{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:56:19Z","timestamp":1791654979970,"version":"4.3.4"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Key Technologies R&amp;D Program of the National Ministry of Science","award":["2018ZX10713002"],"award-info":[{"award-number":["2018ZX10713002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>To investigate the distribution of hantavirus (HV) in rodent organs, we selected eight counties across four regions in Hebei Province (southern, northern, eastern, and central) as study areas. Rodents were captured using night trapping methods, and organ samples were aseptically collected for HV detection via quantitative real-time PCR (qPCR) and gene sequencing. During the 2022\u20132023 spring and autumn seasons, 1386 rodents were trapped, including 73 Rattus norvegicus carrying Seoul virus (SEOV). The highest detection rate was observed in the liver (3.84%), followed by the kidneys (3.46%) and lungs (3.09%). Viral load analysis revealed higher SEOV RNA levels in the liver than in the lungs and kidneys. Antibody levels in R. norvegicus may influence the detection of viruses in organs. Phylogenetic analysis indicated that all sequences belonged to the S3 subtype, exhibiting regional aggregation and genetic stability. Our findings emphasize the necessity of multi-organ sampling for comprehensive HV surveillance and epidemic risk assessment.<\/jats:p>","DOI":"10.3390\/v17030412","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T04:29:18Z","timestamp":1741926558000},"page":"412","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Distribution and Genetic Characteristics of Seoul Virus in Different Organs of Rattus norvegicus"],"prefix":"10.3390","volume":"17","author":[{"given":"Yamei","family":"Wei","sequence":"first","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanying","family":"Han","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggang","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Han","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Hebei Provincial Centre for Disease Control and Prevention, Shijiazhuang 050021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1093\/infdis\/137.3.298","article-title":"Isolation of the etiologic agent of Korean Hemorrhagic fever","volume":"137","author":"Lee","year":"1978","journal-title":"J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e106924","DOI":"10.1128\/jvi.01069-24","article-title":"Promotion of order Bunyavirales to class Bunyaviricetes to accommodate a rapidlyincreasing number of related polyploviricotine viruses","volume":"98","author":"Kuhn","year":"2024","journal-title":"J. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1111\/1469-0691.12291","article-title":"Hantavirus infections","volume":"21","author":"Saksida","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.1099\/0022-1317-77-11-2677","article-title":"Hantaviruses: Genome structure, expression and evolution","volume":"77","author":"Plyusnin","year":"1996","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Jiang, H., Du, H., Wang, L.M., Wang, P.Z., and Bai, X.F. (2016). Hemorrhagic Fever with Renal Syndrome: Pathogenesis and Clinical Picture. Front. Cell Infect. Microbiol., 6.","DOI":"10.3389\/fcimb.2016.00001"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, N., Li, A., Liu, Y., Wu, W., Li, C., Yu, D., Zhu, Y., Li, J., Li, D., and Wang, S. (2020). Genetic diversity and evolution of Hantaan virus in China and its neighbors. PLoS Neglect. Trop. D., 14.","DOI":"10.1101\/2020.01.28.922724"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1080\/09603123.2021.1917527","article-title":"Epidemiology, virology and clinical aspects of hantavirus infections: An overview","volume":"32","author":"Singh","year":"2022","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Muyangwa, M., Martynova, E.V., Khaiboullina, S.F., Morzunov, S.P., and Rizvanov, A.A. (2015). Hantaviral Proteins: Structure, Functions, and Role in Hantavirus Infection. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.01326"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Afzal, S., Ali, L., Batool, A., Afzal, M., Kanwal, N., Hassan, M., Safdar, M., Ahmad, A., and Yang, J. (2023). Hantavirus: An overview and advancements in therapeutic approaches for infection. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1233433"},{"key":"ref_10","first-page":"w13937","article-title":"Human hantavirus infections: Epidemiology, clinical features, pathogenesis and immunology","volume":"144","author":"Manigold","year":"2014","journal-title":"Swiss Med. Wkly."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kuhn, J.H., and Schmaljohn, C.S. (2023). A Brief History of Bunyaviral Family Hantaviridae. Diseases, 11.","DOI":"10.3390\/diseases11010038"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.3201\/eid1608.090470","article-title":"Hantavirus infections in humans and animals, China","volume":"16","author":"Zhang","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3201\/eid3001.230855","article-title":"Hantavirus Disease Cluster Caused by Seoul Virus, Germany","volume":"30","author":"Hofmann","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"200","DOI":"10.3201\/eid1502.080291","article-title":"Seoul virus and hantavirus disease, Shenyang, People\u2019s Republic of China","volume":"15","author":"Zhang","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Alburkat, H., Smura, T., Bouilloud, M., Pradel, J., Anfray, G., Berthier, K., Dutra, L., Loiseau, A., Niamsap, T., and Olander, V. (2024). Evolution and genetic characterization of Seoul virus in wild rats Rattus norvegicus from an urban park in Lyon, France 2020\u20132022. PLoS Negl. Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0012142"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wang, Q., Yue, M., Yao, P., Zhu, C., Ai, L., Hu, D., Zhang, B., Yang, Z., Yang, X., and Luo, F. (2021). Epidemic Trend and Molecular Evolution of HV Family in the Main Hantavirus Epidemic Areas From 2004 to 2016, in P.R. China. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.584814"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.3390\/v6031317","article-title":"Hantavirus immunology of rodent reservoirs: Current status and future directions","volume":"6","author":"Schountz","year":"2014","journal-title":"Viruses"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0966-842X(99)01658-3","article-title":"Persistent hantavirus infections: Characteristics and mechanisms","volume":"8","author":"Meyer","year":"2000","journal-title":"Trends Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Madai, M., Horvath, G., Herczeg, R., Somogyi, B., Zana, B., Foldes, F., Kemenesi, G., Kurucz, K., Papp, H., and Zeghbib, S. (2021). Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine. Viruses, 13.","DOI":"10.20944\/preprints202101.0192.v1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/rmv.1722","article-title":"Hantavirus infections in Europe and their impact on public health","volume":"23","author":"Vaheri","year":"2013","journal-title":"Rev. Med. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1586\/14787210.7.2.205","article-title":"Hantavirus infections in Europe: From virus carriers to a major public-health problem","volume":"7","author":"Heyman","year":"2009","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3947\/ic.2021.0148","article-title":"Hemorrhagic Fever with Renal Syndrome: Literature Review, Epidemiology, Clinical Picture and Pathogenesis","volume":"54","author":"Tariq","year":"2022","journal-title":"Infect. Chemother."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Honig, V., Kamis, J., Marsikova, A., Matejkova, T., Stopka, P., Macova, A., Ruzek, D., and Kvicerova, J. (2022). Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.01306-22"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1128\/JVI.77.2.1540-1550.2002","article-title":"Persistent Sin Nombre virus infection in the deer mouse (Peromyscus maniculatus) model: Sites of replication and strand-specific expression","volume":"77","author":"Botten","year":"2003","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.bbi.2007.10.005","article-title":"Sex differences in the recognition of and innate antiviral responses to Seoul virus in Norway rats","volume":"22","author":"Hannah","year":"2008","journal-title":"Brain Behav. Immun."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"He, W., Fu, J., Wen, Y., Cheng, M., Mo, Y., and Chen, Q. (2021). Detection and Genetic Characterization of Seoul Virus in Liver Tissue Samples From Rattus norvegicus and Rattus tanezumi in Urban Areas of Southern China. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.748232"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1089\/vbz.2017.2204","article-title":"Parallel Survey of Two Widespread Renal Syndrome-Causing Zoonoses: Leptospira spp. and Hantavirus in Urban Environment, Hungary","volume":"18","author":"Kurucz","year":"2018","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nrmicro3066","article-title":"Uncovering the mysteries of hantavirus infections","volume":"8","author":"Vaheri","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1016\/j.micinf.2006.04.019","article-title":"Hantavirus cardiopulmonary syndrome: Immune response and pathogenesis","volume":"8","author":"Borges","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1128\/JVI.73.1.585-591.1999","article-title":"Sin Nombre virus pathogenesis in Peromyscus maniculatus","volume":"73","author":"Netski","year":"1999","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1099\/vir.0.000076","article-title":"Life-long shedding of Puumala hantavirus in wild bank voles (Myodes glareolus)","volume":"96","author":"Voutilainen","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1017\/S0950268814001009","article-title":"Hantavirus infection in rodents and haemorrhagic fever with renal syndrome in Shaanxi province, China, 1984\u20132012","volume":"143","author":"Yu","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Pang, Z., Li, A., Li, J., Qu, J., He, C., Zhang, S., Li, C., Zhang, Q., Liang, M., and Li, D. (2014). Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0095635"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1128\/JVI.00725-11","article-title":"Migration of Norway rats resulted in the worldwide distribution of Seoul hantavirus today","volume":"86","author":"Lin","year":"2012","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3201\/eid2901.221092","article-title":"Role of Seaports and Imported Rats in Seoul Hantavirus Circulation, Africa","volume":"29","author":"Castel","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e1914","DOI":"10.1128\/mBio.01933-14","article-title":"Detection of zoonotic pathogens and characterization of novel viruses carried by commensal Rattus norvegicus in New York City","volume":"5","author":"Firth","year":"2014","journal-title":"mBio"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1007\/s12250-017-3973-z","article-title":"Detection and characterization of three zoonotic viruses in wild rodents and shrews from Shenzhen city, China","volume":"32","author":"Wang","year":"2017","journal-title":"Virol. Sin."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"249","DOI":"10.3201\/eid2402.171216","article-title":"Multiplex PCR-Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats","volume":"24","author":"Kim","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_39","first-page":"136","article-title":"Detection of Hantavirus RNA in Different Organs of Natural Infected Rattus norvegicus","volume":"21","author":"Jiang","year":"2006","journal-title":"Virol. Sin."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.micinf.2008.12.016","article-title":"The hantaviral load in tissues of naturally infected rodents","volume":"11","author":"Korva","year":"2009","journal-title":"Microbes Infect."},{"key":"ref_41","first-page":"217","article-title":"Emergence and persistence of hantaviruses","volume":"315","author":"Klein","year":"2007","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Easterbrook, J.D., and Klein, S.L. (2008). Immunological mechanisms mediating hantavirus persistence in rodent reservoirs. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000172"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1007\/s00705-011-1013-0","article-title":"Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction","volume":"156","author":"Nemeth","year":"2011","journal-title":"Arch. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Cao, S., Ma, J., Cheng, C., Ju, W., and Wang, Y. (2016). Genetic characterization of hantaviruses isolated from rodents in the port cities of Heilongjiang, China, in 2014. BMC Vet. Res., 12.","DOI":"10.1186\/s12917-016-0695-7"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1743-422X-11-32","article-title":"Detection and genetic characterization of Seoul virus from commensal brown rats in France","volume":"11","author":"Dupinay","year":"2014","journal-title":"Virol. J."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/3\/412\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:53:27Z","timestamp":1760028807000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/3\/412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,14]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["v17030412"],"URL":"https:\/\/doi.org\/10.3390\/v17030412","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,14]]}}}