{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:30:14Z","timestamp":1791469814070,"version":"4.3.4"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LH2022C037"],"award-info":[{"award-number":["LH2022C037"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>First reported in 1987, the porcine reproductive and respiratory syndrome virus (PRRSV) has significantly disrupted the major regions affected by PRRSV in the pig breeding industry. Recently, outbreaks of disease caused by recombinant PRRSV strains in China have raised serious concerns. Effective immunization and infection control in pig populations is critical, as the virus frequently undergoes mutation and recombination. This study characterized a novel recombinant PRRSV strain, BX\/CH\/22, isolated from Northeast China. Genetic analysis revealed that BX\/CH\/22 is a recombinant of JXA1, NADC 30-like, and NADC 34-like strains. Phylogenetic analysis of the non-structural protein (NSP) 2 region classified BX\/CH\/22 as JXA1 PRRSV-like, with a characteristic deletion of 30 discontinuous amino acids in NSP2. However, Open Reading Frame (ORF) 5 analysis classified it as NADC 30-like PPRSV, while whole-genome phylogenetic analysis classified it as NADC 34-like PPRSV. Recombination analysis revealed that BX\/CH\/22 contains an NADC 34-like PRRSV backbone, an NSP-coding region from NADC 30-like PRRSV, and an ORF2-ORF6 region from NADC 34-like PRRSV. The strain was isolated from serum samples obtained from commercial swine farms undergoing active PRRS outbreaks. In animal experiments, all BX\/CH\/22-challenged piglets exhibited persistent fever, with peak temperatures &gt;40.5 \u00b0C at 4\u20139 dpi resolving by 11 dpi, accompanied by cough, anorexia, and lethargy. A significant reduction in daily weight gain was observed in infected groups compared to asymptomatic controls, with a 100% survival rate. Our findings provide early warning for PRRSV immune control strategies.<\/jats:p>","DOI":"10.3390\/v17050729","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T06:54:28Z","timestamp":1747724068000},"page":"729","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and Pathogenicity of a Natural Recombinant Pig Reproductive and Respiratory Syndrome Virus in Northeast China"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7607-5994","authenticated-orcid":false,"given":"Zhixin","family":"Tian","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luxiang","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dexin","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqi","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingzhi","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiechao","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"},{"name":"Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"},{"name":"Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.virol.2015.02.012","article-title":"PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity","volume":"479\u2013480","author":"Kappes","year":"2015","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Han, G., Xu, H., Wang, K., and He, F. 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