{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:33:35Z","timestamp":1759970015549,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The dengue virus (DENV) is primarily transmitted by Aedes aegypti. Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on the transcriptomic analyses of the midgut and salivary glands from Aedes aegypti infected with DENV2, alongside analyses of Aag2 cell infections, 24 genes potentially related to the regulation of Aedes aegypti infection with DENV2 were selected. By establishing transient transfection and overexpression models of Aedes aegypti Aag2 cells, and mosquito target gene interference models, the difference in viral load before and after treatment was compared, and the effects of DEGs on viral replication were evaluated. After overexpressing 24 DEGs in Aag2 cells, 19 DEGs showed a significant difference in DENV2 RNA copies in the cell supernatant (p &lt; 0.05). In adult mosquitoes, knocking down defensin-A, defensin-A-like, and SMCT1 respectively reduced the DENV2 RNA copies, while knocking down UGT2B1 and ND4 respectively increased the DENV2 RNA copies. In this study, to assess the role of genes related to DENV2 replication, and transient transfection and overexpression models in Aag2 cells and mosquito gene knockdown models were established, and five genes, defensin-A, defensin-A-like, SMCT1, UGT2B1, and ND4, were found to have an impact on the replication of DENV2, providing a reference basis for studying the complex mechanism of mosquito\u2013virus interactions.<\/jats:p>","DOI":"10.3390\/v17010067","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T09:17:02Z","timestamp":1736155022000},"page":"67","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Verification of Differentially Expressed Genes Following DENV2 Infection in Aedes aegypti"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8047-2473","authenticated-orcid":false,"given":"Xiaoli","family":"Chen","sequence":"first","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxue","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-6357","authenticated-orcid":false,"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2143-3350","authenticated-orcid":false,"given":"Teng","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-5743","authenticated-orcid":false,"given":"Haotian","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahong","family":"Wu","sequence":"additional","affiliation":[{"name":"The Key and Characteristic Laboratory of Modern Pathogen Biology, College of Basic Medicine, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxiao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"},{"name":"The Key and Characteristic Laboratory of Modern Pathogen Biology, College of Basic Medicine, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0065-3527(03)59009-1","article-title":"Microevolution and virulence of dengue viruses","volume":"59","year":"2003","journal-title":"Adv. Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6803098","DOI":"10.1155\/2016\/6803098","article-title":"Dengue Fever: Causes, Complications, and Vaccine Strategies","volume":"2016","author":"Khetarpal","year":"2016","journal-title":"J. Immunol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/embo-reports\/kve193","article-title":"Vector-borne diseases and global warming: Are both on an upward swing? Scientists are still debating whether global warming will lead to a further spread of mosquitoes and the diseases they transmit","volume":"2","author":"Brower","year":"2001","journal-title":"EMBO Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/S1473-3099(16)00146-8","article-title":"The global economic burden of dengue: A systematic analysis","volume":"16","author":"Shepard","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/nrmicro1006","article-title":"Transmission cycles, host range, evolution and emergence of arboviral disease","volume":"2","author":"Weaver","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"671","DOI":"10.5811\/westjem.2016.9.30904","article-title":"Dengue, Zika and Chikungunya: Emerging Arboviruses in the New World","volume":"17","author":"Patterson","year":"2016","journal-title":"West. J. Emerg. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.chom.2019.08.005","article-title":"Manipulating Mosquito Tolerance for Arbovirus Control","volume":"26","author":"Lambrechts","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12236","DOI":"10.1128\/JVI.78.22.12236-12242.2004","article-title":"Vesicular stomatitis virus evolution during alternation between persistent infection in insect cells and acute infection in mammalian cells is dominated by the persistence phase","volume":"78","author":"Novella","year":"2004","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4479","DOI":"10.3390\/v6114479","article-title":"Mosquito immunity against arboviruses","volume":"6","author":"Sim","year":"2014","journal-title":"Viruses"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Vargas, I., Scott, J.C., Poole-Smith, B.K., Franz, A.W., Barbosa-Solomieu, V., Wilusz, J., Olson, K.E., and Blair, C.D. (2009). Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito\u2019s RNA interference pathway. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000299"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Xi, Z., Ramirez, J.L., and Dimopoulos, G. (2008). The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000098"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"17841","DOI":"10.1073\/pnas.0905006106","article-title":"An evolutionary conserved function of the JAK-STAT pathway in anti-dengue defense","volume":"106","author":"Sim","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/978-1-4419-8059-5_12","article-title":"Mosquito immunity","volume":"708","author":"Hillyer","year":"2010","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kumar, A., Srivastava, P., Sirisena, P., Dubey, S.K., Kumar, R., Shrinet, J., and Sunil, S. (2018). Mosquito Innate Immunity. Insects, 9.","DOI":"10.3390\/insects9030095"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1186\/1756-3305-7-382","article-title":"Mosquito cell lines: History, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom","volume":"7","author":"Walker","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/211366a0","article-title":"Establishment of a line of mosquito (Aedes aegypti L.) cells grown in vitro","volume":"211","author":"Grace","year":"1966","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/0042-6822(68)90293-6","article-title":"Growth of arboviruses in monolayers from subcultured mosquito embryo cells","volume":"35","author":"Peleg","year":"1968","journal-title":"Virology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"219","DOI":"10.4269\/ajtmh.1968.17.219","article-title":"Growth of arboviruses in primary tissue culture of Aedes aegypti embryos","volume":"17","author":"Peleg","year":"1968","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sim, S., and Dimopoulos, G. (2010). Dengue virus inhibits immune responses in Aedes aegypti cells. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010678"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0965-1748(00)00146-6","article-title":"Exploration of mosquito immunity using cells in culture","volume":"31","author":"Fallon","year":"2001","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.virol.2018.06.007","article-title":"Adventitious viruses persistently infect three commonly used mosquito cell lines","volume":"521","author":"Grubaugh","year":"2018","journal-title":"Virology"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Varjak, M., Donald, C.L., Mottram, T.J., Sreenu, V.B., Merits, A., Maringer, K., Schnettler, E., and Kohl, A. (2017). Characterization of the Zika virus induced small RNA response in Aedes aegypti cells. PLoS Neglected Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0006010"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1046\/j.1365-2583.1999.83119.x","article-title":"Immunity proteins from mosquito cell lines include three defensin A isoforms from Aedes aegypti and a defensin D from Aedes albopictus","volume":"8","author":"Gao","year":"1999","journal-title":"Insect Mol. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/1756-3305-5-148","article-title":"Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies","volume":"5","author":"Barletta","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.4103\/NRR.NRR-D-24-00303","article-title":"Nanocarrier-mediated siRNA delivery: A new approach for the treatment of traumatic brain injury-related Alzheimer\u2019s disease","volume":"20","author":"Jin","year":"2025","journal-title":"Neural Regen. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cois.2019.02.001","article-title":"Novel RNAi delivery systems in the control of medical and veterinary pests","volume":"34","author":"Whitten","year":"2019","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cao, M., Gatehouse, J.A., and Fitches, E.C. (2018). A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041079"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Cruz, C., Tayler, A., and Whyard, S. (2018). RNA Interference-Mediated Knockdown of Male Fertility Genes in the Queensland Fruit Fly Bactrocera tryoni (Diptera: Tephritidae). Insects, 9.","DOI":"10.3390\/insects9030096"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e2301971120","DOI":"10.1073\/pnas.2301971120","article-title":"The sophisticated evolution of Trichoderma to control insect pests","volume":"120","author":"Monte","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","unstructured":"(2024, December 31). State Key Laboratory of Pathogen and Biosecurity, Functional Verification of Related Genes Susceptible to DENV2 in Aedes Aegypti [BioProject ID: PRJNA1197782]. National Center for Biotechnology Information, Available online: https:\/\/www.ncbi.nlm.nih.gov\/sra\/?term=PRJNA1197782."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/s13071-020-04294-w","article-title":"Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection","volume":"13","author":"Li","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijbiomac.2019.12.010","article-title":"The first characterization of a cystatin and a cathepsin L-like peptidase from Aedes aegypti and their possible role in DENV infection by the modulation of apoptosis","volume":"146","author":"Oliveira","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bubner, B., Gase, K., and Baldwin, I.T. (2004). Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR. BMC Biotechnol., 4.","DOI":"10.1186\/1472-6750-4-14"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00894-008-0408-7","article-title":"In-silico homology modeling of three isoforms of insect defensins from the dengue vector mosquito, Aedes aegypti (Linn., 1762)","volume":"15","author":"Dhananjeyan","year":"2009","journal-title":"J. Mol. Model."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8111","DOI":"10.1073\/pnas.1003056107","article-title":"Blocking of Plasmodium transmission by cooperative action of Cecropin A and Defensin A in transgenic Aedes aegypti mosquitoes","volume":"107","author":"Kokoza","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1146\/annurev.immunol.25.022106.141615","article-title":"The host defense of Drosophila melanogaster","volume":"25","author":"Lemaitre","year":"2007","journal-title":"Annu. Rev. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1126\/science.1139862","article-title":"Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes","volume":"316","author":"Waterhouse","year":"2007","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/0965-1748(95)00043-U","article-title":"Insect immunity: Isolation of three novel inducible antibacterial defensins from the vector mosquito, Aedes aegypti","volume":"25","author":"Lowenberger","year":"1995","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_39","first-page":"78","article-title":"Transcription Profiling for Defensins of Aedes aegypti (Diptera: Culicidae) During Development and in Response to Infection with Chikungunya and Zika Viruses","volume":"55","author":"Zhao","year":"2018","journal-title":"J. Med. Entomol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.dmpk.2014.12.001","article-title":"A comprehensive review of UDP-glucuronosyltransferase and esterases for drug development","volume":"30","author":"Oda","year":"2015","journal-title":"Drug Metab. Pharmacokinet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.bbrc.2020.02.075","article-title":"Hetero-oligomer formation of mouse UDP-glucuronosyltransferase (UGT) 2b1 and 1a1 results in the gain of glucuronidation activity towards morphine, an activity which is absent in homo-oligomers of either UGT","volume":"525","author":"Miyauchi","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1124\/dmd.104.000794","article-title":"Drug-drug interactions for UDP-glucuronosyltransferase substrates: A pharmacokinetic explanation for typically observed low exposure (AUCi\/AUC) ratios","volume":"32","author":"Williams","year":"2004","journal-title":"Drug Metab. Dispos. Biol. Fate Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"38","DOI":"10.2174\/1389450118666170125144557","article-title":"Cytochrome P450 Structure, Function and Clinical Significance: A Review","volume":"19","author":"Manikandan","year":"2018","journal-title":"Curr. Drug Targets"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Vontas, J., Katsavou, E., and Mavridis, K. (2020). Cytochrome P450-based metabolic insecticide resistance in Anopheles and Aedes mosquito vectors: Muddying the waters. Pestic. Biochem. Physiol., 170.","DOI":"10.1016\/j.pestbp.2020.104666"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3109\/03602530903208579","article-title":"Modulation of UDP-glucuronosyltransferase activity by protein-protein association","volume":"42","author":"Ishii","year":"2010","journal-title":"Drug Metab. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.devcel.2009.12.013","article-title":"The WW domain protein Kibra acts upstream of Hippo in Drosophila","volume":"18","author":"Baumgartner","year":"2010","journal-title":"Dev. Cell"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Martonosi, A.N. (1985). The Enzymes and the Enzyme Complexes of the Mitochondrial Oxidative Phosphorylation System. The Enzymes of Biological Membranes: Volume 4 Bioenergetics of Electron and Proton Transport, Springer.","DOI":"10.1007\/978-1-4684-4604-3"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/jmedent\/45.3.391","article-title":"Gene flow between domestic and sylvan populations of Aedes aegypti (Diptera: Culicidae) in North Cameroon","volume":"45","author":"Paupy","year":"2008","journal-title":"J. Med. Entomol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1038\/sj.embor.7401091","article-title":"The proteasome maturation protein POMP facilitates major steps of 20S proteasome formation at the endoplasmic reticulum","volume":"8","author":"Fricke","year":"2007","journal-title":"EMBO Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1167\/iovs.06-0888","article-title":"Expression of the sodium-coupled monocarboxylate transporters SMCT1 (SLC5A8) and SMCT2 (SLC5A12) in retina","volume":"48","author":"Martin","year":"2007","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/ki.1986.6","article-title":"Metabolic fuels along the nephron: Pathways and intracellular mechanisms of interaction","volume":"29","author":"Guder","year":"1986","journal-title":"Kidney Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"382","DOI":"10.2337\/diacare.24.2.382","article-title":"Renal gluconeogenesis: Its importance in human glucose homeostasis","volume":"24","author":"Gerich","year":"2001","journal-title":"Diabetes Care"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/ki.1986.8","article-title":"Glucose metabolism in renal tubular function","volume":"29","author":"Ross","year":"1986","journal-title":"Kidney Int."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1042\/BST0330237","article-title":"Biological functions of SLC5A8, a candidate tumour suppressor","volume":"33","author":"Ganapathy","year":"2005","journal-title":"Biochem. Soc. Trans."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1016\/j.lfs.2005.10.028","article-title":"SLC5A8 (SMCT1)-mediated transport of butyrate forms the basis for the tumor suppressive function of the transporter","volume":"78","author":"Gupta","year":"2006","journal-title":"Life Sci."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/1\/67\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:23:28Z","timestamp":1759919008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/1\/67"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["v17010067"],"URL":"https:\/\/doi.org\/10.3390\/v17010067","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2025,1,6]]}}}