{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T18:39:53Z","timestamp":1782412793019,"version":"3.54.5"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Temasek Life Sciences Laboratory, Singapore"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Infectious spleen and kidney necrosis virus (ISKNV) is an emerging viral pathogen with an expanding host range, posing a significant threat to economically important fish species. In this study, we isolated the ISKNV strain responsible for disease outbreaks in Asian seabass (Lates calcarifer) and analyzed the transcriptomic profile of spleen tissues from experimentally infected fish. The phylogenetic analysis confirmed that the virus belongs to clade I of ISKNV. Next-generation sequencing identified differentially expressed genes, providing a comprehensive overview of the transcriptional landscape in the spleen of ISKNV-infected fish. The pathway analysis revealed complex host\u2013virus interactions, impacting immune regulation, endocytosis, cell communication, cell cycle arrest, and programmed cell death. To further investigate these interactions, we analyzed relevant pathways in the Reactome database for Asian seabass, humans, and zebrafish, constructed a protein\u2013protein interaction (PPI) network using STRING database, and identified hub genes using six different algorithms. This analysis revealed 69 key genes, including 41 hub genes and 28 key genes that connect different pathways or clusters within the PPI network. These findings provide new insights into the molecular mechanisms driving ISKNV infection in Asian seabass. Future research should focus on elucidating the regulatory functions of these key genes and their roles in ISKNV pathogenesis.<\/jats:p>","DOI":"10.3390\/v17050728","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T06:54:28Z","timestamp":1747724068000},"page":"728","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptomic Analysis of the Spleen from Asian Seabass (Lates calcarifer) Infected with Infectious Spleen and Kidney Necrosis Virus"],"prefix":"10.3390","volume":"17","author":[{"given":"Hong-Yi","family":"Xin","sequence":"first","affiliation":[{"name":"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lim Xin","family":"Ying","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2077-6602","authenticated-orcid":false,"given":"Lee Ching Pei","family":"Carmen","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9816-5908","authenticated-orcid":false,"given":"Mookkan","family":"Prabakaran","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s42003-024-05919-x","article-title":"Hypermethylated Genome of a Fish Vertebrate Iridovirus ISKNV Plays Important Roles in Viral Infection","volume":"7","author":"Liang","year":"2024","journal-title":"Commun. 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