{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T14:33:56Z","timestamp":1792247636585,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Scientific and Technological Research Fund (FONCYT)","doi-asserted-by":"publisher","award":["PICT-2021-I-A-01199"],"award-info":[{"award-number":["PICT-2021-I-A-01199"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Scientific and Technological Research Fund (FONCYT)","doi-asserted-by":"publisher","award":["PIP 2022-2024"],"award-info":[{"award-number":["PIP 2022-2024"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Scientific and Technological Research Fund (FONCYT)","doi-asserted-by":"publisher","award":["11220210100665"],"award-info":[{"award-number":["11220210100665"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Agency for the Promotion of Research, Technological Development and Innovation","award":["PICT-2021-I-A-01199"],"award-info":[{"award-number":["PICT-2021-I-A-01199"]}]},{"name":"National Agency for the Promotion of Research, Technological Development and Innovation","award":["PIP 2022-2024"],"award-info":[{"award-number":["PIP 2022-2024"]}]},{"name":"National Agency for the Promotion of Research, Technological Development and Innovation","award":["11220210100665"],"award-info":[{"award-number":["11220210100665"]}]},{"name":"Infectious Diseases Government Institute","award":["PICT-2021-I-A-01199"],"award-info":[{"award-number":["PICT-2021-I-A-01199"]}]},{"name":"Infectious Diseases Government Institute","award":["PIP 2022-2024"],"award-info":[{"award-number":["PIP 2022-2024"]}]},{"name":"Infectious Diseases Government Institute","award":["11220210100665"],"award-info":[{"award-number":["11220210100665"]}]},{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)","award":["PICT-2021-I-A-01199"],"award-info":[{"award-number":["PICT-2021-I-A-01199"]}]},{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)","award":["PIP 2022-2024"],"award-info":[{"award-number":["PIP 2022-2024"]}]},{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)","award":["11220210100665"],"award-info":[{"award-number":["11220210100665"]}]},{"name":"Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud \u201cDr. Carlos G. Malbr\u00e1n\u201d","award":["PICT-2021-I-A-01199"],"award-info":[{"award-number":["PICT-2021-I-A-01199"]}]},{"name":"Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud \u201cDr. Carlos G. Malbr\u00e1n\u201d","award":["PIP 2022-2024"],"award-info":[{"award-number":["PIP 2022-2024"]}]},{"name":"Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud \u201cDr. Carlos G. Malbr\u00e1n\u201d","award":["11220210100665"],"award-info":[{"award-number":["11220210100665"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In this work, we performed the genetic characterization of a new variant of orthohantavirus associated with a fatal case of hantavirus pulmonary syndrome, outside the known endemic region, in northwestern Argentina. We first confirmed an orthohantavirus infection by ELISA, testing for the detection of IgM and IgG antibodies. Then, we extracted RNA from 100 microliters of serum, the only sample available, followed by RT-PCR. The amplicons were sequenced using Sanger and next-generation sequencing technology. We obtained partial sequences of 1253 bp, 799 bp and 1675 bp from the S-, M- and L-segments, respectively, showing low sequence identities with all the previously characterized hantaviruses (10.9%, 13.5% and 15.1% of the divergence, respectively). The phylogenetic analysis showed that this virus belongs to the Orthohantavirus andesense species (ANDV), and among the ANDV-like variants, it is more closely related to the Lechiguanas clade. Similar percentages of divergence were considered sufficient to distinguish AND-like variants in previous works. As the patient had no travel history before the onset of disease was reported, we conducted rodent surveys to confirm the presence of reservoirs. The rodent assemblage was compatible with the transitional zone among different ecoregions (Yungas, Chaco and Monte). Moreover, one of the species captured, Oligoryzomys flavescens, was previously described as a reservoir of hantavirus. This species may either host several variants across its range or encompass a species complex, as proposed by some authors.<\/jats:p>","DOI":"10.3390\/v17050717","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T08:32:29Z","timestamp":1747384349000},"page":"717","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6869-1707","authenticated-orcid":false,"given":"Carla M.","family":"Bellomo","sequence":"first","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1203-7225","authenticated-orcid":false,"given":"Sebastian","family":"Kehl","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3605-4679","authenticated-orcid":false,"given":"Daniel Oscar","family":"Alonso","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5677-3537","authenticated-orcid":false,"given":"Walter","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales-IIET, Universidad Nacional de Salta (UNSa), Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7010-1987","authenticated-orcid":false,"given":"Flavia","family":"Cassinelli","sequence":"additional","affiliation":[{"name":"Instituto de Ecorregiones Andinas (INECOA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Jujuy (UNJu), San Salvador de Jujuy Y4600, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7274-9315","authenticated-orcid":false,"given":"Roc\u00edo Mar\u00eda","family":"Coelho","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Bravo","sequence":"additional","affiliation":[{"name":"Hospital Se\u00f1or Del Milagro, Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7521-6040","authenticated-orcid":false,"given":"Sara","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Energ\u00eda No Convencional (INENCO), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Salta (UNSa), Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Dib","sequence":"additional","affiliation":[{"name":"Hospital Se\u00f1or Del Milagro, Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2077-720X","authenticated-orcid":false,"given":"Natalia","family":"Periolo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepci\u00f3n","family":"Toscano","sequence":"additional","affiliation":[{"name":"Hospital Se\u00f1or Del Milagro, Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3994-1265","authenticated-orcid":false,"given":"Jos\u00e9","family":"Gil","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Energ\u00eda No Convencional (INENCO), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Salta (UNSa), Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9362-4933","authenticated-orcid":false,"given":"Francisco","family":"Garc\u00eda Campos","sequence":"additional","affiliation":[{"name":"Direcci\u00f3n General de Coordinaci\u00f3n Epidemiol\u00f3gica, Ministerio de Salud P\u00fablica de Salta, Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7022-4606","authenticated-orcid":false,"given":"Ignacio","family":"Ferro","sequence":"additional","affiliation":[{"name":"Instituto de Ecorregiones Andinas (INECOA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Jujuy (UNJu), San Salvador de Jujuy Y4600, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4356-8136","authenticated-orcid":false,"given":"Valeria Paula","family":"Martinez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Infecciosas (INEI)\u2014Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/viro.1996.0305","article-title":"Genetic identification of a new hantavirus causing severe pulmonary syndrome in Argentina","volume":"220","author":"Padula","year":"1996","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1128\/JCM.38.8.3029-3035.2000","article-title":"Genetic diversity, distribution, and serological features of hantavirus infection in five countries in South America","volume":"38","author":"Padula","year":"2000","journal-title":"J. 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