{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:46:29Z","timestamp":1781196389312,"version":"3.54.1"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Nacional de Tecnolog\u00eda Agrope-cuaria (INTA)","award":["PE-I044"],"award-info":[{"award-number":["PE-I044"]}]},{"name":"Instituto Nacional de Tecnolog\u00eda Agrope-cuaria (INTA)","award":["RIST-111"],"award-info":[{"award-number":["RIST-111"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.<\/jats:p>","DOI":"10.3390\/v17020211","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T05:36:32Z","timestamp":1738560992000},"page":"211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis"],"prefix":"10.3390","volume":"17","author":[{"given":"Ricardo Gabriel","family":"D\u00edaz Alarc\u00f3n","sequence":"first","affiliation":[{"name":"Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM), Posadas CP3300, Misiones, Argentina"},{"name":"National Council for Scientific and Technical Research (CONICET), Av. Mariano Moreno 1375, Lab 105, Posadas CP3300, Misiones, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-4776","authenticated-orcid":false,"given":"Karina","family":"Salvatierra","sequence":"additional","affiliation":[{"name":"Laboratory \u201cMADAR\u201d, National University of Misiones (UNaM), Ruta 12, Km 7 y \u00bd, Posadas CP3300, Misiones, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiliano","family":"G\u00f3mez Quintero","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM), Posadas CP3300, Misiones, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4441-0234","authenticated-orcid":false,"given":"Domingo Javier","family":"Liotta","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM), Posadas CP3300, Misiones, Argentina"},{"name":"National Institute of Tropical Medicine (INMeT)\u2014ANLIS \u201cDr. Carlos Malbr\u00e1n\u201d, Puerto Iguaz\u00fa CP3370, Misiones, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-5592","authenticated-orcid":false,"given":"Viviana","family":"Parre\u00f1o","sequence":"additional","affiliation":[{"name":"National Institute of Agricultural Technology (INTA), IncuINTA, De Las Caba\u00f1as y De los Reseros s\/n, Hurlingham CP1816, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1048-8532","authenticated-orcid":false,"given":"Samuel Orlando","family":"Mi\u00f1o","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM), Posadas CP3300, Misiones, Argentina"},{"name":"National Institute of Agricultural Technology (INTA), EEA Cerro Azul, National Route 14, Km 836, Cerro Azul CP3313, Misiones, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.virusres.2014.06.016","article-title":"Rotaviruses","volume":"190","author":"Desselberger","year":"2014","journal-title":"Virus Res."},{"key":"ref_2","unstructured":"Knipe, D.M., and Howley, P.M. (2013). Rotavirus. Fields Virology, Lippincott Williams & Wilkins."},{"key":"ref_3","unstructured":"Fauquet, C., Mayo, M., Maniloff, J., Desselberger, U., and Ball, A. (2011). Genus Rotavirus: Type Species A. Virus Taxonomy: Classification and Nomenclature of Viruses, Elsevier Academic Press."},{"key":"ref_4","unstructured":"Bitton, G. (2002). Rotaviruses: Basic Biology, Epidemiology and Methodologies. Encyclopedia of Environmental Microbiology, John Wiley & Sons."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0890-8508(89)90006-6","article-title":"Identification of Rotavirus Genogroups by RNA-RNA Hybridization","volume":"3","author":"Nakagomi","year":"1989","journal-title":"Mol. Cell. Probes"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3204","DOI":"10.1128\/JVI.02257-07","article-title":"Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains","volume":"82","author":"Matthijnssens","year":"2008","journal-title":"J. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1006\/viro.1996.0586","article-title":"Genetic Stability of a Porcine Rotavirus RNA Segment during Repeated Plaque Isolation","volume":"225","author":"Blackhall","year":"1996","journal-title":"Virology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1099\/jgv.0.000397","article-title":"Genetic Linkage of Capsid Protein-Encoding RNA Segments in Group A Equine Rotaviruses","volume":"97","author":"Barrandeguy","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"D\u00edaz Alarc\u00f3n, R.G., Liotta, D.J., and Mi\u00f1o, S. (2022). Zoonotic RVA: State of the Art and Distribution in the Animal World. Viruses, 14.","DOI":"10.3390\/v14112554"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.vetmic.2009.08.028","article-title":"Zoonotic Aspects of Rotaviruses","volume":"140","author":"Martella","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.jinf.2004.01.018","article-title":"The Zoonotic Potential of Rotavirus","volume":"48","author":"Cook","year":"2004","journal-title":"J. Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5627","DOI":"10.1016\/j.vaccine.2013.08.045","article-title":"Global Distribution of Group A Rotavirus Strains in Horses: A Systematic Review","volume":"31","author":"Papp","year":"2013","journal-title":"Vaccine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.vetmic.2013.03.020","article-title":"Review of Group A Rotavirus Strains Reported in Swine and Cattle","volume":"165","author":"Papp","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s13337-014-0194-z","article-title":"Exotic Rotaviruses in Animals and Rotaviruses in Exotic Animals","volume":"25","author":"Ghosh","year":"2014","journal-title":"Virusdisease"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.meegid.2014.08.017","article-title":"Review of Global Rotavirus Strain Prevalence Data from Six Years Post Vaccine Licensure Surveillance: Is There Evidence of Strain Selection from Vaccine Pressure?","volume":"28","author":"Martella","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sim\u00f3n, D., Cristina, J., and Musto, H. (2021). Nucleotide Composition and Codon Usage Across Viruses and Their Respective Hosts. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.646300"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/s00239-005-0221-1","article-title":"Evolutionary Basis of Codon Usage and Nucleotide Composition Bias in Vertebrate DNA Viruses","volume":"62","author":"Shackelton","year":"2006","journal-title":"J. Mol. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nrg2323","article-title":"Rates of Evolutionary Change in Viruses: Patterns and Determinants","volume":"9","author":"Duffy","year":"2008","journal-title":"Nat. Rev. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9733","DOI":"10.1128\/JVI.00694-10","article-title":"Viral Mutation Rates","volume":"84","author":"Nebot","year":"2010","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Pfeiffer, J.K., and Kirkegaard, K. (2005). Increased Fidelity Reduces Poliovirus Fitness and Virulence under Selective Pressure in Mice. PLoS Pathog., 1.","DOI":"10.1371\/journal.ppat.0010011"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nature04388","article-title":"Quasispecies Diversity Determines Pathogenesis through Cooperative Interactions in a Viral Population","volume":"439","author":"Vignuzzi","year":"2006","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/nm1726","article-title":"Engineering Attenuated Virus Vaccines by Controlling Replication Fidelity","volume":"14","author":"Vignuzzi","year":"2008","journal-title":"Nat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"31618","DOI":"10.1074\/jbc.C112.401471","article-title":"A Polymerase Mechanism-Based Strategy for Viral Attenuation and Vaccine Development","volume":"287","author":"Weeks","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev.micro.58.030603.123649","article-title":"Viral Error Catastrophe by Mutagenic Nucleosides","volume":"58","author":"Anderson","year":"2004","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1146\/annurev.micro.62.081307.162912","article-title":"Evolutionary History and Phylogeography of Human Viruses","volume":"62","author":"Holmes","year":"2008","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1080\/10408410701647560","article-title":"Ecological Origins of Novel Human Pathogens","volume":"33","author":"Woolhouse","year":"2007","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Combe, M., and Sanju\u00e1n, R. (2014). Variation in RNA Virus Mutation Rates across Host Cells. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1003855"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s00705-008-0155-1","article-title":"Recommendations for the Classification of Group A Rotaviruses Using All 11 Genomic RNA Segments","volume":"153","author":"Matthijnssens","year":"2008","journal-title":"Arch. Virol."},{"key":"ref_29","unstructured":"(2024, February 05). Rotavirus Classification Working Group: RCWG Home Page. Newly Assigned Genotypes, List of Accepted Genotypes. Available online: https:\/\/rega.kuleuven.be\/cev\/viralmetagenomics\/virus-classification\/rcwg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lemey, P., Salemi, M., and Vandamme, A.-M. (2009). The Phylogenetic Handbook, Cambridge University Press. [2nd ed.].","DOI":"10.1017\/CBO9780511819049"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1002\/jmv.22137","article-title":"Genotype Diversity of Group A Rotavirus Strains in Children With Acute Diarrhea in Urban Burkina Faso, 2008\u20132010","volume":"83","author":"Bonkoungou","year":"2011","journal-title":"J. Med. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/0042-6822(89)90620-X","article-title":"Rotavirus VP7 Neutralization Epitopes of Serotype 3 Strains","volume":"171","author":"Nlshikawa","year":"1989","journal-title":"Virology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8213","DOI":"10.1128\/jvi.71.11.8213-8220.1997","article-title":"Single Point Mutations May Affect the Serotype Reactivity of Serotype G11 Porcine Rotavirus Strains: A Widening Spectrum?","volume":"71","author":"Ciarlet","year":"1997","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1128\/jcm.32.10.2609-2612.1994","article-title":"Equine Rotaviruses with G14 Serotype Specificity Circulate among Venezuelan Horses","volume":"32","author":"Ciarlet","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11262-017-1529-9","article-title":"Phylogenetic Comparison of the VP7, VP4, VP6, and NSP4 Genes of Rotaviruses Isolated from Children in Nizhny Novgorod, Russia, 2015\u20132016, with Cogent Genes of the Rotarix and RotaTeq Vaccine Strains","volume":"54","author":"Morozova","year":"2018","journal-title":"Virus Genes"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1099\/jgv.0.001323","article-title":"Rotavirus G9P[4], G9P[6] and G1P[6] Strains Isolated from Children with Acute Gastroenteritis in Pune, Western India, 2013\u20132015: Evidence for Recombination in Genes Encoding VP3, VP4 and NSP1","volume":"100","author":"Tatte","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Sircar, S., Malik, Y.S., Kumar, P., Ansari, M.I., Bhat, S., Shanmuganathan, S., Kattoor, J.J., Vinodhkumar, O.R., Rishi, N., and Touil, N. (2021). Genomic Analysis of an Indian G8P[1] Caprine Rotavirus-A Strain Revealing Artiodactyl and DS-1-Like Human Multispecies Reassortment. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.606661"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.vetmic.2015.09.017","article-title":"Whole Genomic Analysis of a Porcine G6P[13] Rotavirus Strain","volume":"180","author":"Ghosh","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.meegid.2017.06.008","article-title":"Detection of Uncommon G3P[3] Rotavirus A (RVA) Strain in Rat Possessing a Human RVA-like VP6 and a Novel NSP2 Genotype","volume":"53","author":"Ianiro","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1007\/s00705-019-04271-4","article-title":"Evidence of Zoonotic Transmission of VP6 and NSP4 Genes into Human Species A Rotaviruses Isolated in Pakistan in 2010","volume":"164","author":"Tamim","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"jgv001532","DOI":"10.1099\/jgv.0.001532","article-title":"Genomic Analysis Reveals G3P[13] Porcine Rotavirus A Interspecific Transmission to Human from Pigs in a Swine Farm with Diarrhoea Outbreak","volume":"102","author":"Yan","year":"2021","journal-title":"J. General. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s12985-023-02064-5","article-title":"Reassortment and Genomic Analysis of a G9P[8]-E2 Rotavirus Isolated in China","volume":"20","author":"Peng","year":"2023","journal-title":"Virol. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/jvi.01408-22","article-title":"Complete Genome Sequencing Reveals Unusual Equine Rotavirus A of Bat Origin from India","volume":"96","author":"Pathak","year":"2022","journal-title":"J. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e317","DOI":"10.1111\/tbed.12756","article-title":"A G3P[13] Porcine Group A Rotavirus Emerging in China Is a Reassortant and a Natural Recombinant in the VP4 Gene","volume":"65","author":"Jing","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/jmv.21968","article-title":"VP7 Gene of Human Rotavirus A Genotype G5: Phylogenetic Analysis Reveals the Existence of Three Different Lineages Worldwide","volume":"83","author":"Tort","year":"2011","journal-title":"J. Med. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6200","DOI":"10.1002\/jmv.27142","article-title":"Phylogenetic Analysis of VP7 and VP4 Genes of the Most Predominant Human Group A Rotavirus G12 Identified in Children with Acute Gastroenteritis in Himachal Pradesh, India during 2013\u20132016","volume":"93","author":"Gupta","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00705-023-05874-8","article-title":"Genetic Diversity of Rotavirus A in Argentina during 2019-2022: Detection of G6 Strains and Insights Regarding Its Dissemination","volume":"168","author":"Degiuseppe","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.meegid.2019.02.007","article-title":"Whole Genome Characterisation of G11P[25] and G9P[19] Rotavirus A Strains from Adult Patients with Diarrhoea in Nepal","volume":"69","author":"Takatsuki","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_50","unstructured":"Fields, B.N., Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., and Straus, S.E. (2007). Rotaviruses and Their Replication. Fields Virology, Lippincott, Williams and Wilkins."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.1016\/S0264-410X(02)00313-4","article-title":"Generation and Characterization of Six Single VP4 Gene Substitution Reassortant Rotavirus Vaccine Candidates: Each Bears a Single Human Rotavirus VP4 Gene Encoding P Serotype 1A[8] or 1B[4] and the Remaining 10 Genes of Rhesus Monkey Rotavirus MMU18006 or Bovine Rotavirus UK","volume":"20","author":"Hoshino","year":"2002","journal-title":"Vaccine"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.1073\/pnas.87.18.7155","article-title":"Antigenic Relationships among Human Rotaviruses as Determined by Outer Capsid Protein VP4 (Rotavirus VP4 Expression\/Rotavirus VP4 Serotypes\/Rotavirus VP8\/Rotavirus VP5)","volume":"87","author":"Gorziglia","year":"1990","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Chen, J., Grow, S., Iturriza-G\u00f3mara, M., Hausdorff, W.P., Fix, A., and Kirkwood, C.D. (2022). The Challenges and Opportunities of Next-Generation Rotavirus Vaccines: Summary of an Expert Meeting with Vaccine Developers. Viruses, 14.","DOI":"10.3390\/v14112565"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/01652176.2015.1046014","article-title":"Avian Rotavirus Enteritis\u2014An Updated Review","volume":"35","author":"Dhama","year":"2015","journal-title":"Vet. Q."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Louge Uriarte, E.L., Badaracco, A., Spetter, M.J., Mi\u00f1o, S., Armendano, J.I., Zeller, M., Heylen, E., Sp\u00e4th, E., Leunda, M.R., and Moreira, A.R. (2023). Molecular Epidemiology of Rotavirus A in Calves: Evolutionary Analysis of a Bovine G8P[11] Strain and Spatio-Temporal Dynamics of G6 Lineages in the Americas. Viruses, 15.","DOI":"10.3390\/v15102115"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/2\/211\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:25:04Z","timestamp":1760027104000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/2\/211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":55,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["v17020211"],"URL":"https:\/\/doi.org\/10.3390\/v17020211","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,31]]}}}