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To identify novel therapeutic treatments for JCPyV infection, receptor agonists\/antagonists identified in a previously published drug screen were evaluated for their antiviral properties. Seven drugs were selected and validated using infectivity assays, and the mechanism of inhibition was further explored for G protein coupled receptor (GPCR)-associated inhibitors due to the role of the GPCR 5-hydroxytryptamine 2 receptors (5-HT2Rs) in JCPyV entry. The inhibitors cetirizine and paroxetine both reduced infection early in the JCPyV infectious cycle. Paroxetine specifically reduced viral internalization through altering the receptor density of 5-HT2CR, inhibiting \u03b2-arrestin recruitment to the receptor, and reducing MAPK signaling through ERK. These findings highlight the potential of receptor signaling and viral entry mechanisms as possible targets for antiviral drug development. Further, this research suggests that FDA-approved receptor agonists\/antagonists currently used to treat other medical conditions could be repurposed into antivirals for the possible treatment of JCPyV infection and the fatal disease PML.<\/jats:p>","DOI":"10.3390\/v16101559","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T03:57:52Z","timestamp":1727755072000},"page":"1559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["GPCR Inhibitors Have Antiviral Properties against JC Polyomavirus Infection"],"prefix":"10.3390","volume":"16","author":[{"given":"Amanda L.","family":"Sandberg","sequence":"first","affiliation":[{"name":"Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avery C. 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Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41582-020-00427-y","article-title":"Progressive multifocal leukoencephalopathy and the spectrum of JC virus-related disease","volume":"17","author":"Cortese","year":"2021","journal-title":"Nat. Rev. Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1002\/ana.24371","article-title":"Persistence and pathogenesis of the neurotropic polyomavirus JC","volume":"77","author":"Wollebo","year":"2015","journal-title":"Ann. 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Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1159\/000311014","article-title":"Progressive multifocal leukoencephalopathy deaths in the USA, 1979-2005","volume":"35","author":"Christensen","year":"2010","journal-title":"Neuroepidemiology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1097\/WCO.0b013e328346be57","article-title":"Immune reconstitution inflammatory syndrome and the central nervous system","volume":"24","author":"Johnson","year":"2011","journal-title":"Curr. Opin. Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1097\/QAI.0b013e31817bec64","article-title":"Influence of HAART on the clinical course of HIV-1-infected patients with progressive multifocal leukoencephalopathy: Results of an observational multicenter study","volume":"49","author":"Falco","year":"2008","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1056\/NEJM199805073381903","article-title":"Failure of cytarabine in progressive multifocal leukoencephalopathy associated with human immunodeficiency virus infection. AIDS Clinical Trials Group 243 Team","volume":"338","author":"Hall","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1097\/QAD.0b013e32830a5043","article-title":"Cidofovir in addition to antiretroviral treatment is not effective for AIDS-associated progressive multifocal leukoencephalopathy: A multicohort analysis","volume":"22","author":"Ammassari","year":"2008","journal-title":"AIDS"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1080\/713831540","article-title":"Topotecan in the treatment of acquired immunodeficiency syndrome-related progressive multifocal leukoencephalopathy","volume":"9","author":"Royal","year":"2003","journal-title":"J. Neurovirol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s40261-016-0433-8","article-title":"Treatment of Progressive Multifocal Leukoencephalopathy With Mirtazapine","volume":"36","author":"Jamilloux","year":"2016","journal-title":"Clin. Drug Investig."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Morris-Love, J., and Atwood, W.J. (2022). Complexities of JC Polyomavirus Receptor-Dependent and -Independent Mechanisms of Infection. Viruses, 14.","DOI":"10.3390\/v14061130"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6364","DOI":"10.1128\/JVI.00489-15","article-title":"The Greater Affinity of JC Polyomavirus Capsid for alpha2,6-Linked Lactoseries Tetrasaccharide c than for Other Sialylated Glycans Is a Major Determinant of Infectivity","volume":"89","author":"Stroh","year":"2015","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.chom.2010.09.004","article-title":"Structure-function analysis of the human JC polyomavirus establishes the LSTc pentasaccharide as a functional receptor motif","volume":"8","author":"Neu","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1126\/science.1103492","article-title":"The human polyomavirus, JCV, uses serotonin receptors to infect cells","volume":"306","author":"Elphick","year":"2004","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1128\/JVI.74.5.2288-2292.2000","article-title":"JC virus enters human glial cells by clathrin-dependent receptor-mediated endocytosis","volume":"74","author":"Pho","year":"2000","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13490","DOI":"10.1128\/JVI.02252-13","article-title":"5-HT2 receptors facilitate JC polyomavirus entry","volume":"87","author":"Assetta","year":"2013","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Mehmood, K., Wilczek, M.P., DuShane, J.K., Parent, M.T., Mayberry, C.L., Wallace, J.N., Levasseur, F.L., Fong, T.M., Hess, S.T., and Maginnis, M.S. (2022). Dynamics and Patterning of 5-Hydroxytryptamine 2 Subtype Receptors in JC Polyomavirus Entry. Viruses, 14.","DOI":"10.3390\/v14122597"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e02139-20","DOI":"10.1128\/JVI.02139-20","article-title":"GRK2 mediates beta-arrestin interactions with 5-HT(2) receptors for JC polyomavirus endocytosis","volume":"95","author":"Mayberry","year":"2021","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e01948-18","DOI":"10.1128\/JVI.01948-18","article-title":"JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by beta-Arrestin","volume":"93","author":"Mayberry","year":"2019","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"110558","DOI":"10.1016\/j.cellsig.2022.110558","article-title":"beta-arrestins and G protein-coupled receptor kinases in viral entry: A graphical review","volume":"102","author":"Maginnis","year":"2023","journal-title":"Cell Signal"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1016\/j.celrep.2019.04.067","article-title":"Genetic and Functional Dissection of the Role of Individual 5-HT(2) Receptors as Entry Receptors for JC Polyomavirus","volume":"27","author":"Assetta","year":"2019","journal-title":"Cell Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.virol.2012.03.014","article-title":"The VP1 subunit of JC polyomavirus recapitulates early events in viral trafficking and is a novel tool to study polyomavirus entry","volume":"428","author":"Nelson","year":"2012","journal-title":"Virology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/ncb3307","article-title":"beta-Arrestin drives MAP kinase signalling from clathrin-coated structures after GPCR dissociation","volume":"18","author":"Eichel","year":"2016","journal-title":"Nat. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e01529-17","DOI":"10.1128\/JVI.01529-17","article-title":"ERK Is a Critical Regulator of JC Polyomavirus Infection","volume":"92","author":"DuShane","year":"2018","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"DuShane, J.K., Mayberry, C.L., Wilczek, M.P., Nichols, S.L., and Maginnis, M.S. (2019). JCPyV-Induced MAPK Signaling Activates Transcription Factors during Infection. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194779"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"DuShane, J.K., Wilczek, M.P., Crocker, M.A., and Maginnis, M.S. (2019). High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00783"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kaiserman, J., O\u2019Hara, B.A., Haley, S.A., and Atwood, W.J. (2023). An Elusive Target: Inhibitors of JC Polyomavirus Infection and Their Development as Therapeutics for the Treatment of Progressive Multifocal Leukoencephalopathy. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24108580"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"105817","DOI":"10.1016\/j.antiviral.2024.105817","article-title":"High-throughput drug screen identifies calcium and calmodulin inhibitors that reduce JCPyV infection","volume":"222","author":"Bond","year":"2024","journal-title":"Antivir. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wilczek, M.P., Armstrong, F.J., Geohegan, R.P., Mayberry, C.L., DuShane, J.K., King, B.L., and Maginnis, M.S. (2021). The MAPK\/ERK Pathway and the Role of DUSP1 in JCPyV Infection of Primary Astrocytes. Viruses, 13.","DOI":"10.3390\/v13091834"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0042-6822(89)90425-X","article-title":"Extension of JC virus host range to monkey cells by insertion of a simian virus 40 enhancer into the JC virus regulatory region","volume":"170","author":"Vacante","year":"1989","journal-title":"Virology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9677","DOI":"10.1128\/JVI.00978-10","article-title":"Role of N-linked glycosylation of the 5-HT2A receptor in JC virus infection","volume":"84","author":"Maginnis","year":"2010","journal-title":"J. Virol."},{"key":"ref_38","first-page":"e50680","article-title":"Simultaneous multicolor imaging of biological structures with fluorescence photoactivation localization microscopy","volume":"82","author":"Curthoys","year":"2013","journal-title":"J. Vis. Exp."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nmeth929","article-title":"Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)","volume":"3","author":"Rust","year":"2006","journal-title":"Nat. Methods"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/nprot.2011.336","article-title":"Direct stochastic optical reconstruction microscopy with standard fluorescent probes","volume":"6","author":"Loschberger","year":"2011","journal-title":"Nat. Protoc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1016\/j.bpj.2011.07.049","article-title":"Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells","volume":"101","author":"Gunewardene","year":"2011","journal-title":"Biophys. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"094011","DOI":"10.1088\/2040-8978\/15\/9\/094011","article-title":"Bleed-through correction for rendering and correlation analysis in multi-colour localization microscopy","volume":"15","author":"Kim","year":"2013","journal-title":"J. Opt."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1016\/j.bpj.2013.03.054","article-title":"Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin","volume":"104","author":"Gudheti","year":"2013","journal-title":"Biophys. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110198","DOI":"10.1016\/j.virol.2024.110198","article-title":"Conserved sequence features in intracellular domains of viral spike proteins","volume":"599","author":"Ngo","year":"2024","journal-title":"Virology"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Wilczek, M.P., Armstrong, F.J., Mayberry, C.L., King, B.L., and Maginnis, M.S. (2021). PI3K\/AKT\/mTOR Signaling Pathway Is Required for JCPyV Infection in Primary Astrocytes. Cells, 10.","DOI":"10.3390\/cells10113218"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s40248-019-0203-6","article-title":"Focus on the cetirizine use in clinical practice: A reappraisal 30 years later","volume":"14","author":"Corsico","year":"2019","journal-title":"Multidiscip. Respir. Med."},{"key":"ref_47","first-page":"CD006531","article-title":"Paroxetine versus other anti-depressive agents for depression","volume":"2014","author":"Purgato","year":"2014","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.bbrc.2020.11.095","article-title":"Identification of antiviral antihistamines for COVID-19 repurposing","volume":"538","author":"Reznikov","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e1109","DOI":"10.1002\/hsr2.1109","article-title":"Covid-19 Histamine theory: Why antihistamines should be incorporated as the basic component in Covid-19 management?","volume":"6","author":"Mashauri","year":"2023","journal-title":"Health Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100140","DOI":"10.1016\/j.medidd.2022.100140","article-title":"Current status of antihistamine drugs repurposing for infectious diseases","volume":"15","author":"Travi","year":"2022","journal-title":"Med. Drug Discov."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3679","DOI":"10.1038\/s41467-018-06119-y","article-title":"Phosphoproteomic-based kinase profiling early in influenza virus infection identifies GRK2 as antiviral drug target","volume":"9","author":"Yanguez","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Kowalska, M., Nowaczyk, J., Fijalkowski, L., and Nowaczyk, A. (2021). Paroxetine-Overview of the Molecular Mechanisms of Action. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22041662"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.neuropharm.2011.01.012","article-title":"Irving Page Lecture: 5-HT(2A) serotonin receptor biology: Interacting proteins, kinases and paradoxical regulation","volume":"61","author":"Roth","year":"2011","journal-title":"Neuropharmacology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e00379-19","DOI":"10.1128\/mBio.00379-19","article-title":"JC Polyomavirus Uses Extracellular Vesicles To Infect Target Cells","volume":"10","author":"Gee","year":"2019","journal-title":"mBio"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2014.11.010","article-title":"A screen of the NIH Clinical Collection small molecule library identifies potential anti-coronavirus drugs","volume":"114","author":"Cao","year":"2015","journal-title":"Antivir. Res."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Kutkat, O., Moatasim, Y., Al-Karmalawy, A.A., Abulkhair, H.S., Gomaa, M.R., El-Taweel, A.N., Abo Shama, N.M., GabAllah, M., Mahmoud, D.B., and Kayali, G. (2022). Robust antiviral activity of commonly prescribed antidepressants against emerging coronaviruses: In vitro and in silico drug repurposing studies. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-17082-6"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1021\/acs.jmedchem.7b01249","article-title":"Target Identification and Mode of Action of Four Chemically Divergent Drugs against Ebolavirus Infection","volume":"61","author":"Ren","year":"2018","journal-title":"J. Med. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e2136510","DOI":"10.1001\/jamanetworkopen.2021.36510","article-title":"Do the Selective Serotonin Reuptake Inhibitor Antidepressants Fluoxetine and Fluvoxamine Reduce Mortality Among Patients With COVID-19?","volume":"4","author":"Hoertel","year":"2021","journal-title":"JAMA Netw. Open"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s40588-017-0069-3","article-title":"JC Polyomavirus Attachment and Entry: Potential Sites for PML Therapeutics","volume":"4","author":"Mayberry","year":"2017","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.virusres.2007.11.002","article-title":"Interferon beta1-a and selective anti-5HT(2a) receptor antagonists inhibit infection of human glial cells by JC virus","volume":"132","author":"Atwood","year":"2008","journal-title":"Virus Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1016\/S0140-6736(20)31022-9","article-title":"Remdesivir in adults with severe COVID-19: A randomised, double-blind, placebo-controlled, multicentre trial","volume":"395","author":"Wang","year":"2020","journal-title":"Lancet"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s11095-022-03179-0","article-title":"Differences in Cerebral Distribution between Imipramine and Paroxetine via Membrane Transporters at the Rat Blood-Brain Barrier","volume":"39","author":"Akanuma","year":"2022","journal-title":"Pharm. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/S0091-6749(05)80248-9","article-title":"Cetirizine: Actions on neurotransmitter receptors","volume":"86","author":"Snowman","year":"1990","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e0104924","DOI":"10.1128\/mbio.01049-24","article-title":"Brincidofovir inhibits polyomavirus infection in vivo","volume":"15","author":"Butic","year":"2024","journal-title":"mBio"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/10\/1559\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:08:10Z","timestamp":1760112490000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/10\/1559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,30]]},"references-count":64,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["v16101559"],"URL":"https:\/\/doi.org\/10.3390\/v16101559","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,30]]}}}