{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:55:18Z","timestamp":1791352518708,"version":"4.3.1"},"reference-count":91,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda, e Innovaci\u00f3n de Mexico (Secretariat of Science, Humanities, Technology, and Innovation of Mexico)"},{"name":"Instituto de Ciencia, Tecnolog\u00eda, e Inovaci\u00f3n del Estado de Chiapas (Institute of Science, Technology, and Innovation of the State of Chiapas)"},{"name":"Natura y Ecosistemas Mexicanos A.C. (Mexican Nature and Ecosystems Civil Association)"},{"name":"College of Veterinary Medicine at Iowa State University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>We performed serologic surveillance for selected arthropod-borne viruses (arboviruses) in bats and opossums in the Lacandona Rainforest, Chiapas, Mexico, in 2023\u20132024. Sera were collected from 94 bats of at least 15 species and 43 opossums of three species. The sera were assayed by the plaque reduction neutralization test (PRNT) for antibodies to eight orthoflaviviruses (dengue viruses 1\u20134, St. Louis encephalitis virus, T\u2019Ho virus, West Nile virus, and Zika virus) and one alphavirus (chikungunya virus; CHIKV). Twelve (12.8%) bats and 15 (34.9%) opossums contained orthoflavivirus-specific antibodies. One bat (a Jamaican fruit bat) was seropositive for Zika virus, and 11 bats contained antibodies to an undetermined orthoflavivirus, as did the 15 opossums. All bats and most opossums seropositive for an undetermined orthoflavivirus had low PRNT titers, possibly because they had been infected with another (perhaps unrecognized) orthoflavivirus not included in the PRNTs. Antibodies that neutralized CHIKV were detected in three (7.0%) opossums and none of the bats. The three opossums had low CHIKV PRNT titers, and therefore, another alphavirus may have been responsible for the infections. In summary, we report serologic evidence of arbovirus infections in bats and opossums in Chiapas, Mexico.<\/jats:p>","DOI":"10.3390\/v17050590","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T20:24:52Z","timestamp":1745353492000},"page":"590","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Serologic Surveillance for Orthoflaviviruses and Chikungunya Virus in Bats and Opossums in Chiapas, Mexico"],"prefix":"10.3390","volume":"17","author":[{"given":"J. Manuel","family":"Aranda-Coello","sequence":"first","affiliation":[{"name":"Departamento de Conservaci\u00f3n de la Biodiversidad, El Colegio de la Frontera Sur, Lerma 24500, Campeche, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Machain-Williams","sequence":"additional","affiliation":[{"name":"Estudios en Una Salud, Unidad Profesional Interdisciplinaria de Ingenier\u00eda Campus Palenque, Instituto Polit\u00e9cnico Nacional, Palenque 29960, Chiapas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0060-7729","authenticated-orcid":false,"given":"Manuel","family":"Weber","sequence":"additional","affiliation":[{"name":"Departamento de Conservaci\u00f3n de la Biodiversidad, El Colegio de la Frontera Sur, Lerma 24500, Campeche, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma R.","family":"Dzul Rosado","sequence":"additional","affiliation":[{"name":"Estudios en Una Salud, Unidad Profesional Interdisciplinaria de Ingenier\u00eda Campus Palenque, Instituto Polit\u00e9cnico Nacional, Palenque 29960, Chiapas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler R.","family":"Simpkins","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9857-5755","authenticated-orcid":false,"given":"Bradley J.","family":"Blitvich","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Campos, R.K., Rossi, S.L., Tesh, R.B., and Weaver, S.C. 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