{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:10:55Z","timestamp":1780096255542,"version":"3.54.0"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T00:00:00Z","timestamp":1737676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"KNOW-PATH internal activity of the European Partnership on Animal Health and Welfare","award":["HORIZON-CL6-2023-FARM2FORK"],"award-info":[{"award-number":["HORIZON-CL6-2023-FARM2FORK"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The first official case of bluetongue virus serotype 3 (BTV-3) in Portugal was confirmed in sheep from the district of \u00c9vora in September 2024. Notably, mortality was observed in pregnant sheepdogs within the affected sheep flocks. This study presents four cases of pregnant dogs infected with BTV-3 in mid-September 2024, all of which aborted prior to death. BTV-3 was identified by RT-qPCR following initial positive results from pan-BTV RT-qPCR. The virus was subsequently isolated from the blood of one of the dogs in BHK-21 cells, and a partial sequence of the vp2 gene was obtained. This sequence showed 100% similarity to sheep BTV3\/3234\/PT2024, identified in Portugal in September 2024, as well as to BTV-3\/NET2023, first reported in the Netherlands in 2023. These findings suggest that the viruses may be related or share a common origin. Co-infection with common canine viruses and pathogenic bacteria was ruled out, confirming that the fatalities were due to BTV-3 infection, probably by ingestion of sheep placenta after lambing. Our results confirm the potential for the transmission of BTV-3 to non-ruminant species, particularly carnivores, and, therefore, the wider ecological implications of this virus. In addition, the identification of transplacental transmission of BTV-3 in one of the dogs provides new evidence highlighting the complexity of the virus\u2019 transmission mechanisms.<\/jats:p>","DOI":"10.3390\/v17020159","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T04:32:22Z","timestamp":1737693142000},"page":"159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Fatal Bluetongue Virus Serotype 3 Infection in Female Dogs: A Case Report from Alentejo, Portugal, 2024"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6246-1983","authenticated-orcid":false,"given":"S\u00edlvia C.","family":"Barros","sequence":"first","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3445-3035","authenticated-orcid":false,"given":"Diogo","family":"Maroco","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1835-3077","authenticated-orcid":false,"given":"Ana M.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Costa","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio Muralha de \u00c9vora, Rua Marechal Costa Gomes 9, 7005-145 \u00c9vora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Alves","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio Muralha de \u00c9vora, Rua Marechal Costa Gomes 9, 7005-145 \u00c9vora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8561-9846","authenticated-orcid":false,"given":"Fernanda","family":"Ramos","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Duarte","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria, Centre for Interdisciplinary Research in Animal Health (CIISA), Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Avenida da Universidade de Lisboa, 1300-477 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6509-6651","authenticated-orcid":false,"given":"Teresa","family":"Fagulha","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6110-9590","authenticated-orcid":false,"given":"In\u00eas C.","family":"Varanda","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0696-7322","authenticated-orcid":false,"given":"F\u00e1bio Abade dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"CECAV\u2014Centro de Ci\u00eancia Animal e Veterin\u00e1ria, Faculdade de Medicina, Veterin\u00e1ria de Lisboa, Universidade Lus\u00f3fona, Centro Universit\u00e1rio de Lisboa, 1749-024 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9607-7576","authenticated-orcid":false,"given":"Ana C.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"BioISI\u2014Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal"},{"name":"I-MVET, Faculdade de Medicina, Veterin\u00e1ria de Lisboa, Universidade Lus\u00f3fona, Centro Universit\u00e1rio de Lisboa, 1749-024 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Barahona","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Orvalho","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio Muralha de \u00c9vora, Rua Marechal Costa Gomes 9, 7005-145 \u00c9vora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1488-9659","authenticated-orcid":false,"given":"Margarida D.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Nacional Institute of Agrarian and Veterinarian Research, Quinta Do Marqu\u00eas, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria, Centre for Interdisciplinary Research in Animal Health (CIISA), Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Avenida da Universidade de Lisboa, 1300-477 Lisboa, 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