{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:35:57Z","timestamp":1784590557331,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Research and Scientific and Technological Development Support of Maranh\u00e3o\/Research Program for the SUS (PPSUS): Shared Management in Health-PPSUS 2020 Program (PPSUS-FAPEMA\/MS-DECIT\/CNPq\/SESMA)","award":["N\u00ba02052\/20"],"award-info":[{"award-number":["N\u00ba02052\/20"]}]},{"name":"National Council for Scientific and Technological Development (CNPq)","award":["N\u00ba02052\/20"],"award-info":[{"award-number":["N\u00ba02052\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Arboviruses transmitted by Aedes aegypti cause high number of cases and deaths annually. The aim was to investigate the presence of the presence of Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIKV) viruses in endemic areas of Maranh\u00e3o, northeastern Brazil. The study was carried out in Caxias, Cod\u00f3, Peritor\u00f3, and S\u00e3o Mateus do Maranh\u00e3o in 2019 (Caxias) and 2022. The blood samples were subjected to RNA extraction and then tested by RT-qPCR. Cell culture was used to attempt viral isolation and subsequent sequencing. In total, 171 samples were analyzed (32 from 2019, 18.7%) and 72 (42.1%) were found to have arboviruses: 68 (39.7%) from Caxias; 2 (1.1%) from Cod\u00f3; 1 (0.6%) from Peritor\u00f3; and 1 (0.6%) from S\u00e3o Mateus. Overall, 85.3% (n = 58) of the positive samples were infected with DENV-1, 4 (four) (5.9%) with DENV-2 (Caxias), 1 (one) (1.5%) with DENV-3 (Caxias), and in 6 (six) (7.3%) samples CHIKV was detected, with one co-infection of DENV-1 and CHIKV (Caxias). The DENV-1 genotype V and the ECSA genotype of CHIKV were characterized in samples from Caxias. The detection of DENV-1, DENV-2, DENV-3, and more CHIKV in the interior of Maranh\u00e3o alerts to the importance of virological studies in these areas.<\/jats:p>","DOI":"10.3390\/v17040475","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T06:06:35Z","timestamp":1743141995000},"page":"475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Retrospective Study of Arbovirus Circulation in Northeast Brazil in 2019 and 2022: Insights into the Re-Emergence of DENV-3 and the Co-Infection of DENV-1 and CHIKV"],"prefix":"10.3390","volume":"17","author":[{"given":"S\u00eamilly Su\u00e9len da Silva","family":"Sousa","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Rede BIONORTE, Universidade Estadual do Maranh\u00e3o\u2014UEMA, S\u00e3o Luis 65055-310, Brazil"},{"name":"Laborat\u00f3rio de Entomologia M\u00e9dica\u2014LABEM, Universidade Estadual do Maranh\u00e3o\u2014UEMA, Campus Caxias, Caxias 65604-380, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"},{"name":"Centro de Ciencias Biol\u00f3gicas e da Sa\u00fade, Universidade Estadual do Par\u00e1\u2014UEPA, Bel\u00e9m 66087-662, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-6496","authenticated-orcid":false,"given":"Carine Fortes","family":"Arag\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glennda Juscely Galv\u00e3o Pereira","family":"Cereja","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7185-4538","authenticated-orcid":false,"given":"Sandro Patroca da","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raira Maria Morais de","family":"Sousa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia M\u00e9dica\u2014LABEM, Universidade Estadual do Maranh\u00e3o\u2014UEMA, Campus Caxias, Caxias 65604-380, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9769-2183","authenticated-orcid":false,"given":"Murilo Tavares","family":"Amorim","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3319-1854","authenticated-orcid":false,"given":"Eliana Vieira Pinto","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Tardelli Diniz","family":"Nunes","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4652-4884","authenticated-orcid":false,"given":"Val\u00e9ria Cristina Soares","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Rede BIONORTE, Universidade Estadual do Maranh\u00e3o\u2014UEMA, S\u00e3o Luis 65055-310, Brazil"},{"name":"Laborat\u00f3rio de Entomologia M\u00e9dica\u2014LABEM, Universidade Estadual do Maranh\u00e3o\u2014UEMA, Campus Caxias, Caxias 65604-380, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","unstructured":"World Health Organisation (WHO) (2025, February 21). 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