association with extracellular vesicles but is crucial for capsomere interaction and thus viral particle assembly.<\/jats:p>","DOI":"10.3390\/v16071124","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T08:35:17Z","timestamp":1721032517000},"page":"1124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Conserved YPX3L Motif in the BK Polyomavirus VP1 Protein Is Important for Viral Particle Assembly but Not for Its Secretion into Extracellular Vesicles"],"prefix":"10.3390","volume":"16","author":[{"given":"Marine","family":"Bentz","sequence":"first","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louison","family":"Collet","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-5223","authenticated-orcid":false,"given":"Virginie","family":"Morel","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"},{"name":"Laboratoire de Virologie, Centre Hospitalier Universitaire, 80054 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0310-4150","authenticated-orcid":false,"given":"V\u00e9ronique","family":"Descamps","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"},{"name":"Laboratoire de Virologie, Centre Hospitalier Universitaire, 80054 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9541-5190","authenticated-orcid":false,"given":"Emmanuelle","family":"Blanchard","sequence":"additional","affiliation":[{"name":"INSERM U1259, Universit\u00e9 de Tours et CHU de Tours, 37032 Tours, France"},{"name":"Plateforme IBiSA de Microscopie Electronique, Universit\u00e9 de Tours et CHU de Tours, 37032 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Lambert","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5456-6654","authenticated-orcid":false,"given":"Baptiste","family":"Demey","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"},{"name":"Laboratoire de Virologie, Centre Hospitalier Universitaire, 80054 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etienne","family":"Brochot","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"},{"name":"Laboratoire de Virologie, Centre Hospitalier Universitaire, 80054 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6884-2456","authenticated-orcid":false,"given":"Francois","family":"Helle","sequence":"additional","affiliation":[{"name":"UR UPJV4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie (AGIR), Centre Universitaire de Recherche en Sant\u00e9, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1111\/apm.12134","article-title":"The human polyomavirus BK (BKPyV): Virological background and clinical implications","volume":"121","author":"Rinaldo","year":"2013","journal-title":"Apmis"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Helle, F., Brochot, E., Handala, L., Martin, E., Castelain, S., Francois, C., and Duverlie, G. 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