{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:50:24Z","timestamp":1777492224804,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:00Z","timestamp":1729814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["99999.005580\/2014-05"],"award-info":[{"award-number":["99999.005580\/2014-05"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Loreto virus (LORV) is an insect-specific virus classified into the proposed taxon Negevirus. It was originally described in Iquitos, Peru, in 1977. Here, we describe three novel LORV genomes obtained from the isolates of three pooled samples of Trichoprosopon digitatum, Aedes (Ochlerotatus) fulvus, and Limatus durhamii collected in Ilh\u00e9us\u2014Bahia, 2014. Samples were submitted to RNA sequencing on the Illumina platform to recover the LORV genome. The genomes presented, on average, 81.5% nucleotide identity and 92.6% global amino acid identity with the LORV reference genome (NC_034158). Subsequently, phylogenetic analysis was performed based on a multiple sequence alignment of the concatenated amino acid sequences predicted for the three ORFs of the Negevirus genomes, and the target sequences were clustered within the LORV clade. The taxon Negevirus is in constant expansion of its species content and host range. New data about insect specific negeviruses are important for virus evolution studies, along with those approaching interactions with the hosts and their influence in the transmission of arboviruses. Also, the assessment of these data may allow the development of biologic control strategies for arboviral vectors. This is the original report of the identification of LORV in Brazil, infecting three Culicidae species hosts native to the Atlantic Forest biome.<\/jats:p>","DOI":"10.3390\/v16111674","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T04:15:02Z","timestamp":1730088902000},"page":"1674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First Description of Loreto Virus in Three Culicidae Species from the Atlantic Forest, Bahia, Brazil"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7667-4982","authenticated-orcid":false,"given":"Thito Y. Bezerra","family":"da Paz","sequence":"first","affiliation":[{"name":"Parasite Biology in the Amazon Region Graduate Program, Par\u00e1 State University, Bel\u00e9m 66087-670, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3676-2288","authenticated-orcid":false,"given":"Leonardo H. Almeida","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7510-6641","authenticated-orcid":false,"given":"F\u00e1bio Silva da","family":"Silva","sequence":"additional","affiliation":[{"name":"Parasite Biology in the Amazon Region Graduate Program, Par\u00e1 State University, Bel\u00e9m 66087-670, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana C. Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Parasite Biology in the Amazon Region Graduate Program, Par\u00e1 State University, Bel\u00e9m 66087-670, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7185-4538","authenticated-orcid":false,"given":"Sandro Patroca","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hellen Thais","family":"Fuzii","sequence":"additional","affiliation":[{"name":"Tropical Medicine Center, Federal University of Par\u00e1, Bel\u00e9m 66055-240, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janaina M. Vasconcelos","family":"Massafra","sequence":"additional","affiliation":[{"name":"Center for Technological Innovation, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o L. S. G.","family":"Vianez J\u00fanior","sequence":"additional","affiliation":[{"name":"Center for Technological Innovation, Evandro Chagas Institute, Health and Environmental Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2549-3636","authenticated-orcid":false,"given":"Sharon L.","family":"Deem","sequence":"additional","affiliation":[{"name":"Saint Louis Zoo Institute for Conservation Medicine, Saint Louis, MO 63110, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1774-0713","authenticated-orcid":false,"given":"Leonardo de Carvalho","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Sciences, Faculty of Teacher Training, Rio de Janeiro State University (FFP-UERJ), Rio de Janeiro 24435-005, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-5603","authenticated-orcid":false,"given":"Kristel Myriam","family":"De Vleeschouwer","sequence":"additional","affiliation":[{"name":"Centre for Research and Conservation, Royal Zoological Society of Antwerp, 2018 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian Silva","family":"Catenacci","sequence":"additional","affiliation":[{"name":"Saint Louis Zoo Institute for Conservation Medicine, Saint Louis, MO 63110, USA"},{"name":"Departament of Veterinary Morphophisiology, Applied Technologies for Regional Animals (PPGTAIR), Federal University of Piau\u00ed, Teresina 64049-550, PI, Brazil"},{"name":"Animal Health in the Amazon Graduate Program (PPGSAAM), Federal University of Par\u00e1, Castanhal 68746-630, PA, Brazil"},{"name":"Center for Intelligence on Emergent and Neglected Tropical Diseases, Federal University of Piau\u00ed, Teresina 64049-550, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/JVI.00776-12","article-title":"Negevirus: A Proposed New Taxon of Insect-Specific Viruses with Wide Geographic Distribution","volume":"87","author":"Vasilakis","year":"2013","journal-title":"J. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1007\/s00705-020-04810-4","article-title":"A Novel Negevirus Isolated from Aedes Vexans Mosquitoes in Finland","volume":"165","author":"Suvanto","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12985-022-01743-z","article-title":"Negeviruses Isolated from Mosquitoes in the Brazilian Amazon","volume":"19","author":"Martins","year":"2022","journal-title":"Virol. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/BF01901304","article-title":"The Declining Cocoa Economy and the Atlantic Forest of Southern Bahia, Brazil: Conservation Attitudes of Cocoa Planters","volume":"14","author":"Alger","year":"1994","journal-title":"Environmentalist"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1590\/S0100-67622014000200004","article-title":"Agricultural Management in the Una\u2019s Wildlife Refuge: A Perspective of Conservation by Agroforestry","volume":"38","author":"Sollberg","year":"2014","journal-title":"Rev. \u00c1rvore"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Consoli, R.A.G.B., and de Oliveira, R.L. (1994). Principais Mosquitos de Import\u00e2ncia Sanit\u00e1ria no Brasil, Editora FIOCRUZ.","DOI":"10.7476\/9788575412909"},{"key":"ref_7","unstructured":"(2023, January 19). Babraham Bioinformatics\u2014Trim Galore! Available online:. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/trim_galore\/."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1093\/bioinformatics\/bts565","article-title":"CD-HIT: Accelerated for Clustering the next-Generation Sequencing Data","volume":"28","author":"Fu","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1093\/bioinformatics\/bts611","article-title":"SortMeRNA: Fast and Accurate Filtering of Ribosomal RNAs in Metatranscriptomic Data","volume":"28","author":"Kopylova","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1101\/gr.5969107","article-title":"MEGAN Analysis of Metagenomic Data","volume":"17","author":"Huson","year":"2007","journal-title":"Genome Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An Integrated and Extendable Desktop Software Platform for the Organization and Analysis of Sequence Data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s00705-018-04133-5","article-title":"Complete Genome Sequence of a Novel Negevirus Isolated from Culex Tritaeniorhynchus in China","volume":"164","author":"Wang","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"104561","DOI":"10.1016\/j.meegid.2020.104561","article-title":"Insect-Specific Viruses and Arboviruses in Adult Male Culicids from Midwestern Brazil","volume":"85","author":"Filho","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nature20167","article-title":"Redefining the Invertebrate RNA Virosphere","volume":"540","author":"Shi","year":"2016","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.virusres.2017.11.020","article-title":"A Novel Insect-Infecting Virga\/Nege-like Virus Group and Its Pervasive Endogenization into Insect Genomes","volume":"262","author":"Kondo","year":"2019","journal-title":"Virus Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107494","DOI":"10.1016\/j.jip.2020.107494","article-title":"A Novel Positive Single-Stranded RNA Virus from the Crustacean Parasite, Probopyrinella Latreuticola (Peracarida: Isopoda: Bopyridae)","volume":"177","author":"Bojko","year":"2020","journal-title":"J. Invertebr. Pathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"001863","DOI":"10.1099\/jgv.0.001863","article-title":"Structure and Its Transformation of Elliptical Nege-like Virus Tanay Virus","volume":"104","author":"Okamoto","year":"2023","journal-title":"J. Gen. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"001837","DOI":"10.1099\/jgv.0.001837","article-title":"The Role of Sand Flies as Vectors of Viruses Other than Phleboviruses","volume":"104","author":"Jancarova","year":"2023","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1653\/024.101.0414","article-title":"First Record of Limatus Durhamii Theobald (Diptera: Culicidae) in Campeche, Mexico","volume":"101","year":"2018","journal-title":"Fla. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.actatropica.2016.12.036","article-title":"Snail Shells as Larval Habitat of Limatus Durhamii (Diptera: Culicidae) in the Yungas of Argentina","volume":"167","author":"Mangudo","year":"2017","journal-title":"Acta Trop."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1590\/S0074-02762000000100002","article-title":"Ecology of Mosquitoes (Diptera: Culicidae) in Areas of Serra Do Mar State Park, State of S\u00e3o Paulo, Brazil. II\u2014Habitat Distribution","volume":"95","author":"Gentile","year":"2000","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.4269\/ajtmh.16-0167","article-title":"Identification of Blood Meals from Potential Arbovirus Mosquito Vectors in the Peruvian Amazon Basin","volume":"95","author":"Palermo","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1093\/jme\/tjaa100","article-title":"Mosquitoes (Diptera: Culicidae) from the Southwestern Brazilian Amazon: Liberdade and Greg\u00f3rio Rivers","volume":"57","author":"Hutchings","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_26","first-page":"141","article-title":"Trichoprosopon Digitatum\u2014Morphology, Biology, and Potential Medical Importance","volume":"15","author":"Zavortink","year":"1983","journal-title":"Mosq. Syst."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.virol.2015.04.021","article-title":"Negeviruses Found in Multiple Species of Mosquitoes from Southern Portugal: Isolation, Genetic Diversity, and Replication in Insect Cell Culture","volume":"483","author":"Carapeta","year":"2015","journal-title":"Virology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.virol.2021.01.007","article-title":"Insect-Specific Viruses: An Overview and Their Relationship to Arboviruses of Concern to Humans and Animals","volume":"557","author":"Carvalho","year":"2021","journal-title":"Virology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e0043321","DOI":"10.1128\/JVI.00433-21","article-title":"Negeviruses Reduce Replication of Alphaviruses during Coinfection","volume":"95","author":"Patterson","year":"2021","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1186\/s13071-023-05985-w","article-title":"Interaction of Mesonivirus and Negevirus with Arboviruses and the RNAi Response in Culex Tarsalis-Derived Cells","volume":"16","author":"Agboli","year":"2023","journal-title":"Parasites Vectors"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/11\/1674\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:20:53Z","timestamp":1760113253000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/16\/11\/1674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,25]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["v16111674"],"URL":"https:\/\/doi.org\/10.3390\/v16111674","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,25]]}}}