{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:22:51Z","timestamp":1789514571964,"version":"build-2803163510"},"reference-count":31,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Shenzhen Science and Technology Innovation Committee","doi-asserted-by":"publisher","award":["JCYJ20210324131802008"],"award-info":[{"award-number":["JCYJ20210324131802008"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Shenzhen Science and Technology Innovation Committee","doi-asserted-by":"publisher","award":["HZQB-KCZYB-2020056"],"award-info":[{"award-number":["HZQB-KCZYB-2020056"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen-Hong Kong Cooperation Zone for Technology and Innovation","award":["JCYJ20210324131802008"],"award-info":[{"award-number":["JCYJ20210324131802008"]}]},{"name":"Shenzhen-Hong Kong Cooperation Zone for Technology and Innovation","award":["HZQB-KCZYB-2020056"],"award-info":[{"award-number":["HZQB-KCZYB-2020056"]}]},{"name":"Kobilka Institute of Innovative Drug Discovery and Presidential Fellowship and University Development Fund at the Chinese University of Hong Kong, Shenzhen","award":["JCYJ20210324131802008"],"award-info":[{"award-number":["JCYJ20210324131802008"]}]},{"name":"Kobilka Institute of Innovative Drug Discovery and Presidential Fellowship and University Development Fund at the Chinese University of Hong Kong, Shenzhen","award":["HZQB-KCZYB-2020056"],"award-info":[{"award-number":["HZQB-KCZYB-2020056"]}]},{"name":"Presidential Fellowship at the Chinese University of Hong Kong, Shenzhen","award":["JCYJ20210324131802008"],"award-info":[{"award-number":["JCYJ20210324131802008"]}]},{"name":"Presidential Fellowship at the Chinese University of Hong Kong, Shenzhen","award":["HZQB-KCZYB-2020056"],"award-info":[{"award-number":["HZQB-KCZYB-2020056"]}]},{"name":"Ganghong Young Scholar Development Fund","award":["JCYJ20210324131802008"],"award-info":[{"award-number":["JCYJ20210324131802008"]}]},{"name":"Ganghong Young Scholar Development Fund","award":["HZQB-KCZYB-2020056"],"award-info":[{"award-number":["HZQB-KCZYB-2020056"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Yellow fever virus (YFV) infections can cause severe diseases in humans, resulting in mass casualties in Africa and the Americas each year. Secretory NS1 (sNS1) is thought to be used as a diagnostic marker of flavivirus infections, playing an essential role in the flavivirus life cycle, but little is known about the composition and structure of YFV sNS1. Here, we present that the recombinant YFV sNS1 exists in a heterogeneous mixture of oligomerizations, predominantly in the tetrameric form. The cryoEM structures show that the YFV tetramer of sNS1 is stacked by the hydrophobic interaction between \u03b2-roll domains and greasy fingers. According to the 3D variability analysis, the tetramer is in a semi-stable state that may contain multiple conformations with dynamic changes. We believe that our study provides critical insights into the oligomerization of NS1 and will aid the development of NS1-based diagnoses and therapies.<\/jats:p>","DOI":"10.3390\/v16081212","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T12:27:43Z","timestamp":1722256063000},"page":"1212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Structural Insights into the Dynamic Assembly of a YFV sNS1 Tetramer"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6580-2731","authenticated-orcid":false,"given":"Qi","family":"Pan","sequence":"first","affiliation":[{"name":"Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0467-4163","authenticated-orcid":false,"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5249-2276","authenticated-orcid":false,"given":"Haizhan","family":"Jiao","sequence":"additional","affiliation":[{"name":"Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangzhou Eighth People\u2019s Hospital, Guangzhou Medical University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3658-272X","authenticated-orcid":false,"given":"Hongli","family":"Hu","sequence":"additional","affiliation":[{"name":"Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.tim.2018.05.012","article-title":"Yellow Fever Virus: Knowledge Gaps Impeding the Fight Against an Old Foe","volume":"26","author":"Douam","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1126\/science.aau8225","article-title":"The reemergence of yellow fever","volume":"361","author":"Barrett","year":"2018","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kleinert, R.D.V., Montoya-Diaz, E., Khera, T., Welsch, K., Tegtmeyer, B., Hoehl, S., Ciesek, S., and Brown, R.J.P. 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