{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:29:08Z","timestamp":1781886548683,"version":"3.54.5"},"reference-count":41,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Show Chwan Memorial Hospital, Taiwan","award":["R11028"],"award-info":[{"award-number":["R11028"]}]},{"name":"Show Chwan Memorial Hospital, Taiwan","award":["KMUH110-0R34"],"award-info":[{"award-number":["KMUH110-0R34"]}]},{"name":"Kaohsiung Medical University Hospital, Taiwan","award":["R11028"],"award-info":[{"award-number":["R11028"]}]},{"name":"Kaohsiung Medical University Hospital, Taiwan","award":["KMUH110-0R34"],"award-info":[{"award-number":["KMUH110-0R34"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>(1) Background: The C-type lectin domain family 4 member M (CLEC4M, also known as L-SIGN) is a crucial pathogen-recognition receptor for the dengue virus (DENV). Our previous study has exhibited a polymorphism in its extracellular neck region, specifically within the long tandem repeats of exon 4, which correlates with DHF in DENV infection and causes liver damage. (2) Methods: Using monocyte-derived dendritic cells (MDDCs) and SK-HEP1 liver endothelial cell lines to compare viral replication relative to L-SIGN expression. (3) Results: Results indicated that SK-HEP1 cells were more susceptible to DENV infection than MDDCs, and L-SIGN transfection significantly increased viral replication in SK-HEP1 cell lines. The study also found that L-SIGN-enhanced DENV infection is mediated by the decrease in monocyte chemoattractant protein-1 (MCP-1) but not interferon gamma inducible protein-10 (IP-10). These findings reveal that L-SIGN-induced DENV infection leads to reduced MCP-1 levels, which, in turn, enhances DENV replication velocity. (4) Conclusions: This study offers insights into the molecular mechanisms of DENV replication and identifies potential therapeutic targets involving MCP-1 and L-SIGN pathways.<\/jats:p>","DOI":"10.3390\/v17030344","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T04:28:18Z","timestamp":1740716898000},"page":"344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["MCP-1 Reduction by L-SIGN Expression in Dengue Virus-Infected Liver Endothelial Cells"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9504-0413","authenticated-orcid":false,"given":"Keh-Sen","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Show Chwan Memorial Hospital, Changhua 500, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Pojen Hospital, Kaohsiung 807, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0824-9615","authenticated-orcid":false,"given":"Po-Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Research Assistant Center, Show Chwan Memorial Hospital, Changhua 500, Taiwan"},{"name":"Department of Nursing, Central Taiwan University of Science and Technology, Taichung 406, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ing-Kit","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung Medical Center, Kaohsiung 803, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuender D.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Research, MacKay Memorial Hospital, Taipei 104, Taiwan"},{"name":"Department of Pediatrics, MacKay Memorial Hospital, Taipei 104, Taiwan"},{"name":"Department of Medicine, MacKay Medical College, New Taipei 252, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7529-5390","authenticated-orcid":false,"given":"Rong-Fu","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan"},{"name":"Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"ref_1","first-page":"e10678","article-title":"Unusual Manifestations of Dengue Fever: A Review on Expanded Dengue Syndrome","volume":"12","author":"Umakanth","year":"2020","journal-title":"Cureus"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jaenisch, T., Tam, D.T., Kieu, N.T., Van Ngoc, T., Nam, N.T., Van Kinh, N., Yacoub, S., Chanpheaktra, N., Kumar, V., and See, L.L.C. 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