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the association between infections and other diseases, such as hepatitis and COVID-19. This study aims to investigate the role of miRNA serum expression according to laboratory data concerning hepatitis and COVID-19. Seventy individuals recruited in Southern and Southeastern Brazil donated serum samples and were divided into four groups: (i) 20 negative subjects, (ii) 20 presenting hepatitis, (iii) 19 with COVID-19 and (iv) 11 with hepatitis and COVID-19. Three miRNAs (miR-122, miR-143 and miR-223) were evaluated using real-time PCR. Hematological and biochemical markers were also analyzed. MiR-143 and miR-223 were downregulated among the hepatitis\/COVID-19 group (p &lt; 0.05). A positive correlation was observed between miR-223 and lymphocytes. There was a negative correlation between alanine transaminase (ALT) and aspartate transaminase (AST) for miR-143 and miR-223 and gamma-glutamyl transferase (GGT), alkaline phosphatase (AP) and neutrophil\/lymphocyte ratio (NLR) only for miR-223 (p &lt; 0.05). For hepatic fibrosis (FIB-4), miR-122 and miR-143 had a greater association and miR-223 was more associated with a history of vaccination against COVID-19. MicroRNAs 143 and 223 could be useful as biomarkers for hepatitis coinfection with COVID-19.<\/jats:p>","DOI":"10.3390\/v16111734","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T03:57:34Z","timestamp":1730692654000},"page":"1734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Serum microRNA 143 and 223 Gene Expression Profiles as Potential Biomarkers in Individuals with Hepatitis and COVID-19"],"prefix":"10.3390","volume":"16","author":[{"given":"Lucas Lima","family":"da Silva","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Viral Hepatitis, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7919-8915","authenticated-orcid":false,"given":"Luciane Almeida Amado","family":"Leon","sequence":"additional","affiliation":[{"name":"Technological Development Laboratory, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-8697","authenticated-orcid":false,"given":"Otac\u00edlio","family":"da Cruz Moreira","sequence":"additional","affiliation":[{"name":"Molecular Virology and Parasitology Laboratory, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner Luis","family":"da Costa Nunes Pimentel Coelho","sequence":"additional","affiliation":[{"name":"Technological Development Laboratory, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Duarte","family":"da Costa","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Viral Hepatitis, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5422-557X","authenticated-orcid":false,"given":"Claudia Alexandra Pontes","family":"Ivantes","sequence":"additional","affiliation":[{"name":"Gastroenterology, Hepatology and Liver Transplantation Service, Nossa Senhora das Gra\u00e7as, Curitiba 80810-040, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5358-5426","authenticated-orcid":false,"given":"Priscila","family":"Pollo-Flores","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Fluminense Federal University, Niter\u00f3i 24220-000, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lia Laura","family":"Lewis-Ximenez","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Viral Hepatitis, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6314-754X","authenticated-orcid":false,"given":"Vanessa Salete","family":"de Paula","sequence":"additional","affiliation":[{"name":"Molecular Virology and Parasitology Laboratory, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-8969","authenticated-orcid":false,"given":"Livia Melo","family":"Villar","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Viral Hepatitis, Institute Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"656","DOI":"10.3748\/wjg.v29.i4.656","article-title":"World Journal of Gastroenterology COVID-19 and the liver: Are footprints still there?","volume":"29","author":"Gupta","year":"2023","journal-title":"World J. 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