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This study investigated the prevalence, genetic diversity, and molecular characteristics of HAdV-F40\/41 in AGE cases reported in Brazil from 2021 to 2023, a period after the COVID-19 pandemic. A total of 1980 stool samples collected from medically attended AGE patients from nine states were analyzed by TaqMan-based qPCR. Overall, HAdV was detected in 16.6% (n = 328\/1980) of cases, with the highest prevalence observed in children under five years of age. The positive HAdV samples were genotyped through partial sequencing of the hexon and\/or fiber genes followed by phylogenetic analysis. Enteric HAdVs (HAdV-F40\/41) were detected in 3.2% (n = 63\/1980) of samples, with HAdV-F41 (44.1%) being the most common genotype. Among the non-enteric types, HAdV-C (29.4%) was the most prevalent, followed by HAdV-B (13.2%), HAdV-A (10.3%), and HAdV-D (2.9%). Phylogenetic analysis of the hexon (HVR1\u2013HVR6) and fiber (Shaft) gene regions identified two major clusters, H-GTC1 and F-GTC2, showing close genetic relationships with global strains. HAdV-F40\/41 demonstrated significantly higher viral loads compared to non-enteric HAdVs. These findings highlight the importance of continued surveillance of HAdV-F to better understand its role in AGE cases and support public health strategies, including potential vaccine development.<\/jats:p>","DOI":"10.3390\/v17040577","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T06:53:46Z","timestamp":1744872826000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular Epidemiology of Human Adenovirus from Acute Gastroenteritis Cases in Brazil After the COVID-19 Pandemic Period, 2021\u20132023"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-7787","authenticated-orcid":false,"given":"Mateus de Souza","family":"Mello","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21045-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Correia","family":"Malta","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21045-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Madi","family":"Fialho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21045-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9933-7161","authenticated-orcid":false,"given":"Fernanda Marcicano","family":"Burlandy","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21045-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9654-0172","authenticated-orcid":false,"given":"Tulio Machado","family":"Fumian","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz (IOC), Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21045-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","article-title":"Burden and Aetiology of Diarrhoeal Disease in Infants and Young Children in Developing Countries (the Global Enteric Multicenter Study, GEMS): A Prospective, Case-Control Study","volume":"382","author":"Kotloff","year":"2013","journal-title":"Lancet"},{"key":"ref_2","unstructured":"(2023, December 30). 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