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Autonomous Region Veterinary Biological Products Engineering Technology Research Center","award":["210401"],"award-info":[{"award-number":["210401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Salmonella Typhimurium (S. Typhimurium) contamination poses a significant challenge to breeder egg hatchability and chick health, necessitating the exploration of alternative disinfection methods. This study investigates the potential of phage vB_SPuM_SP02 (SP02) as a novel disinfectant for breeder eggs contaminated with S. Typhimurium SM022. Phage SP02 was isolated from poultry farm effluent and characterized for morphology, biological properties, and genome properties. Experimental groups of specific pathogen-free (SPF) eggs were treated with Salmonella and phage SP02, and efficacy was assessed through hatching rates, chick survival, weight, Salmonella load, immune organ indices, and intestinal flora. Phage treatment effectively eradicated Salmonella contamination on eggshells within 12 h, resulting in increased hatching and survival rates compared to controls. Furthermore, phage treatment mitigated weight loss and tissue Salmonella load in chicks without causing immune organ damage while reducing Salmonella spp. abundance in the intestinal tract. This study demonstrates the potential of phage SP02 as an eco-friendly and efficient disinfectant for S. Typhimurium-contaminated breeder eggs, offering promising prospects for practical application in poultry production.<\/jats:p>","DOI":"10.3390\/v16081338","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T03:07:38Z","timestamp":1724296058000},"page":"1338","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of Phage Spray on Hatchability and Chick Quality of Eggs Contaminated with Salmonella Typhimurium"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0986-4476","authenticated-orcid":false,"given":"Leping","family":"Wang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China"},{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinting","family":"Dong","sequence":"additional","affiliation":[{"name":"Guangxi Vocational University of Agriculture, Nanning 530009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunping","family":"Tang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiou","family":"Han","sequence":"additional","affiliation":[{"name":"Animal Disease Prevention and Control Center, Guilin 541000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Bai","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China"},{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyan","family":"Yin","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changting","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxia","family":"Ma","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China"},{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Teng","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Gong","sequence":"additional","affiliation":[{"name":"Animal Science and Technology Station of Guizhou, Guiyang 550018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9619-5628","authenticated-orcid":false,"given":"Hao","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0355-6859","authenticated-orcid":false,"given":"Xiaoye","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1637\/7286-100404R","article-title":"Use of bacteriophages in combination with competitive exclusion to reduce Salmonella from infected chickens","volume":"49","author":"Toro","year":"2005","journal-title":"Avian. 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