{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T20:15:35Z","timestamp":1792959335110,"version":"4.5.0"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Science Research Program of the National Research Foundation (NRF)","award":["2019R1A6A1A03033084"],"award-info":[{"award-number":["2019R1A6A1A03033084"]}]},{"name":"Basic Science Research Program of the National Research Foundation (NRF)","award":["Z-1543069-2023-25-01"],"award-info":[{"award-number":["Z-1543069-2023-25-01"]}]},{"name":"Animal and Plant Quarantine Agency, Republic of Korea","award":["2019R1A6A1A03033084"],"award-info":[{"award-number":["2019R1A6A1A03033084"]}]},{"name":"Animal and Plant Quarantine Agency, Republic of Korea","award":["Z-1543069-2023-25-01"],"award-info":[{"award-number":["Z-1543069-2023-25-01"]}]},{"name":"BK21 FOUR Program funded by Jeonbuk National University Research Grant","award":["2019R1A6A1A03033084"],"award-info":[{"award-number":["2019R1A6A1A03033084"]}]},{"name":"BK21 FOUR Program funded by Jeonbuk National University Research Grant","award":["Z-1543069-2023-25-01"],"award-info":[{"award-number":["Z-1543069-2023-25-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Recently, neurological diseases associated with astroviruses (AstVs) have been reported in pigs, ruminants, minks, and humans. In 2017, neuro-invasive porcine astrovirus (Ni-PAstV) 3 was detected in the central nervous system (CNS) of pigs with encephalomyelitis in Hungary and the USA. In the process of diagnosing domestic pigs exhibiting neurological signs, histopathologic lesions of non-suppurative encephalomyelitis with meningitis, neuronal vacuolation, and gliosis were detected, and PAstV was identified using reverse transcriptase PCR in CNS samples of four pigs in three farms from August to September in 2020, South Korea. Subsequently, the ORF2 region was successfully acquired from three brain samples, facilitating subsequent analysis. Four genotypes of PAstV (PAstV1, 3, 4, and 5) were detected, and coinfection of PAstV with multiple genotypes was observed in brain samples. This is the first study to report Ni-PAstV infection in pigs in South Korea.<\/jats:p>","DOI":"10.3390\/v16091372","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T11:54:10Z","timestamp":1724846050000},"page":"1372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Porcine Astrovirus Infection in Brains of Pigs in Korea"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5808-6594","authenticated-orcid":false,"given":"Jun-Soo","family":"Park","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Gi","family":"Jeong","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6733-9047","authenticated-orcid":false,"given":"Su-Beom","family":"Chae","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myeon-Sik","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Companion and Laboratory Animal Science, Kongju National University, Yesan-eup, Yesan 32439, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6540-8945","authenticated-orcid":false,"given":"Byungkwan","family":"Oh","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology, Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sook-Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1598-4343","authenticated-orcid":false,"given":"Jae-Ku","family":"Oem","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Guix, S., Bosch, A., and Pint\u00f3, R.M. (2012). Astrovirus taxonomy. Astrovirus Research, Springer.","DOI":"10.1007\/978-1-4614-4735-1_6"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1128\/CMR.00013-14","article-title":"Human astroviruses","volume":"27","author":"Bosch","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1099\/vir.0.048744-0","article-title":"Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types","volume":"94","author":"Xiao","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1007\/s00705-011-1088-7","article-title":"Detection and genetic characterization of a novel pig astrovirus: Relationship to other astroviruses","volume":"156","author":"Laurin","year":"2011","journal-title":"Arch. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.vetmic.2010.11.026","article-title":"Multiple novel and prevalent astroviruses in pigs","volume":"149","author":"Luo","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Donato, C., and Vijaykrishna, D. (2017). The broad host range and genetic diversity of mammalian and avian astroviruses. Viruses, 9.","DOI":"10.3390\/v9050102"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/S0140-6736(75)90858-2","article-title":"28 nm particles in faeces in infantile gastroenteritis","volume":"306","author":"Madeley","year":"1975","journal-title":"Lancet"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1128\/JCM.37.9.3055-3058.1999","article-title":"Prevalence of group A rotavirus, human calicivirus, astrovirus, and adenovirus type 40 and 41 infections among children with acute gastroenteritis in Dijon, France","volume":"37","author":"Bon","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_9","first-page":"431","article-title":"A molecular study on the prevalence and coinfections of Rotavirus, Norovirus, Astrovirus and Adenovirus in children with gastroenteritis","volume":"71","author":"Aydin","year":"2019","journal-title":"Minerva Pediatr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.meegid.2018.11.006","article-title":"Changing pattern of prevalence and genetic diversity of rotavirus, norovirus, astrovirus, and bocavirus associated with childhood diarrhea in Asian Russia, 2009\u20132012","volume":"67","author":"Zhirakovskaia","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13126","DOI":"10.1128\/JVI.02426-12","article-title":"Complete genome sequence of a newly identified porcine astrovirus genotype 3 strain US-MO123","volume":"86","author":"Xiao","year":"2012","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.meegid.2011.07.024","article-title":"Astrovirus infections in humans and animals\u2013molecular biology, genetic diversity, and interspecies transmissions","volume":"11","author":"Burnham","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Janowski, A.B. (2021). Beyond the Gastrointestinal Tract: The Emerging and Diverse Tissue Tropisms of Astroviruses. Viruses, 13.","DOI":"10.3390\/v13050732"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1136\/vr.114.11.279-a","article-title":"Astrovirus-like particles associated with hepatitis in ducklings","volume":"114","author":"Gough","year":"1984","journal-title":"Vet. Rec."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8487","DOI":"10.1128\/JVI.74.18.8487-8493.2000","article-title":"Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA","volume":"74","author":"Imada","year":"2000","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"918","DOI":"10.3201\/eid1606.091536","article-title":"Astrovirus encephalitis in boy with X-linked agammaglobulinemia","volume":"16","author":"Quan","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1093\/cid\/ciu940","article-title":"Astrovirus VA1\/HMO-C: An increasingly recognized neurotropic pathogen in immunocompromised patients","volume":"60","author":"Brown","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.vetmic.2015.10.019","article-title":"Molecular investigations on the prevalence and viral load of enteric viruses in pigs from five European countries","volume":"182","author":"Zhou","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1128\/JCM.01040-10","article-title":"Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome by use of viral metagenomics","volume":"48","author":"Hammer","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.3201\/eid1909.130682","article-title":"Divergent astrovirus associated with neurologic disease in cattle","volume":"19","author":"Li","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1111\/tbed.12623","article-title":"A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep","volume":"64","author":"Pfaff","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.3201\/eid2312.170703","article-title":"Porcine astrovirus type 3 in central nervous system of swine with polioencephalomyelitis","volume":"23","author":"Hensch","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.3201\/eid2312.170804","article-title":"Outbreaks of neuroinvasive astrovirus associated with encephalomyelitis, weakness, and paralysis among weaned pigs, Hungary","volume":"23","author":"Boros","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13028-019-0466-0","article-title":"Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): A surprise from the archives","volume":"61","author":"Boujon","year":"2019","journal-title":"Acta Vet. Scand."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"K\u00fcchler, L., Koch, M.C., Seuberlich, T., and Boujon, C.L. (2019). Archive mining brings to light a 25-year old astrovirus encephalitis case in a sheep. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00051"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lee, S.-Y., Kim, J.-H., Kim, Y.-J., Kim, Y.-S., Roh, S.-G., Lee, K.-H., Kim, H.-J., Shin, J.-H., and Oem, J.-K. (2021). Astrovirus infection in cattle with nonsuppurative meningoencephalitis in South Korea. Viruses, 13.","DOI":"10.3390\/v13101941"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chae, S.B., Jeong, C.G., Park, J.S., Na, E.J., and Oem, J.K. (2023). Detection and Genetic Characterization of Astroviruses in Brain Tissues of Wild Raccoon Dogs. Viruses, 15.","DOI":"10.20944\/preprints202306.0265.v1"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wildi, N., and Seuberlich, T. (2021). Neurotropic Astroviruses in Animals. Viruses, 13.","DOI":"10.3390\/v13071201"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.meegid.2019.01.009","article-title":"Astrovirus evolution and emergence","volume":"69","author":"Wohlgemuth","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13028-014-0082-y","article-title":"Astrovirus as a possible cause of congenital tremor type AII in piglets?","volume":"56","author":"Ley","year":"2014","journal-title":"Acta Vet. Scand."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.vetmic.2006.06.020","article-title":"Isolation and partial characterization of a novel porcine astrovirus","volume":"117","author":"Indik","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1007\/s00705-016-2937-1","article-title":"Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease","volume":"161","author":"Padmanabhan","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jcpa.2020.09.014","article-title":"Porcine astrovirus type 5-associated enteritis in pigs","volume":"181","author":"Opriessnig","year":"2020","journal-title":"J. Comp. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Fang, Q., Wang, C., Liu, H., Wu, Q., Liang, S., Cen, M., Dong, Q., Wei, Y., Chen, Y., and Ouyang, K. (2019). Pathogenic characteristics of a porcine astrovirus strain isolated in China. Viruses, 11.","DOI":"10.3390\/v11121156"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rawal, G., Ferreyra, F.M., Macedo, N.R., Bradner, L.K., Harmon, K.M., Mueller, A., Allison, G., Linhares, D.C., and Arruda, B.L. (2019). Detection and cellular tropism of porcine astrovirus type 3 on breeding farms. Viruses, 11.","DOI":"10.3390\/v11111051"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1177\/0300985819875741","article-title":"Polioencephalomyelitis in domestic swine associated with porcine astrovirus type 3","volume":"57","author":"Bradner","year":"2020","journal-title":"Vet. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1177\/03009858211025794","article-title":"Experimental porcine astrovirus type 3-associated polioencephalomyelitis in swine","volume":"58","author":"Ferreyra","year":"2021","journal-title":"Vet. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s11262-012-0816-8","article-title":"Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea","volume":"46","author":"Lee","year":"2013","journal-title":"Virus Genes"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1007\/s00705-015-2436-9","article-title":"Complete genome sequence of a porcine astrovirus from South Korea","volume":"160","author":"Lee","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1007\/s00705-021-05164-1","article-title":"Phylogenetic analysis of kobuviruses and astroviruses from Korean wild boars: 2016\u20132018","volume":"166","author":"Shin","year":"2021","journal-title":"Arch. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9107","DOI":"10.1128\/JVI.00857-08","article-title":"Novel astroviruses in insectivorous bats","volume":"82","author":"Chu","year":"2008","journal-title":"J. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1007\/s00705-018-3992-6","article-title":"Genetic diversity and phylogenetic analysis of newly discovered bat astroviruses in Korea","volume":"163","author":"Lee","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_43","first-page":"60","article-title":"BioEdit: An important software for molecular biology","volume":"2","author":"Hall","year":"2011","journal-title":"GERF Bull Biosci"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3.13.1","DOI":"10.1002\/0471250953.bi0313s48","article-title":"Clustal omega","volume":"48","author":"Sievers","year":"2014","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35.","DOI":"10.1093\/molbev\/msy096"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/sj.mt.6300001","article-title":"Development of a conditionally replicating pseudorabies virus for HER-2\/neu-overexpressing bladder cancer therapy","volume":"15","author":"Shiau","year":"2007","journal-title":"Mol. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0378-1135(00)00141-3","article-title":"Genetic typing of classical swine fever virus","volume":"73","author":"Paton","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jviromet.2012.03.034","article-title":"Development of multiplex PCR for simultaneous detection of six swine DNA and RNA viruses","volume":"183","author":"Xu","year":"2012","journal-title":"J. Virol. Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/irv.12407","article-title":"Rapid detection and subtyping of European swine influenza viruses in porcine clinical samples by haemagglutinin-and neuraminidase-specific tetra-and triplex real-time RT-PCR s","volume":"10","author":"Henritzi","year":"2016","journal-title":"Influenza Other Respir. Viruses"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.1128\/JCM.41.7.3372-3375.2003","article-title":"Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces","volume":"41","author":"La","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/j.1472-765X.2005.01831.x","article-title":"Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces","volume":"42","author":"La","year":"2006","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_52","first-page":"1090","article-title":"Won-Il Kim and Hur J. Virulence-associated Genes and Antimicrobial Resistance of Escherichia coli Isolated From Post-weaning Piglets with Diarrhea in Korea","volume":"5","author":"Seo","year":"2018","journal-title":"J. Bacteriol. Mycol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetmic.2007.02.018","article-title":"Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US","volume":"123","author":"Zhang","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.vetmic.2006.10.008","article-title":"Development of an improved species specific PCR test for detection of Haemophilus parasuis","volume":"119","author":"Angen","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"231","DOI":"10.4142\/jvs.2005.6.3.231","article-title":"Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction","volume":"6","author":"Suh","year":"2005","journal-title":"J. Vet. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"403","DOI":"10.4315\/0362-028X.JFP-10-355","article-title":"Evaluation of a serotyping scheme using a combination of an antibody-based serogrouping method and a multiplex PCR assay for identifying the major serotypes of Listeria monocytogenes","volume":"74","author":"Burall","year":"2011","journal-title":"J. Food Prot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/j.1365-2672.2006.03079.x","article-title":"Real-time PCR method for Salmonella spp. targeting the stn gene","volume":"102","author":"Moore","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"pdb. prot4986","DOI":"10.1101\/pdb.prot4986","article-title":"Hematoxylin and eosin staining of tissue and cell sections","volume":"2008","author":"Fischer","year":"2008","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e00040-18","DOI":"10.1128\/CMR.00040-18","article-title":"Nonsuppurative (aseptic) meningoencephalomyelitis associated with neurovirulent astrovirus infections in humans and animals","volume":"31","author":"Reuter","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1139\/W10-042","article-title":"Genomic analysis of two ORF2 segments of new porcine astrovirus isolates and their close relationship with human astroviruses","volume":"56","author":"Ulloa","year":"2010","journal-title":"Can. J. Microbiol."},{"key":"ref_61","unstructured":"King, A.M. (2012). Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1177\/104063870401600408","article-title":"Investigations into shaking mink syndrome: An encephalomyelitis of unknown cause in farmed mink (Mustela vison) kits in Scandinavia","volume":"16","author":"Dolores","year":"2004","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3318","DOI":"10.1128\/JCM.01195-14","article-title":"Neurotropic astrovirus in cattle with nonsuppurative encephalitis in Europe","volume":"52","author":"Bouzalas","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"K\u00fcchler, L., R\u00fcfli, I., Koch, M.C., Hierweger, M.M., Kauer, R.V., Boujon, C.L., Hilbe, M., Oevermann, A., Zanolari, P., and Seuberlich, T. (2021). Astrovirus-Associated Polioencephalomyelitis in an Alpaca. Viruses, 13.","DOI":"10.3390\/v13010050"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1023\/A:1005870317757","article-title":"Streptococcus suis: Past and present","volume":"21","author":"Staats","year":"1997","journal-title":"Vet. Res. Commun."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/9\/1372\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:44:32Z","timestamp":1760111072000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/9\/1372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,28]]},"references-count":65,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["v16091372"],"URL":"https:\/\/doi.org\/10.3390\/v16091372","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,28]]}}}