{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T13:24:58Z","timestamp":1784813098617,"version":"3.55.0"},"reference-count":22,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Florida, Institute for Food and Agricultural Sciences, Cervidae Health Research In-itiative, funded through the Florida State Legislature","award":["LBR2199"],"award-info":[{"award-number":["LBR2199"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>A novel ephemeral fever rhabdovirus and a CHeRI orbivirus of a previously unidentified genetic lineage were isolated in mosquito cell line C6\/36 cells as co-infecting agents from the spleen tissue of a dead farmed white-tailed deer (WTD; Odocoileus virginianus) in Florida. We designated the ephemeral fever rhabdovirus as Hardee County ephemerovirus 1, strain CHeRI ephemerovirus 1. The genetic sequences of the CHeRI orbivirus isolated in this work differ significantly from those of three previously described CHeRI orbivirus lineages. We designated this new virus as CHeRI orbivirus 4, strain CHeRI orbivirus 4-1. Whereas it remains unknown whether one, both, or none of the viruses contributed to the pathology, gross observations revealed that the dead WTD had severely congested and hemorrhagic lungs, and that its heart, kidneys, and spleen were also congested.<\/jats:p>","DOI":"10.3390\/v17050614","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:08:24Z","timestamp":1745554104000},"page":"614","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (Odocoileus virginianus) in Florida, USA"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8780-3745","authenticated-orcid":false,"given":"Emily","family":"DeRuyter","sequence":"first","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA"},{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8171-4053","authenticated-orcid":false,"given":"Pedro H. O.","family":"Viadanna","sequence":"additional","affiliation":[{"name":"Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Wilson","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"},{"name":"Department of Wildlife Ecology and Conservation, College Agricultural and Life Sciences, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"White","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"},{"name":"Department of Wildlife Ecology and Conservation, College Agricultural and Life Sciences, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6384-0589","authenticated-orcid":false,"given":"Merrie","family":"Urban","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7368-4234","authenticated-orcid":false,"given":"Pacharapong","family":"Khrongsee","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"},{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Faculty of Veterinary Science, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6992-1295","authenticated-orcid":false,"given":"Thais C. S.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Operation GRACE, National Marine Mammal Foundation, San Diego, CA 92106, USA"},{"name":"Associa\u00e7\u00e3o R3 Animal, Florian\u00f3polis 88061-500, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas B.","family":"Waltzek","sequence":"additional","affiliation":[{"name":"Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7098-731X","authenticated-orcid":false,"given":"Juan M.","family":"Campos Krauer","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-4518","authenticated-orcid":false,"given":"Samantha M.","family":"Wisely","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"},{"name":"Department of Wildlife Ecology and Conservation, College Agricultural and Life Sciences, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuttichantran","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"},{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4069-0539","authenticated-orcid":false,"given":"John A.","family":"Lednicky","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA"},{"name":"Emerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"ref_1","unstructured":"United States Department of Agriculture, and National Agricultural Statistics Service (2024). 2022 Census of Agriculture."},{"key":"ref_2","unstructured":"(2024, November 15). 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