{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:22:06Z","timestamp":1791836526208,"version":"4.3.4"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Scholar program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The hepatitis B virus (HBV) infects approximately 290 million people globally, with chronic infection sustained by persistent viral gene expression. Recent single-cell analyses of HBV viral transcripts have uncovered novel features of HBV transcription and provided fresh insights into its regulation at the single-cell level. In this review, we summarize the latest advancements in understanding HBV viral transcription in individual hepatocytes and highlight emerging technologies that hold promise for future research.<\/jats:p>","DOI":"10.3390\/v16121828","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T09:59:34Z","timestamp":1732528774000},"page":"1828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New Insights on Hepatitis B Virus Viral Transcription in Single Hepatocytes"],"prefix":"10.3390","volume":"16","author":[{"given":"Bo","family":"Peng","sequence":"first","affiliation":[{"name":"National Institute of Biological Sciences, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Pan","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1305-7404","authenticated-orcid":false,"given":"Wenhui","family":"Li","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing 102206, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S112","DOI":"10.1002\/hep.22920","article-title":"Benefits and risks of nucleoside analog therapy for hepatitis B","volume":"49","author":"Dienstag","year":"2009","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1093\/jac\/dkr388","article-title":"Nucleoside\/nucleotide analogues in the treatment of chronic hepatitis B","volume":"66","author":"Fung","year":"2011","journal-title":"J. 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