{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T15:26:34Z","timestamp":1780673194608,"version":"3.54.1"},"reference-count":56,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FBRI SRC VB \u201cVector\u201d Rospotrebnadzor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Highly pathogenic avian influenza (HPAI) H5 clade 2.3.4.4b viruses are widespread in wild and domestic birds, causing severe economic damage to the global poultry industry. Moreover, viruses of this clade are known to cause infections in mammals, posing a potential pandemic threat. Due to the ongoing evolution and change in the dominant strains of H5 clade 2.3.4.4b, it is important to investigate the cross-reactivity of vaccines in use and under development against clade 2.3.4.4b viruses. In this study, the immunogenicity of the previously developed DNA vaccine encoding a modified hemagglutinin of the influenza A\/turkey\/Stavropol\/320-01\/2020 (H5N8) virus, administered by jet injection at doses of 1, 10, 50, 100, and 200 \u03bcg, was investigated. The highest titer of specific to recombinant hemagglutinin antibodies was detected in the group of animals injected with 100 \u00b5g of DNA vaccine. The cross-reactivity study of sera of animals immunized with 100 \u00b5g of DNA vaccine in a microneutralization assay against the strains A\/chicken\/Astrakhan\/321-05\/2020 (H5N8), A\/chicken\/Komi\/24-4V\/2023 (H5N1), and A\/chicken\/Khabarovsk\/24-1V\/2022 (H5N1) showed the formation of cross-neutralizing antibodies. Moreover, the study of protective properties showed that the DNA vaccine protected animals from mortality after infection with A\/chicken\/Khabarovsk\/24-1V\/2022 (H5N1) virus.<\/jats:p>","DOI":"10.3390\/v17030330","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T03:37:57Z","timestamp":1740627477000},"page":"330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dose-Dependent Effect of DNA Vaccine pVAX-H5 Encoding a Modified Hemagglutinin of Influenza A (H5N8) and Its Cross-Reactivity Against A (H5N1) Influenza Viruses of Clade 2.3.4.4b"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2808-4309","authenticated-orcid":false,"given":"Andrey P.","family":"Rudometov","sequence":"first","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria R.","family":"Litvinova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6952-6412","authenticated-orcid":false,"given":"Andrei S.","family":"Gudymo","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia I.","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda B.","family":"Rudometova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis N.","family":"Kisakov","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0768-1561","authenticated-orcid":false,"given":"Mariya B.","family":"Borgoyakova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyubov A.","family":"Kisakova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir A.","family":"Yakovlev","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Tigeeva","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danil I.","family":"Vahitov","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina P.","family":"Makarova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8865-8592","authenticated-orcid":false,"given":"Natalia P.","family":"Kolosova","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2354-7688","authenticated-orcid":false,"given":"Tatiana N.","family":"Ilyicheva","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1339-6732","authenticated-orcid":false,"given":"Vasiliy Yu.","family":"Marchenko","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 630559 Koltsovo, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artemiy A.","family":"Sergeev","sequence":"additional","affiliation":[{"name":"Federal Budgetary Research Institution State Research Center of Virology and Biotechnology Vector, Rospotrebnadzor, 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host factors","volume":"23","author":"Fukuyama","year":"2011","journal-title":"Curr. Opin. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"116534","DOI":"10.1016\/j.socscimed.2023.116534","article-title":"What can we learn from historical pandemics? A systematic review of the literature","volume":"342","author":"Doran","year":"2024","journal-title":"Soc. Sci. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2155072","DOI":"10.1080\/22221751.2022.2155072","article-title":"Alarming situation of emerging H5 and H7 avian influenza and effective control strategies","volume":"12","author":"Shi","year":"2023","journal-title":"Emerg. Microbes Infect."},{"key":"ref_4","first-page":"1","article-title":"From avian to human: Understanding the cross-species transmission and the global spread of highly pathogenic avian influenza","volume":"2","author":"Mohanty","year":"2024","journal-title":"Evidence"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"E774","DOI":"10.1016\/S1473-3099(24)00438-9","article-title":"The panzootic spread of highly pathogenic avian influenza H5N1 sublineage 2.3.4.4 b: A critical appraisal of One Health preparedness and prevention","volume":"24","author":"Koopmans","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105833","DOI":"10.1016\/j.prevetmed.2022.105833","article-title":"Economic impact of highly pathogenic avian influenza outbreaks in Western Java smallholder broiler farms","volume":"212","author":"Pramuwidyatama","year":"2023","journal-title":"Prev. Vet. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.36233\/10.36233\/0507-4088-213","article-title":"Avian flu: \u00abfor whom the bell tolls\u00bb?","volume":"69","author":"Zhirnov","year":"2024","journal-title":"Probl. Virusol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chiba, S., Kiso, M., Yamada, S., Someya, K., Onodera, Y., Yamaguchi, A., Matsunaga, S., Jounai, N., Yamayoshi, S., and Takeshita, F. (2024). Protective effects of an mRNA vaccine candidate encoding H5HA clade 2.3.4.4b against the newly emerged dairy cattle H5N1 virus. eBioMedicine, 109.","DOI":"10.1016\/j.ebiom.2024.105408"},{"key":"ref_9","unstructured":"Centers for Disease Control and Prevention (CDC) (1997). Isolation of avian influenza A(H5N1) viruses from humans\u2014Hong Kong, May\u2013December 1997. MMWR Morb. Mortal. Wkly. Rep., 46, 1204\u20131207."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1126\/science.1062882","article-title":"Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses","volume":"293","author":"Hatta","year":"2001","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Focosi, D., and Maggi, F. (2024). Avian Influenza Virus A(H5Nx) and Prepandemic Candidate Vaccines: State of the Art. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25158550"},{"key":"ref_12","unstructured":"World Health Organization (2024, December 04). Cumulative Number of Confirmed Human Cases for Avian Influenza A(H5N1) Reported to WHO, 2003\u20132024, 27 September 2024. Available online: https:\/\/www.who.int\/publications\/m\/item\/cumulative-number-of-confirmed-human-cases-for-avian-influenza-a(h5n1)-reported-to-who--2003-2024--27-september-2024."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/s41586-023-06631-2","article-title":"The episodic resurgence of highly pathogenic avian influenza H5 virus","volume":"622","author":"Xie","year":"2023","journal-title":"Nature"},{"key":"ref_14","unstructured":"World Health Organization (2024, November 17). Assessment of Risk Associated with Recent Influenza A(H5N1) Clade 2.3.4.4b Viruses 21 December 2022. Available online: https:\/\/cdn.who.int\/media\/docs\/default-source\/influenza\/avian-and-other-zoonotic-influenza\/h5-risk-assessment-dec-2022.pdf?sfvrsn\u2009=\u2009a496333a_1&download\u2009=\u2009true."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2100439","DOI":"10.2807\/1560-7917.ES.2021.26.24.2100439","article-title":"Isolation of clade 2.3.4.4b A(H5N8), a highly pathogenic avian influenza virus, from a worker during an outbreak on a poultry farm, Russia, December 2020","volume":"26","author":"Pyankova","year":"2021","journal-title":"Euro Surveill."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/s41586-024-07766-6","article-title":"Pathogenicity and transmissibility of bovine H5N1 influenza virus","volume":"633","author":"Eisfeld","year":"2024","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.3201\/eid3007.240508","article-title":"Highly pathogenic avian influenza A (H5N1) clade 2.3.4.4 b virus infection in domestic dairy cattle and cats, United States, 2024","volume":"30","author":"Burrough","year":"2024","journal-title":"Emerg. Infect Dis."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Le Sage, V., Werner, B.D., Merrbach, G.A., Petnuch, S.E., O\u2019Connell, A.K., Simmons, H.C., McCarthy, K.R., Reed, D.S., Moncla, L.H., and Bhavsar, D. (2024). Pre-existing H1N1 immunity reduces severe disease with bovine H5N1 influenza virus. bioRxiv.","DOI":"10.1101\/2024.10.23.619881"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"taad032","DOI":"10.1093\/jtm\/taad032","article-title":"First case of human infection with highly pathogenic H5 avian influenza a virus in South America: A new zoonotic pandemic threat for 2023?","volume":"30","author":"Bruno","year":"2023","journal-title":"J. Travel Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2332667","DOI":"10.1080\/22221751.2024.2332667","article-title":"Highly pathogenic avian influenza A (H5N1) virus of clade 2.3.4.4 b isolated from a human case in Chile causes fatal disease and transmits between co-housed ferrets","volume":"13","author":"Brock","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s44298-024-00039-z","article-title":"Panzootic HPAIV H5 and risks to novel mammalian hosts","volume":"2","author":"Abdelwhab","year":"2024","journal-title":"npj Viruses"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.virol.2014.03.008","article-title":"Enhanced human receptor binding by H5 haemagglutinins","volume":"456\u2013457","author":"Xiong","year":"2014","journal-title":"Virology"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Kok, A., Fouchier, R.A.M., and Richard, M. (2021). Cross-Reactivity Conferred by Homologous and Heterologous Prime-Boost A\/H5 Influenza Vaccination Strategies in Humans: A Literature Review. Vaccines, 9.","DOI":"10.3390\/vaccines9121465"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1126\/science.adg8342","article-title":"Bird flu spread between mink is a \u2018warning bell\u2019","volume":"379","author":"Kupferschmidt","year":"2023","journal-title":"Science"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/d41586-023-00201-2","article-title":"Bird flu outbreak in mink sparks concern about spread in people","volume":"614","author":"Sidik","year":"2023","journal-title":"Nature"},{"key":"ref_26","first-page":"2300001","article-title":"Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, October 2022","volume":"28","author":"Monne","year":"2023","journal-title":"Euro Surveill."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.3201\/eid2711.211281","article-title":"Highly Pathogenic Avian Influenza A(H5N1) Virus in Wild Red Foxes, the Netherlands, 2021","volume":"27","author":"Rijks","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"786","DOI":"10.3201\/eid2904.221538","article-title":"Highly Pathogenic Avian Influenza A(H5N1) Virus Outbreak in New England Seals, United States","volume":"29","author":"Puryear","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Marchenko, V.Y., Panova, A.S., Kolosova, N.P., Gudymo, A.S., Svyatchenko, S.V., Danilenko, A.V., Vasiltsova, N.N., Egorova, M.L., Onkhonova, G.S., and Zhestkov, P.D. (2024). Characterization of H5N1 avian influenza virus isolated from bird in Russia with the E627K mutation in the PB2 protein. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-78175-y"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1038\/s41591-024-03189-y","article-title":"Licensed H5N1 vaccines generate cross-neutralizing antibodies against highly pathogenic H5N1 clade 2.3.4.4b influenza virus","volume":"30","author":"Khurana","year":"2024","journal-title":"Nat. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Litvinova, V.R., Rudometov, A.P., Rudometova, N.B., Kisakov, D.N., Borgoyakova, M.B., Kisakova, L.A., Starostina, E.V., Fando, A.A., Yakovlev, V.A., and Tigeeva, E.V. (2024). DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge. Vaccines, 12.","DOI":"10.3390\/vaccines12050538"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Rudometova, N.B., Fando, A.A., Kisakova, L.A., Kisakov, D.N., Borgoyakova, M.B., Litvinova, V.R., Yakovlev, V.A., Tigeeva, E.V., Vahitov, D.I., and Sharabrin, S.V. (2024). Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus. Vaccines, 12.","DOI":"10.3390\/vaccines12020143"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/s41421-023-00571-x","article-title":"Potential cross-species transmission of highly pathogenic avian influenza H5 subtype (HPAI H5) viruses to humans calls for the development of H5-specific and universal influenza vaccines","volume":"9","author":"Huang","year":"2023","journal-title":"Cell Discov."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sultankulova, K.T., Argimbayeva, T.U., Aubakir, N.A., Bopi, A., Omarova, Z.D., Melisbek, A.M., Karamendin, K., Kydyrmanov, A., Chervyakova, O.V., and Kerimbayev, A.A. (2024). Reassortants of the Highly Pathogenic Influenza Virus A\/H5N1 Causing Mass Swan Mortality in Kazakhstan from 2023 to 2024. Animals, 14.","DOI":"10.3390\/ani14223211"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Gudymo, A., Onkhonova, G., Danilenko, A., Susloparov, I., Danilchenko, N., Kosenko, M., Moiseeva, A., Kolosova, N., Svyatchenko, S., and Marchenko, V. (2023). Quantitative Assessment of Airborne Transmission of Human and Animal Influenza Viruses in the Ferret Model. Atmosphere, 14.","DOI":"10.3390\/atmos14030471"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Saad, N., Esaki, M., Kojima, I., Khalil, A.M., Osuga, S., Shahein, M.A., Okuya, K., Ozawa, M., and Alhatlani, B.Y. (2024). Phylogenetic Characterization of Novel Reassortant 2.3.4.4b H5N8 Highly Pathogenic Avian Influenza Viruses Isolated from Domestic Ducks in Egypt During the Winter Season 2021\u20132022. Viruses, 16.","DOI":"10.3390\/v16111655"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1134\/S1068162023020152","article-title":"mRNA vaccine platform: mRNA production and delivery","volume":"49","author":"Litvinova","year":"2023","journal-title":"Russ. J. Bioorg. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lu, B., Lim, J.M., Yu, B., Song, S., Neeli, P., Sobhani, N., Pavithra, K., Bonam, S.R., Kurapati, R., and Zheng, J. (2024). The next-generation DNA vaccine platforms and delivery systems: Advances, challenges and prospects. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1332939"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e2115379119","DOI":"10.1073\/pnas.2115379119","article-title":"Universal stabilization of the influenza hemagglutinin by structure-based redesign of the pH switch regions","volume":"119","author":"Milder","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","first-page":"pgae462","article-title":"Engineering a cleaved, prefusion-stabilized influenza B virus hemagglutinin by identification and locking of all six pH switches","volume":"3","author":"Juraszek","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA Nexus"},{"key":"ref_41","unstructured":"World Health Organization (2024, November 17). Recommendations for Influenza Vaccine Composition. Available online: https:\/\/www.who.int\/teams\/global-influenza-programme\/vaccines\/who-recommendations."},{"key":"ref_42","first-page":"48","article-title":"Review on the Epizootiological Situation on Highly Pathogenic Avian Influenza around the World and in Russia in 2022","volume":"1","author":"Marchenko","year":"2023","journal-title":"Probl. Part. Danger. Infect."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Kozak, M., and Hu, J. (2024). DNA Vaccines: Their Formulations, Engineering and Delivery. Vaccines, 12.","DOI":"10.3390\/vaccines12010071"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1016\/j.ymthe.2023.03.012","article-title":"Neuraminidase delivered as an APC-targeted DNA vaccine induces protective antibodies against influenza","volume":"31","author":"Werninghaus","year":"2023","journal-title":"Mol. Ther."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zhang, J., Li, C., Liu, Y., Liao, R., He, D., Xu, L., Chen, T., Xiao, Q., Luo, M., and Chen, Y. (2024). Airway applied IVT mRNA vaccine needs specific sequence design and high standard purification that removes devastating dsRNA contaminant. bioRxiv.","DOI":"10.1101\/2024.09.22.614306"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s10517-024-06102-2","article-title":"Removal of Double-Stranded RNA Contaminants During Template-Directed Synthesis of mRNA","volume":"176","author":"Sharabrin","year":"2024","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e142","DOI":"10.1093\/nar\/gkr695","article-title":"Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA","volume":"39","author":"Muramatsu","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nsmb.2934","article-title":"Human cells contain natural double-stranded RNAs with potential regulatory functions","volume":"22","author":"Portal","year":"2015","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_49","unstructured":"World Health Organization (2025, February 18). Genetic and Antigenic Characteristics of Clade 2.3.4.4b A(H5N1) Viruses Identified in Dairy Cattle in the United States of America. Available online: https:\/\/dev-cms.who.int\/publications\/m\/item\/genetic-and-antigenic-characteristics-of-clade-2.3.4.4b-a(h5n1)-viruses-identified-in-dairy-cattle-in-the-united-states-of-america."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"707","DOI":"10.3390\/vaccines2040707","article-title":"Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future","volume":"2","author":"Trombetta","year":"2014","journal-title":"Vaccines"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1073\/pnas.0813390106","article-title":"Adjuvanted H5N1 vaccine induces early CD4+ T cell response that predicts long-term persistence of protective antibody levels","volume":"106","author":"Galli","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1016\/j.vaccine.2012.07.077","article-title":"Cell culture (Vero cell) derived whole-virus non-adjuvanted H5N1 influenza vaccine induces long-lasting cross-reactive memory immune response: Homologous or heterologous booster response following two dose or single dose priming","volume":"30","author":"Aichinger","year":"2012","journal-title":"Vaccine"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1038\/s41467-024-48555-z","article-title":"Development of a nucleoside-modified mRNA vaccine against clade 2.3.4.4b H5 highly pathogenic avian influenza virus","volume":"15","author":"Furey","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1038\/s41541-024-00988-9","article-title":"An mRNA vaccine candidate encoding H5HA clade 2.3.4.4b protects mice from clade 2.3.2.1a virus infection","volume":"9","author":"Chiba","year":"2024","journal-title":"npj Vaccines"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1038\/s41541-024-00932-x","article-title":"Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza","volume":"9","author":"Cui","year":"2024","journal-title":"npj Vaccines"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1002\/mlf2.12085","article-title":"Protective efficacy of a universal influenza mRNA vaccine against the challenge of H1 and H5 influenza A viruses in mice","volume":"2","author":"Li","year":"2023","journal-title":"mLife"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/3\/330\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:43:06Z","timestamp":1760028186000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/3\/330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":56,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["v17030330"],"URL":"https:\/\/doi.org\/10.3390\/v17030330","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]}}}