{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:34:29Z","timestamp":1784590469016,"version":"3.55.0"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Secretar\u00eda de Investigaci\u00f3n y Posgrado from Instituto Polit\u00e9cnico Nacional","award":["SIP-20242187"],"award-info":[{"award-number":["SIP-20242187"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Chikungunya virus (CHIKV) is classified as a pathogen with the potential to cause a pandemic. This situation becomes more alarming since no approved drug exists to combat the virus. The present research aims to demonstrate the anti-CHIKV activity of molecules present in the latex of Euphorbia grandicornis. Therefore, a biodirected assay was carried out to find the molecules with anti-CHIKV activity. Extractions with hexane, dichloromethane, and methanol and subsequent purification by column chromatography were carried out to later evaluate cytotoxic activity by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and antiviral activity by plaque assay. Our findings show that unlike the others, methanolic extract has a low cytotoxic effect and a good anti-CHIKV effect (EC50 = 26.41 \u00b5g\/mL), which increases when obtaining the purified active fraction (pAFeg1) (EC50 = 0.4835 \u00b5g\/mL). Time-of-addition suggests that the possible mechanism of action of pAFeg1 could be inhibiting any of the non-structural proteins of CHIKV. In addition, both the cytotoxic and anti-CHIKV activity of pAFeg1 demonstrate selectivity since it killed cancer cells and could not inhibit DENV2.<\/jats:p>","DOI":"10.3390\/v16121929","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T08:17:00Z","timestamp":1734423420000},"page":"1929","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Vitro Evaluation of the Anti-Chikungunya Virus Activity of an Active Fraction Obtained from Euphorbia grandicornis Latex"],"prefix":"10.3390","volume":"16","author":[{"given":"Jos\u00e9 Angel","family":"Santiago-Cruz","sequence":"first","affiliation":[{"name":"Laboratorio de Medicina de Conservaci\u00f3n de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9366-3257","authenticated-orcid":false,"given":"Araceli","family":"Posadas-Mondrag\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Medicina de Conservaci\u00f3n de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica","family":"P\u00e9rez-Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Medicina de Conservaci\u00f3n de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma Estela","family":"Herrera-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Oncolog\u00eda Molecular de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Miguel","family":"Chin-Chan","sequence":"additional","affiliation":[{"name":"Laboratorio de Epigen\u00e9tica Ambiental y Salud Mental, Facultad de Ciencias Qu\u00edmico-Biol\u00f3gicas, Universidad Aut\u00f3noma de Campeche, Ciudad de Campeche 24039, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joab Eli","family":"Aguilar-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Medicina de Conservaci\u00f3n de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Leopoldo","family":"Aguilar-Faisal","sequence":"additional","affiliation":[{"name":"Laboratorio de Medicina de Conservaci\u00f3n de la Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.idc.2019.08.006","article-title":"Chikungunya: Epidemiology, Pathogenesis, Clinical Features, Management, and Prevention","volume":"33","author":"Vairo","year":"2019","journal-title":"Infect. 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