{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:00:57Z","timestamp":1791000057075,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shenzhen High-level Hospital Construction Fund","award":["G2022091"],"award-info":[{"award-number":["G2022091"]}]},{"name":"Shenzhen High-level Hospital Construction Fund","award":["XKJS-CRGRK-008"],"award-info":[{"award-number":["XKJS-CRGRK-008"]}]},{"name":"Shenzhen Medical Research Fund","award":["E24010010"],"award-info":[{"award-number":["E24010010"]}]},{"name":"Shenzhen Medical Research Fund","award":["E24010012"],"award-info":[{"award-number":["E24010012"]}]},{"name":"Special Funds for Strategic Emerging Industry of Shenzhen","award":["F-2022-Z99-502266"],"award-info":[{"award-number":["F-2022-Z99-502266"]}]},{"name":"National Key Research and Development Program of China","award":["2023YFC2306400"],"award-info":[{"award-number":["2023YFC2306400"]}]},{"name":"National Key Research and Development Program of China","award":["2023YFC2306401"],"award-info":[{"award-number":["2023YFC2306401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The re-emergence of the mpox pandemic poses considerable challenges to human health and societal development. There is an urgent need for effective prevention and treatment strategies against the mpox virus (MPXV). In this study, we focused on the A35R protein and created a chimeric A35R-Fc protein by fusing the Fc region of IgG to its C-terminal. We then assessed its reactivity with A35R-specific antibodies and human convalescent plasma, as well as its immunogenicity. Our findings indicate that the A35R-Fc protein significantly enhances affinity to A35R antibodies compared to the commercially available A35R protein and exhibits considerable reactivity to human plasma. Additionally, mice immunized with A35R-Fc exhibited increased neutralizing antibody titers against the live MPXV. These results support the potential of Fc domain chimeric antigens as a strategy to enhance the efficacy of subunit vaccines targeting the MPXV.<\/jats:p>","DOI":"10.3390\/v17010116","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T04:55:19Z","timestamp":1737003319000},"page":"116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein"],"prefix":"10.3390","volume":"17","author":[{"given":"Shimeng","family":"Bai","sequence":"first","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"},{"name":"School of Public Health, Bengbu Medical University, Bengbu 233030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxin","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Public Health, Bengbu Medical University, Bengbu 233030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibin","family":"Liao","sequence":"additional","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyang","family":"Yi","sequence":"additional","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghui","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Public Health, Bengbu Medical University, Bengbu 233030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenfang","family":"Wu","sequence":"additional","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhou","family":"Lu","sequence":"additional","affiliation":[{"name":"Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People\u2019s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China"},{"name":"School of Public Health, Bengbu Medical University, Bengbu 233030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1016\/j.cmi.2023.07.011","article-title":"A contemporary review of clade-specific virological differences in monkeypox viruses","volume":"29","author":"Okwor","year":"2023","journal-title":"Clin. 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