{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:41:11Z","timestamp":1773384071124,"version":"3.50.1"},"reference-count":67,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T00:00:00Z","timestamp":1732838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Agency for Scientific and Technological Development (CNPq)","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}]},{"name":"National Agency for Scientific and Technological Development (CNPq)","award":["88887.369255\/2019-00"],"award-info":[{"award-number":["88887.369255\/2019-00"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["406360\/2022-7"],"award-info":[{"award-number":["406360\/2022-7"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["88887.369255\/2019-00"],"award-info":[{"award-number":["88887.369255\/2019-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The biting midges Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) is highly relevant to epidemiology and public health, as it includes species that are potential vectors of human and animal arboviruses. The aim of this study was to investigate the presence of RNA viruses in species of the genus Culicoides collected in the Caraj\u00e1s mining complex in the state of Par\u00e1. The biting midges were collected in the municipalities of Cana\u00e3 dos Caraj\u00e1s, Curion\u00f3polis and Marab\u00e1 and morphologically identified. A total of 1139 specimens of seven Culicoides species were grouped into eight pools and subjected to metagenomic analysis. Eight new insect-specific viruses (ISVs) were characterized and assigned to the order Tolivirales, the families Chuviridae, Nodaviridae, Iflaviridae, Mesoniviridae, and Flaviviridae, and the taxon Negevirus. All viruses identified were assigned to clades, families and taxa never reported in Culicoides in Brazil. This study demonstrated that biting midges harbor a significant diversity of RNA viruses, many of which are still unknown, highlighting the importance of studies aiming at virome of these insects.<\/jats:p>","DOI":"10.3390\/v16121862","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T05:02:22Z","timestamp":1732856542000},"page":"1862","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of RNA Viruses in Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) in a Mining Complex in the Southeastern Region of the Brazilian Amazon"],"prefix":"10.3390","volume":"16","author":[{"given":"S\u00e2mia Luzia Sena da","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro Patroca da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-6496","authenticated-orcid":false,"given":"Carine Fortes","family":"Arag\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inoc\u00eancio de Sousa","family":"Gorayeb","sequence":"additional","affiliation":[{"name":"Coordination of Zoology, Entomology, Museu Paraense Em\u00edlio Goeldi, Bel\u00e9m 66077-830, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Damous","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jannifer Oliveira","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-9984","authenticated-orcid":false,"given":"L\u00edvia Medeiros Neves","family":"Casseb","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto","family":"Nunes Neto","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-2036","authenticated-orcid":false,"given":"L\u00edvia Car\u00edcio","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6603-5527","authenticated-orcid":false,"given":"Pedro Fernando da Costa","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Centre for Biological and Health Sciences, Par\u00e1 State University, Bel\u00e9m 66087-662, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lemos, E.R.S.D., Villar, L.M., Leon, L.A.A., Guimar\u00e3es, M.L., Teixeira, S.L.M., and Paula, V.S.D. (2023). Propriedades Gerais Dos V\u00edrus. T\u00f3picos em Virologia, Editora Fiocruz.","DOI":"10.7476\/9786557082119"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Geoghegan, J.L., and Holmes, E.C. (2017). Predicting Virus Emergence amid Evolutionary Noise. Open Biol., 7.","DOI":"10.1098\/rsob.170189"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Lemos, E.R.S.D., Villar, L.M., Leon, L.A.A., Guimar\u00e3es, M.L., Teixeira, S.L.M., and Paula, V.S.D. (2023). Diagn\u00f3stico de Infec\u00e7\u00f5es Virais. T\u00f3picos Em Virologia, Editora Fiocruz.","DOI":"10.7476\/9786557082119"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zuo, K., Gao, W., Wu, Z., Zhang, L., Wang, J., Yuan, X., Li, C., Xiang, Q., Lu, L., and Liu, H. (2024). Evolution of Virology: Science History through Milestones and Technological Advancements. Viruses, 16.","DOI":"10.3390\/v16030374"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"D943","DOI":"10.1093\/nar\/gkab862","article-title":"ZOVER: The Database of Zoonotic and Vector-Borne Viruses","volume":"50","author":"Zhou","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e02985-21","DOI":"10.1128\/mbio.02985-21","article-title":"The Global Virome in One Network (VIRION): An Atlas of Vertebrate-Virus Associations","volume":"13","author":"Carlson","year":"2022","journal-title":"mBio"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/nature19094","article-title":"Uncovering Earth\u2019s Virome","volume":"536","author":"Pavlopoulos","year":"2016","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Arag\u00e3o, C.F., Da Silva, S.P., Do Nascimento, B.L.S., Da Silva, F.S., Nunes Neto, J.P., Pinheiro, V.C.S., and Cruz, A.C.R. (2023). Shotgun Metagenomic Sequencing Reveals Virome Composition of Mosquitoes from a Transition Ecosystem of North-Northeast Brazil. Genes, 14.","DOI":"10.3390\/genes14071443"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.coviro.2021.04.002","article-title":"The Virome of Vector Mosquitoes","volume":"49","author":"Aguiar","year":"2021","journal-title":"Curr. Opin. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Gangopadhayya, A., Lole, K., Ghuge, O., Ramdasi, A., Kamble, A., Roy, D., Thakar, S., Nath, A., Sudeep, A., and Cherian, S. (2024). Metagenomic Analysis of Viromes of Aedes Mosquitoes across India. Viruses, 16.","DOI":"10.3390\/v16010109"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Sick, F., Beer, M., Kampen, H., and Wernike, K. (2019). Culicoides Biting Midges\u2014Underestimated Vectors for Arboviruses of Public Health and Veterinary Importance. Viruses, 11.","DOI":"10.3390\/v11040376"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mullen, G.R., and Murphree, C.S. (2019). Biting Midges (Ceratopogonidae). Medical and Veterinary Entomology, Elsevier.","DOI":"10.1016\/B978-0-12-814043-7.00013-3"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3201\/eid2702.191551","article-title":"Shuni Virus in Cases of Neurologic Disease in Humans, South Africa","volume":"27","author":"Motlou","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Dagnaw, M., Solomon, A., and Dagnew, B. (2024). Serological Prevalence of the Schmallenberg Virus in Domestic and Wild Hosts Worldwide: A Systematic Review and Meta-Analysis. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1371495"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.coviro.2017.10.004","article-title":"Akabane, Aino and Schmallenberg Virus\u2014Where Do We Stand and What Do We Know about the Role of Domestic Ruminant Hosts and Culicoides Vectors in Virus Transmission and Overwintering?","volume":"27","year":"2017","journal-title":"Curr. Opin. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kampen, H., and Werner, D. (2023). Biting Midges (Diptera: Ceratopogonidae) as Vectors of Viruses. Microorganisms, 11.","DOI":"10.3390\/microorganisms11112706"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Modha, S., Hughes, J., Bianco, G., Ferguson, H.M., Helm, B., Tong, L., Wilkie, G.S., Kohl, A., and Schnettler, E. (2019). Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus. Viruses, 11.","DOI":"10.3390\/v11090865"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Liu, L., Shen, Q., Li, N., He, Y., Han, N., Wang, X., Meng, J., Peng, Y., Pan, M., and Jin, Y. (2021). Comparative Viromes of Culicoides and Mosquitoes Reveal Their Consistency and Diversity in Viral Profiles. Brief. Bioinform., 22.","DOI":"10.1093\/bib\/bbaa323"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"172","DOI":"10.4269\/ajtmh.1981.30.172","article-title":"Oropouche Virus: IV. Laboratory Transmission by Culicoides paraensis","volume":"30","author":"Pinheiro","year":"1981","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1126\/science.6800036","article-title":"Transmission of Oropouche Virus from Man to Hamster by the Midge Culicoides paraensis","volume":"215","author":"Pinheiro","year":"1982","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Scachetti, G.C., Forato, J., Claro, I.M., Hua, X., Salgado, B.B., Vieira, A., Simeoni, C.L., Barbosa, A.R.C., Rosa, I.L., and De Souza, G.F. (2024). Reemergence of Oropouche Virus between 2023 and 2024 in Brazil. medRxiv, Epub ahead of Print.","DOI":"10.1101\/2024.07.27.24310296"},{"key":"ref_22","first-page":"68","article-title":"Transmiss\u00e3o vertical do v\u00edrus Oropouche no Brasil e aspectos cl\u00ednicos: Uma revis\u00e3o de literatura","volume":"1","author":"Lemos","year":"2024","journal-title":"J. Med. Biosci. Res."},{"key":"ref_23","unstructured":"(2024, May 07). Brasil. Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade e Ambiente. Minist\u00e9rio da Sa\u00fade Confirma dois \u00d3bitos por Oropouche no Pa\u00eds. Bras\u00edlia: Minist\u00e9rio da Sa\u00fade, Available online: https:\/\/www.gov.br\/saude\/pt-br\/canais-de-atendimento\/sala-de-imprensa\/notas-a-imprensa\/2024\/ministerio-da-saude-confirma-dois-obitos-por-oropouche-no-pais."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1093\/aesa\/61.3.783","article-title":"A Method for Mounting Small Insects on Microscope Slides in Canada Balsam","volume":"61","author":"Wirth","year":"1968","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1093\/bioinformatics\/bts611","article-title":"SortMeRNA: Fast and Accurate Filtering of Ribosomal RNAs in Metatranscriptomic Data","volume":"28","author":"Kopylova","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ondov, B.D., Bergman, N.H., and Phillippy, A.M. (2011). Interactive Metagenomic Visualization in a Web Browser. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-385"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e43","DOI":"10.1002\/imt2.43","article-title":"Complex Heatmap Visualization","volume":"1","author":"Gu","year":"2022","journal-title":"iMeta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e05378","DOI":"10.7554\/eLife.05378","article-title":"Unprecedented Genomic Diversity of RNA Viruses in Arthropods Reveals the Ancestry of Negative-Sense RNA Viruses","volume":"4","author":"Li","year":"2015","journal-title":"eLife"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Huson, D.H., Beier, S., Flade, I., G\u00f3rska, A., El-Hadidi, M., Mitra, S., Ruscheweyh, H.-J., and Tappu, R. (2016). MEGAN Community Edition\u2014Interactive Exploration and Analysis of Large-Scale Microbiome Sequencing Data. PLoS Comput. Biol., 12.","DOI":"10.1371\/journal.pcbi.1004957"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/S0022-2496(02)00028-7","article-title":"Tutorial on Maximum Likelihood Estimation","volume":"47","author":"Myung","year":"2003","journal-title":"J. Math. Psychol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence Limits on Phylogenies: An Approach Using the Bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1186\/s13071-022-05401-9","article-title":"Aedes aegypti and Ae. albopictus Microbiome\/Virome: New Strategies for Controlling Arboviral Transmission?","volume":"15","author":"Martinez","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100493","DOI":"10.1016\/j.onehlt.2023.100493","article-title":"A Meta-Transcriptomic Study of Mosquito Virome and Blood Feeding Patterns at the Human-Animal-Environment Interface in Guangdong Province, China","volume":"16","author":"Wu","year":"2023","journal-title":"One Health"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Truong Nguyen, P.T., Culverwell, C.L., Suvanto, M.T., Korhonen, E.M., Uusitalo, R., Vapalahti, O., Smura, T., and Huhtamo, E. (2022). Characterisation of the RNA Virome of Nine Ochlerotatus Species in Finland. Viruses, 14.","DOI":"10.3390\/v14071489"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"veab030","DOI":"10.1093\/ve\/veab030","article-title":"Viromics of Extant Insect Orders Unveil the Evolution of the Flavi-like Superfamily","volume":"7","author":"Paraskevopoulou","year":"2021","journal-title":"Virus Evol."},{"key":"ref_39","first-page":"001924","article-title":"ICTV Virus Taxonomy Profile: Jingchuvirales 2023: This Article Is Part of the ICTV Virus Taxonomy Profiles Collection","volume":"104","author":"Kuhn","year":"2023","journal-title":"J. Gen. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Konstantinidis, K., Bampali, M., De Courcy Williams, M., Dovrolis, N., Gatzidou, E., Papazilakis, P., Nearchou, A., Veletza, S., and Karakasiliotis, I. (2022). Dissecting the Species-Specific Virome in Culicoides of Thrace. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.802577"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lara Pinto, A.Z.D., Santos De Carvalho, M., De Melo, F.L., Ribeiro, A.L.M., Morais Ribeiro, B., and Dezengrini Slhessarenko, R. (2017). Novel Viruses in Salivary Glands of Mosquitoes from Sylvatic Cerrado, Midwestern Brazil. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187429"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Maia, L.M.S., Pinto, A.Z.D.L., Carvalho, M.S.D., Melo, F.L.D., Ribeiro, B.M., and Slhessarenko, R.D. (2019). Novel Viruses in Mosquitoes from Brazilian Pantanal. Viruses, 11.","DOI":"10.3390\/v11100957"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"198455","DOI":"10.1016\/j.virusres.2021.198455","article-title":"Metatranscriptomic Analysis Identifies Different Viral-like Sequences in Two Neotropical Mansoniini Mosquito Species","volume":"301","author":"Dezordi","year":"2021","journal-title":"Virus Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1099\/jgv.0.001170","article-title":"ICTV Virus Taxonomy Profile: Nodaviridae","volume":"100","author":"Ninawe","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Miller, L.K., and Ball, L.A. (1998). Nodaviruses of Insects. The Insect Viruses, Springer US.","DOI":"10.1007\/978-1-4615-5341-0"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"225","DOI":"10.7601\/mez.71.225","article-title":"RNA Virome Analysis of Hematophagous Chironomoidea Flies (Diptera: Ceratopogonidae and Simuliidae) Collected in Tokyo, Japan","volume":"71","author":"Kobayashi","year":"2020","journal-title":"Med. Entomol. Zool."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"104561","DOI":"10.1016\/j.meegid.2020.104561","article-title":"Insect-Specific Viruses and Arboviruses in Adult Male Culicids from Midwestern Brazil","volume":"85","author":"Filho","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1099\/jgv.0.000757","article-title":"ICTV Virus Taxonomy Profile: Iflaviridae","volume":"98","author":"Valles","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e00551-21","DOI":"10.1128\/mSphere.00551-21","article-title":"Profiling of RNA Viruses in Biting Midges (Ceratopogonidae) and Related Diptera from Kenya Using Metagenomics and Metabarcoding Analysis","volume":"6","author":"Langat","year":"2021","journal-title":"mSphere"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e02688-22","DOI":"10.1128\/spectrum.02688-22","article-title":"Meta-Viromic Sequencing Reveals Virome Characteristics of Mosquitoes and Culicoides on Zhoushan Island, China","volume":"11","author":"Yang","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ribeiro, G.D.O., Morais, V.S., Monteiro, F.J.C., Ribeiro, E.S.D., Rego, M.O.D.S., Souto, R.N.P., Villanova, F., Tahmasebi, R., Hefford, P.M., and Deng, X. (2020). Aedes Aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species. Viruses, 12.","DOI":"10.3390\/v12080866"},{"key":"ref_52","unstructured":"(2024, May 07). ICTV Fam\u00edlia Mesoniviridae. Available online: https:\/\/ictv.global\/taxonomy."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e00077-11","DOI":"10.1128\/mBio.00077-11","article-title":"An Insect Nidovirus Emerging from a Primary Tropical Rainforest","volume":"2","author":"Zirkel","year":"2011","journal-title":"mBio"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Warrilow, D., Watterson, D., Hall, R.A., Davis, S.S., Weir, R., Kurucz, N., Whelan, P., Allcock, R., Hall-Mendelin, S., and O\u2019Brien, C.A. (2014). A New Species of Mesonivirus from the Northern Territory, Australia. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091103"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e01195-16","DOI":"10.1128\/genomeA.01195-16","article-title":"Novel Viruses Isolated from Mosquitoes in Pantanal, Brazil","volume":"4","author":"Solberg","year":"2016","journal-title":"Genome Announc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.onehlt.2017.02.001","article-title":"Emergence of Wesselsbron Virus among Black Rat and Humans in Eastern Senegal in 2013","volume":"3","author":"Diagne","year":"2017","journal-title":"One Health"},{"key":"ref_57","unstructured":"Braga, C.M. (2022). Metagen\u00f4mica Viral a Partir de D\u00edpteros Hemat\u00f3fagos Coletados No Complexo Minerador de Caraj\u00e1s, Estado Do Par\u00e1. [Ph.D. Thesis, Universidade do Estado do Par\u00e1]."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1099\/jgv.0.000672","article-title":"ICTV Virus Taxonomy Profile: Flaviviridae","volume":"98","author":"Simmonds","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Colmant, A.M.G., Charrel, R.N., and Coutard, B. (2022). Jingmenviruses: Ubiquitous, Understudied, Segmented Flavi-like Viruses. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.997058"},{"key":"ref_60","first-page":"1020","article-title":"Detection of West Nile Virus RNA in Pools of Three Species of Ceratopogonids (Diptera: Ceratopogonidae) Collected in Louisiana","volume":"43","author":"Sabio","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"100490","DOI":"10.1016\/j.onehlt.2023.100490","article-title":"Composition and Global Distribution of the Mosquito Virome\u2014A Comprehensive Database of Insect-Specific Viruses","volume":"16","author":"Moonen","year":"2023","journal-title":"One Health"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Wanzeller, A.L.M., Da Silva, F.S., Hern\u00e1ndez, L.H.A., Barros, L.J.L., Freitas, M.N.O., Santos, M.M., Gon\u00e7alves, E.D.J., Pantoja, J.A.S., Lima, C.D.S., and Lima, M.F. (2023). Isolation of Flaviviruses and Alphaviruses with Encephalitogenic Potential Diagnosed by Evandro Chagas Institute (Par\u00e1, Brazil) in the Period of 1954\u20132022: Six Decades of Discoveries. Viruses, 15.","DOI":"10.3390\/v15040935"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-030-99712-0_1","article-title":"Yellow Fever","volume":"Volume 16","author":"Mehlhorn","year":"2022","journal-title":"Infectious Tropical Diseases and One Health in Latin America"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"S976","DOI":"10.1093\/infdis\/jix405","article-title":"Zika Virus Mosquito Vectors: Competence, Biology, and Vector Control","volume":"216","author":"Kauffman","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e180332","DOI":"10.1590\/0074-02760180332","article-title":"First Isolation of West Nile Virus in Brazil","volume":"114","author":"Martins","year":"2019","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/JVI.00776-12","article-title":"Negevirus: A Proposed New Taxon of Insect-Specific Viruses with Wide Geographic Distribution","volume":"87","author":"Vasilakis","year":"2013","journal-title":"J. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12985-022-01743-z","article-title":"Negeviruses Isolated from Mosquitoes in the Brazilian Amazon","volume":"19","author":"Martins","year":"2022","journal-title":"Virol. 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