{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:56:48Z","timestamp":1793041008144,"version":"4.5.0"},"reference-count":59,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 2021 Aplicados Cat I 0039 and grant PICT 2020 Serie A 01012"],"award-info":[{"award-number":["PICT 2021 Aplicados Cat I 0039 and grant PICT 2020 Serie A 01012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet-rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-\u03b1 gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate\u2013early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFN-\u03b3 expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.<\/jats:p>","DOI":"10.3390\/v17060744","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T06:16:03Z","timestamp":1747980963000},"page":"744","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma"],"prefix":"10.3390","volume":"17","author":[{"given":"Sof\u00eda","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2788-3523","authenticated-orcid":false,"given":"Ignacio","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Division of Ruminant Medicine, Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O. Box 7054, 756 51 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Andreoli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari, Universit\u00e0 di Torino, Largo Paolo Braccini, 2, 10095 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6748-3658","authenticated-orcid":false,"given":"S.","family":"Delgado","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Perez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Centro de Investigaci\u00f3n Veterinaria de Tandil (CIVETAN), CONICET-UNCPBA-CICPBA, Campus Universitario, Tandil B7000GHG, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pereyra","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Romeo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4380-5725","authenticated-orcid":false,"given":"S.","family":"Grolli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Medico Veterinarie, Universit\u00e0 di Parma, Via del Taglio 8, 43126 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-7331","authenticated-orcid":false,"given":"Andrea Elizabeth","family":"Verna","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.3168\/jds.S0022-0302(02)74303-8","article-title":"The effect of treatment of clinical endometritis on reproductive performance in dairy cows","volume":"85","author":"LeBlanc","year":"2002","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.fertnstert.2008.12.131","article-title":"Chronic endometritis is a frequent finding in women with recurrent implantation failure after in vitro fertilization","volume":"93","author":"Hartnett","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.anireprosci.2010.07.006","article-title":"Subclinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina","volume":"122","author":"Madoz","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"210","DOI":"10.3168\/jds.2012-5682","article-title":"Clinical endometritis in an Argentinean herd of dairy cows: Risk factors and reproductive efficiency","volume":"96","author":"Giuliodori","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Novikov, A., Breton, A., and Caroff, M. (2017). Micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Microbial Toxins, Methods in Molecular Biology; Springer.","DOI":"10.1007\/978-1-4939-6958-6_16"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.theriogenology.2007.04.056","article-title":"The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow","volume":"68","author":"Williams","year":"2007","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sheldon, I.M., and Roberts, M.H. (2010). Toll-like receptor 4 mediates the response of epithelial and stromal cells to lipopolysaccharide in the endometrium. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0012906"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00705-008-0278-4","article-title":"The order Herpesvirales","volume":"154","author":"Davison","year":"2009","journal-title":"Arch. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jviromet.2003.11.019","article-title":"Activation of bovine herpesvirus 4 lytic replication in a nonpermissive cell line by overexpression of BoHV-4 immediate early (IE) 2 gene","volume":"116","author":"Donofrio","year":"2004","journal-title":"J. Virol. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1128\/JCM.36.7.2109-2111.1998","article-title":"Replication of bovine herpesvirus type 4 in human cells in vitro","volume":"36","author":"Egyed","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9463","DOI":"10.1158\/0008-5472.CAN-05-1076","article-title":"Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo","volume":"65","author":"Gillet","year":"2005","journal-title":"Cancer Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1530\/REP-07-0065","article-title":"Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function","volume":"134","author":"Donofrio","year":"2007","journal-title":"Reproduction"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1530\/REP-08-0171","article-title":"Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: A new insight into bacterial and viral interaction for uterine disease","volume":"136","author":"Donofrio","year":"2008","journal-title":"Reproduction"},{"key":"ref_14","first-page":"87","article-title":"The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd","volume":"57","author":"Gungor","year":"2010","journal-title":"Ank. Univ. Vet. Fak. Derg."},{"key":"ref_15","first-page":"206","article-title":"The investigation of the herpesviruses (BoHV-1 and BoHV-4) on the occurrence of the reproductive disorders in dairy cattle herds, Turkey","volume":"163","author":"Gungor","year":"2012","journal-title":"Rev. Med. Vet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4668","DOI":"10.1128\/JCM.38.12.4668-4671.2000","article-title":"Detection of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk of dairy cattle with history of BoHV-4 infection","volume":"38","author":"Donofrio","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1292\/jvms.68.189","article-title":"Characterization of bovine herpesvirus type 4 isolated from cattle with mastitis and subclinical infection by the virus among cattle","volume":"68","author":"Izumi","year":"2006","journal-title":"J. Vet. Med. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1292\/jvms.66.457","article-title":"Mammary lesions associated with bovine herpesvirus type 4 in a cow with clinical mastitis","volume":"66","author":"Miyano","year":"2004","journal-title":"J. Vet. Med. Sci."},{"key":"ref_19","first-page":"53","article-title":"First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis","volume":"30","author":"Nikolin","year":"2007","journal-title":"New Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2012.04.039","article-title":"Genomic analysis of bovine herpesvirus type 4 (BoHV-4) from Argentina: High genetic variability and novel phylogenetic groups","volume":"160","author":"Verna","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Romeo, F., Spetter, M.J., Moran, P., Pereyra, S., Odeon, A., Perez, S.E., and Verna, A.E. (2020). Analysis of the transcripts encoding for antigenic proteins of bovine gammaherpesvirus 4. J. Vet. Sci., 21.","DOI":"10.4142\/jvs.2020.21.e5"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/s00705-020-04898-8","article-title":"Gene expression and in vitro replication of bovine gammaherpesvirus type 4","volume":"166","author":"Romeo","year":"2021","journal-title":"Arch. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1099\/0022-1317-71-3-647","article-title":"Neutralization of bovine herpesvirus type 4 by pairs of monoclonal antibodies raised against two glycoproteins and identification of antigenic determinants involved in neutralization","volume":"71","author":"Dubuisson","year":"1990","journal-title":"J. Gen. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Davies, D., Meade, K.G., Herath, S., Eckersall, P.D., Gonzalez, D., O White, J., Conlan, R.S., O'Farrelly, C., and Sheldon, I.M. (2008). Toll-like receptor and antimicrobial peptide expression in the bovine endometrium. Reprod. Biol. Endocrinol., 6.","DOI":"10.1186\/1477-7827-6-53"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jri.2005.09.007","article-title":"Use of the cow as a large animal model of uterine infection and immunity","volume":"69","author":"Herath","year":"2006","journal-title":"J. Reprod. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.vetmic.2010.09.031","article-title":"Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U","volume":"148","author":"Franceschi","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1095\/biolreprod.110.086074","article-title":"The chemokine IL-8 is upregulated in bovine endometrial stromal cells by the BoHV-4 IE-2 gene product, ORF50\/Rta: A step ahead toward a mechanism for BoHV-4 induced endometritis","volume":"83","author":"Donofrio","year":"2010","journal-title":"Biol. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Jacca, S., Franceschi, V., Agosti, M., Cavirani, S., Mistretta, F., and Donofrio, G. (2014). Interferon gamma-mediated BoHV-4 replication restriction in bovine endometrial stromal cells is host IDO1 gene expression independent and BoHV-4 IE-2 gene expression dependent. Biol. Reprod., 91.","DOI":"10.1095\/biolreprod.114.123000"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1095\/biolreprod.109.077370","article-title":"Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle","volume":"81","author":"Sheldon","year":"2009","journal-title":"Biol. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1439-0442.2004.00618.x","article-title":"Pregnancy rate following postinsemination intrauterine treatment of endometritis in dairy cattle","volume":"51","author":"Shirazi","year":"2004","journal-title":"J. Vet. Med. Ser. A Physiol. Pathol. Clin. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/vr.146.12.338","article-title":"Effect of three programmes for the treatment of endometritis on the reproductive performance of a dairy herd","volume":"146","author":"Heuwieser","year":"2000","journal-title":"Vet. Rec."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1046\/j.1439-0442.2000.00324.x","article-title":"Reproductive Efficiency of Cows with Endometritis after Treatment with Intrauterine Infusions or Prostaglandin Injections, or No Treatment","volume":"47","author":"Knutti","year":"2000","journal-title":"J. Vet. Med. Ser. A Physiol. Pathol. Clin. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Everts, P., Onishi, K., Jayaram, P., Lana, J.F., and Mautner, K. (2020). Platelet-rich plasma: New performance understandings and therapeutic considerations in 2020. Int. J. Mol. Sci., 21.","DOI":"10.20944\/preprints202010.0069.v1"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1097\/00008505-200110000-00002","article-title":"Platelet-rich plasma (PRP): What is PRP and what is not PRP?","volume":"10","author":"Marx","year":"2001","journal-title":"Implant. Dent."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1126\/science.328.5978.562","article-title":"Beyond clotting: The powers of platelets","volume":"328","author":"Leslie","year":"2010","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Gonz\u00e1lez, D.J., M\u00e9ndez-Bolaina, E., and Trejo-Bahena, N.I. (2012). Platelet-rich plasma peptides: Key for regeneration. Int. J. Pept., 2012.","DOI":"10.1155\/2012\/532519"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Iacopetti, I., Patruno, M., Melotti, L., Martinello, T., Bedin, S., Badon, T., Righetto, E.M., and Perazzi, A. (2020). Autologous platelet-rich plasma enhances the healing of large cutaneous wounds in dogs. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.575449"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Romeo, F., Uriarte, E.L., Delgado, S., Gonz\u00e1lez-Altamiranda, E., Pereyra, S., Mor\u00e1n, P., Ode\u00f3n, A., P\u00e9rez, S., and Verna, A. (2021). Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral coinfection. J. Virol. Methods, 291.","DOI":"10.1016\/j.jviromet.2021.114097"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1007\/s00705-019-04507-3","article-title":"Characterization of the first bovine gammaherpesvirus 4 strain isolated from an aborted bovine fetus in Argentina","volume":"165","author":"Florencia","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s42770-020-00410-1","article-title":"Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: A preliminary study","volume":"52","author":"Spetter","year":"2021","journal-title":"Braz. J. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Palagiano, P., Graziano, L., Scarabello, W., Berni, P., Andreoli, V., and Grolli, S. (2023). Platelet-Rich Plasma Treatment Supported by Ultrasound Detection of Septa in Recurrent Canine Aural Hematoma: A Case Series. Animals, 13.","DOI":"10.3390\/ani13152456"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tvjl.2007.12.031","article-title":"Uterine diseases in cattle after parturition","volume":"176","author":"Sheldon","year":"2008","journal-title":"Vet. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1017\/S1751731108002413","article-title":"Effect of Escherichia coli infection of the bovine uterus from the whole animal to the cell","volume":"2","author":"Williams","year":"2008","journal-title":"Animal"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.repbio.2017.05.006","article-title":"Bovine herpes virus type 4 alters TNF-\u03b1 and IL-8 profiles and impairs the survival of bovine endometrial epithelial cells","volume":"17","author":"Chanrot","year":"2017","journal-title":"Reprod. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.plefa.2018.01.004","article-title":"PGE2 downregulates LPS-induced inflammatory responses via the TLR4-NF-\u03baB signalling pathway in bovine endometrial epithelial cells","volume":"129","author":"Shen","year":"2018","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1530\/rep.0.1230837","article-title":"Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle","volume":"123","author":"Sheldon","year":"2002","journal-title":"Reproduction"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.anireprosci.2004.04.006","article-title":"Postpartum uterine health in cattle","volume":"82\u201383","author":"Sheldon","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"ref_49","first-page":"54","article-title":"Primera evidencia virol\u00f3gica de herpesvirus bovino tipo 4 (BoHV-4) en Argentina","volume":"40","author":"Verna","year":"2008","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1128\/JCM.43.7.3421-3426.2005","article-title":"Potential secondary pathogenic role for bovine herpesvirus 4","volume":"43","author":"Donofrio","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Tebaldi, G., Jacca, S., Montanini, B., Capra, E., Rosamilia, A., Sala, A., Stella, A., Castiglioni, B., Ottonello, S., and Donofrio, G. (2016). Virus-mediated metalloproteinase 1 induction revealed by transcriptome profiling of bovine herpesvirus 4-infected bovine endometrial stromal cells. Biol. Reprod., 95.","DOI":"10.1095\/biolreprod.116.139097"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.3168\/jds.S0022-0302(02)74302-6","article-title":"Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows","volume":"85","author":"LeBlanc","year":"2002","journal-title":"J. Dairy. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/j.1751-0813.2009.00469.x","article-title":"Comparison of two methods of detecting purulent vaginal discharge in postpartum dairy cows and effect of intrauterine cephapirin on reproductive performance","volume":"87","author":"Runciman","year":"2009","journal-title":"Aust. Vet. J."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Xiong, T., Zheng, X., Zhang, K., Wu, H., Dong, Y., Zhou, F., Cheng, B., Li, L., Xu, W., and Su, J. (2022). Ganluyin ameliorates DSS-induced ulcerative colitis by inhibiting the enteric-origin LPS\/TLR4\/NF-\u03baB pathway. J. Ethnopharmacol., 289.","DOI":"10.1016\/j.jep.2022.115001"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.vetimm.2012.09.039","article-title":"Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signalling molecules expression in bovine endometrial epithelial cells","volume":"151","author":"Fu","year":"2013","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Liu, X., Wang, Y., Wen, X., Hao, C., Ma, J., and Yan, L. (2024). Platelet rich plasma alleviates endometritis induced by lipopolysaccharide in mice by inhibiting TLR4\/NF-\u03baB signalling pathway. Am. J. Reprod. Immunol., 91.","DOI":"10.1111\/aji.13833"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1210\/en.2005-1113","article-title":"Expression and function of toll-like receptor 4 in the endometrial cells of the uterus","volume":"147","author":"Herath","year":"2006","journal-title":"Endocrinology"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Donofrio, G., Franceschi, V., Capocefalo, A., Cavirani, S., and Sheldon, I.M. (2009). Isolation and characterization of bovine herpesvirus 4 (BoHV-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome. Reprod. Biol. Endocrinol., 7.","DOI":"10.1186\/1477-7827-7-83"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Jacca, S., Franceschi, V., Colagiorgi, A., Sheldon, M., and Donofrio, G. (2013). Bovine endometrial stromal cells support tumor necrosis factor alpha-induced bovine herpesvirus type 4 enhanced replication. Biol. Reprod., 88.","DOI":"10.1095\/biolreprod.112.106740"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/6\/744\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:38:51Z","timestamp":1760031531000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/6\/744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,23]]},"references-count":59,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["v17060744"],"URL":"https:\/\/doi.org\/10.3390\/v17060744","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,23]]}}}