{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:15:36Z","timestamp":1787030136304,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation. Widely used cell lines for FMDV and isolation and propagation include baby hamster kidney cells (BHK-21), swine kidney cells (IB-RS-2), foetal goat tongue (ZZ-R 127), foetal porcine kidney cells (LFBKvB6), bovine kidney cells (BK), human telomerase reverse transcriptase bovine thyroid (hTERT-BTY) and porcine kidney-originating PK-15 or SK 6 cell lines. This review highlights how different receptors and molecules\u2014integrins, heparan sulphate (HS), and the Jumonji C-domain containing Protein 6 (JMJD6)\u2014found on the surface of different cell types contribute to differences experienced with susceptibility and sensitivity of the cells to infection with different serotypes of FMDV. This review specifically focuses on Southern African territory (SAT) serotypes, which are unique to the Southern African context and are often under-investigated in cell line development for FMDV isolation and propagation.<\/jats:p>","DOI":"10.3390\/v17010039","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T13:26:25Z","timestamp":1735651585000},"page":"39","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8481-817X","authenticated-orcid":false,"given":"Kitsiso","family":"Gaboiphiwe","sequence":"first","affiliation":[{"name":"World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana"},{"name":"Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tshephang Iris","family":"Kabelo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petronella Thato","family":"Mosholombe","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Hyera","sequence":"additional","affiliation":[{"name":"World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliot Mpolokang","family":"Fana","sequence":"additional","affiliation":[{"name":"World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7128-6999","authenticated-orcid":false,"given":"Kabo","family":"Masisi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8927-091X","authenticated-orcid":false,"given":"Kebaneilwe","family":"Lebani","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"821","DOI":"10.19045\/bspab.2020.90088","article-title":"A review on foot and mouth disease in dairy animals, etiology, pathogenesis and clinical findings","volume":"9","author":"Azeem","year":"2020","journal-title":"Pure Appl. 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