{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:26:56Z","timestamp":1790148416227,"version":"4.0.1"},"reference-count":101,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rudolf Geigy\u2013Stiftung (RGS)","award":["200902"],"award-info":[{"award-number":["200902"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In Tanzania, dengue outbreaks have occurred almost annually over the past decade, with each new outbreak becoming more severe. This study investigated the prevalence of dengue virus (DENV) serotypes in the wild Aedes aegypti and their blood sources to determine human exposure risk in Dar es Salaam, Tanzania. A two-year longitudinal survey was conducted in the Ilala, Kinondoni, and Temeke districts of Dar es Salaam to sample Ae. aegypti mosquitoes using Biogents Sentinel trap (BGS), Prokopack aspiration, and Gravid Aedes trap (GAT). Collected mosquitoes were pooled in groups of 10 and tested for DENV1\u20134 serotypes using reverse transcription polymerase chain reaction (RT-qPCR). Blood meal sources were identified using an enzyme-linked immunosorbent assay (ELISA). Of 854 tested pools, only DENV-2 was detected and was found in all three districts: Temeke (3\/371 pools), Ilala (1\/206 pools), and Kinondoni (1\/277 pools). Blood meal analysis showed a strong preference for humans (81%) as well as for mixed blood meals that contained human blood and other hosts (17%). Out of 354 collected hosts seeking Ae. aegypti, 78.5% were captured outdoors and 21.5% indoors. This study confirms the circulation of DENV-2 in Ae. aegypti populations, indicating a potential dengue outbreak risk in Tanzania. This study also demonstrates that xenomonitoring may be feasible in this setting. The mosquitoes\u2019 strong preference for human hosts and predominance in outdoor settings pose challenges for dengue control efforts.<\/jats:p>","DOI":"10.3390\/v17060818","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T08:34:32Z","timestamp":1749112472000},"page":"818","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["DENV-2 Circulation and Host Preference Among Highly Anthropophilic, Outdoor-Biting Aedes aegypti in Dar es Salaam, Tanzania"],"prefix":"10.3390","volume":"17","author":[{"given":"Frank S. C.","family":"Tenywa","sequence":"first","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"},{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvan","family":"H\u00e4lg","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haji","family":"Makame","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Moore","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"},{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osward","family":"Dogan","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harubu I.","family":"Mapipi","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7191-7305","authenticated-orcid":false,"given":"Jane J.","family":"Machange","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasoro S.","family":"Lilolime","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0906-2449","authenticated-orcid":false,"given":"Lorenz M.","family":"Hofer","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis D.","family":"Batao","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tunu G.","family":"Mwamlima","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0181-5781","authenticated-orcid":false,"given":"Pie","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-6654","authenticated-orcid":false,"given":"Sarah J.","family":"Moore","sequence":"additional","affiliation":[{"name":"Vector Control Product Testing Unit, Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Bagamoyo P.O. Box 74, Tanzania"},{"name":"Swiss Tropical and Public Health Institute, Kreuzstrasse 2, Allschwil, 4123 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, 4001 Basel, Switzerland"},{"name":"School of Life Sciences and Bioengineering, The Nelson Mandela African Institution of Science and Technology (NM-AIST), Arusha P.O. Box 447, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S40","DOI":"10.1016\/j.ijantimicag.2010.06.018","article-title":"Update on the global spread of dengue","volume":"36","author":"Guzman","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Brady, O.J., Gething, P.W., Bhatt, S., Messina, J.P., Brownstein, J.S., Hoen, A.G., Moyes, C.L., Farlow, A.W., Scott, T.W., and Hay, S.I. (2012). Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001760"},{"key":"ref_3","unstructured":"European Centre for Disease Prevention and Control (2024, January 29). Dengue Cases January\u2013December 2023. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/dengue-cases-january-december-2023."},{"key":"ref_4","unstructured":"World Health Organization (2024, May 30). Dengue-Global Situation. Available online: https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2024-DON518."},{"key":"ref_5","unstructured":"European Centre for Disease Prevention and Control (2025, February 03). Dengue Worldwide Overview. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/twelve-month-dengue-virus-disease-case-notification-rate-100-000-population?utm_source=chatgpt.com."},{"key":"ref_6","unstructured":"World Health Organization (2023, April 23). Dengue and Severe Dengue. WHO. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs117\/en\/."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"A distribui\u00e7\u00e3o global e o \u00f4nus da dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1093\/cid\/ciw589","article-title":"Viremia and clinical presentation in Nicaraguan patients infected with Zika virus, chikungunya virus, and dengue virus","volume":"63","author":"Waggoner","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","unstructured":"World Health Organization (2023, April 23). Dengue\u2014Global Situation. Available online: https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2023-DON498."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Boillat-Blanco, N., Klaassen, B., Mbarack, Z., Samaka, J., Mlaganile, T., Masimba, J., Franco Narvaez, L., Mamin, A., Genton, B., and Kaiser, L. (2018). Dengue fever in Dar es Salaam, Tanzania: Clinical features and outcome in populations of black and non-black racial category. BMC Infect. Dis., 18.","DOI":"10.1186\/s12879-018-3549-z"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0140-6736(14)60572-9","article-title":"Dengue","volume":"385","author":"Guzman","year":"2015","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1038\/s41564-019-0476-8","article-title":"The current and future global distribution and population at risk of dengue","volume":"4","author":"Messina","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bennett, S.N. (2014). 17 Taxonomy and Evolutionary Relationships of Flaviviruses. Dengue and Dengue Hemorrhagic Fever, CABI.","DOI":"10.1079\/9781845939649.0322"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1038\/nrmicro2460","article-title":"Dengue: A continuing global threat","volume":"8","author":"Guzman","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Harapan, H., Michie, A., Sasmono, R.T., and Imrie, A. (2020). Dengue: A minireview. Viruses, 12.","DOI":"10.3390\/v12080829"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"012019","DOI":"10.1088\/1742-6596\/1358\/1\/012019","article-title":"Genotypes of the dengue virus in patients with dengue infection from Sabah, Malaysia","volume":"1358","author":"Najri","year":"2019","journal-title":"Proc. J. Phys. Conf. Ser."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S1567-1348(03)00004-2","article-title":"The origin, emergence and evolutionary genetics of dengue virus","volume":"3","author":"Holmes","year":"2003","journal-title":"Infect. Genet. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.meegid.2015.03.006","article-title":"Genomic analysis and growth characteristic of dengue viruses from Makassar, Indonesia","volume":"32","author":"Sasmono","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1007\/s00018-010-0357-z","article-title":"Dengue virus life cycle: Viral and host factors modulating infectivity","volume":"67","author":"Wilschut","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s00705-013-1645-3","article-title":"Secondary infection as a risk factor for dengue hemorrhagic fever\/dengue shock syndrome: An historical perspective and role of antibody-dependent enhancement of infection","volume":"158","author":"Guzman","year":"2013","journal-title":"Arch. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16055","DOI":"10.1038\/nrdp.2016.55","article-title":"Dengue infection","volume":"2","author":"Guzman","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1086\/315215","article-title":"Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity","volume":"181","author":"Vaughn","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1099\/vir.0.060384-0","article-title":"Demonstration of marmosets (Callithrix jacchus) as a non-human primate model for secondary dengue virus infection: High levels of viraemia and serotype cross-reactive antibody responses consistent with secondary infection of humans","volume":"95","author":"Moi","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_25","first-page":"1349","article-title":"Dengue virus infection in Africa","volume":"17","author":"Amarasinghe","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","unstructured":"Sang, R.C. (2007). Dengue in Africa. Report of the Scientific Working Group Meeting on Dengue, WHO Special Programme for Research and Training in Tropical Diseases."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chipwaza, B., Mugasa, J.P., Selemani, M., Amuri, M., Mosha, F., Ngatunga, S.D., and Gwakisa, P.S. (2014). Dengue and Chikungunya fever among viral diseases in outpatient febrile children in Kilosa district hospital, Tanzania. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0003335"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"20730","DOI":"10.2807\/1560-7917.ES2014.19.10.20730","article-title":"Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014","volume":"19","author":"Parreira","year":"2014","journal-title":"Eurosurveillance"},{"key":"ref_29","first-page":"19541","article-title":"Dengue type 3 virus infections in European travellers returning from the Comoros and Zanzibar, February\u2013April 2010","volume":"15","author":"Gautret","year":"2010","journal-title":"Eurosurveillance"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.4269\/ajtmh.1979.28.1045","article-title":"Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti","volume":"28","author":"Gubler","year":"1979","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"603","DOI":"10.4269\/ajtmh.1985.34.603","article-title":"Comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and Japanese encephalitis viruses","volume":"34","author":"Rosen","year":"1985","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"539","DOI":"10.4269\/ajtmh.2003.68.539","article-title":"Efficiency of dengue serotype 2 virus strains to infect and disseminate in Aedes aegypti","volume":"68","author":"Armstrong","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ijid.2017.11.026","article-title":"Global risk mapping for major diseases transmitted by Aedes aegypti and Aedes albopictus","volume":"67","author":"Leta","year":"2018","journal-title":"Int. J. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1146\/annurev-ento-011019-024918","article-title":"The Global Expansion of Dengue: How Aedes aegypti Mosquitoes Enabled the First Pandemic Arbovirus","volume":"65","author":"Brady","year":"2020","journal-title":"Annu. Rev. Entomol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1056\/NEJMoa1800820","article-title":"Effect of dengue serostatus on dengue vaccine safety and efficacy","volume":"379","author":"Sridhar","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/jmedent\/42.5.844","article-title":"Blood feeding patterns of Aedes aegypti and Aedes albopictus in Thailand","volume":"42","author":"Ponlawat","year":"2005","journal-title":"J. Med. Entomol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1093\/jme\/tjaa117","article-title":"Feeding habits of vector mosquitoes in Harris County, TX, 2018","volume":"57","author":"Mann","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1093\/trstmh\/trs095","article-title":"Bloodfeeding patterns of sylvatic arbovirus vectors in southeastern Senegal","volume":"107","author":"Diallo","year":"2013","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1007\/s00436-015-4634-5","article-title":"Host-feeding pattern of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in heterogeneous landscapes of South Andaman, Andaman and Nicobar Islands, India","volume":"114","author":"Sivan","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Olson, M.F., Ndeffo-Mbah, M.L., Juarez, J.G., Garcia-Luna, S., Martin, E., Borucki, M.K., Frank, M., Estrada-Franco, J.G., Rodr\u00edguez-P\u00e9rez, M.A., and Fern\u00e1ndez-Santos, N.A. (2020). High rate of non-human feeding by Aedes aegypti reduces Zika virus transmission in South Texas. Viruses, 12.","DOI":"10.3390\/v12040453"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.4269\/ajtmh.21-0508","article-title":"Blood feeding patterns of Aedes aegypti populations in Senegal","volume":"106","author":"Sene","year":"2022","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1093\/jme\/tjaa083","article-title":"Human blood feeding by Aedes aegypti (Diptera: Culicidae) in the Florida Keys and a review of the literature","volume":"57","author":"Pruszynski","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zahid, M.H., Van Wyk, H., Morrison, A.C., Coloma, J., Lee, G.O., Cevallos, V., Ponce, P., and Eisenberg, J.N. (2023). The biting rate of Aedes aegypti and its variability: A systematic review (1970\u20132022). PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0010831"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.actatropica.2019.02.006","article-title":"Surveillance of Aedes aegypti indoors and outdoors using Autocidal Gravid Ovitraps in South Texas during local transmission of Zika virus, 2016 to 2018","volume":"192","author":"Martin","year":"2019","journal-title":"Acta Trop."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s13071-022-05261-3","article-title":"Bionomic aspects of dengue vectors Aedes aegypti and Aedes albopictus at domestic settings in urban, suburban and rural areas in Gampaha District, Western Province of Sri Lanka","volume":"15","author":"Dalpadado","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_46","first-page":"158","article-title":"Landing periodicity of Aedes aegypti with implications for dengue transmission in Trinidad, West Indies","volume":"25","author":"Chadee","year":"2000","journal-title":"J. Vector Ecol. J. Soc. Vector Ecol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e44","DOI":"10.1016\/j.ijid.2011.09.018","article-title":"Seroprevalence of dengue infection: A cross-sectional survey in mainland Tanzania and on Pemba Island, Zanzibar","volume":"16","author":"Vairo","year":"2012","journal-title":"Int. J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3201\/eid2003.130150","article-title":"IgG against dengue virus in healthy blood donors, Zanzibar, Tanzania","volume":"20","author":"Vairo","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.3201\/eid1611.101061","article-title":"Importation of dengue virus type 3 to Japan from Tanzania and C\u00f4te d\u2019Ivoire","volume":"16","author":"Moi","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Chipwaza, B., Sumaye, R.D., Weisser, M., Gingo, W., Yeo, N.K.-W., Amrun, S.N., Okumu, F.O., and Ng, L.F. (2021). Occurrence of 4 dengue virus serotypes and chikungunya virus in Kilombero Valley, Tanzania, during the dengue outbreak in 2018. Open Forum Infectious Diseases, Oxford University Press.","DOI":"10.1101\/2020.10.09.20209783"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"171","DOI":"10.4269\/ajtmh.2012.11-0393","article-title":"Chikungunya and dengue fever among hospitalized febrile patients in northern Tanzania","volume":"86","author":"Hertz","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_52","unstructured":"World Health Organization (2014). Engue Outbreak in the United Republic of Tanzania, World Health Organization."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Mboera, L.E., Mweya, C.N., Rumisha, S.F., Tungu, P.K., Stanley, G., Makange, M.R., Misinzo, G., De Nardo, P., Vairo, F., and Oriyo, N.M. (2016). The risk of dengue virus transmission in Dar es Salaam, Tanzania during an epidemic period of 2014. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004313"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kelly, M.E., Msafiri, F., Affara, M., Gehre, F., Moremi, N., Mghamba, J., Misinzo, G., Thye, T., Gatei, W., and Whistler, T. (2023). Molecular Characterization and Phylogenetic Analysis of Dengue Fever Viruses in Three Outbreaks in Tanzania Between 2017 and 2019. PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011289"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.3201\/eid2509.190814","article-title":"Dengue virus type 1 infection in traveler returning from Tanzania to Japan, 2019","volume":"25","author":"Okada","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1080\/20477724.2021.1905302","article-title":"Co-circulation of Dengue Virus Serotypes 1 and 3 during the 2019 epidemic in Dar es Salaam, Tanzania","volume":"115","author":"Mwanyika","year":"2021","journal-title":"Pathog. Glob. Health"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.ijid.2021.08.040","article-title":"Seroprevalence and associated risk factors of chikungunya, dengue, and Zika in eight districts in Tanzania","volume":"111","author":"Mwanyika","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"ref_58","unstructured":"SACIDS (2019). Dengue Outbreaks in Tanzania: Recent Trends and Importance of Research Data in Disease Surveillance, Southern African Centre for Infectious Disease Surveillance."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3201\/eid2205.151462","article-title":"Clinical, virologic, and epidemiologic characteristics of dengue outbreak, Dar es Salaam, Tanzania, 2014","volume":"22","author":"Vairo","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Shauri, H.S., Ngadaya, E., Senkoro, M., Buza, J.J., and Mfinanga, S. (2021). Seroprevalence of Dengue and Chikungunya antibodies among blood donors in Dar es Salaam and Zanzibar, Tanzania: A cross-sectional study. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06549-y"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Kajeguka, D.C., Kaaya, R.D., Mwakalinga, S., Ndossi, R., Ndaro, A., Chilongola, J.O., Mosha, F.W., Schi\u00f8ler, K.L., Kavishe, R.A., and Alifrangis, M. (2016). Prevalence of dengue and chikungunya virus infections in north-eastern Tanzania: A cross sectional study among participants presenting with malaria-like symptoms. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1511-5"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Mustafa, U.-k., Kreppel, K.S., and Sauli, E. (2024). Dengue virus transmission during non-outbreak period in Dar Es Salaam, Tanzania: A cross-sectional survey. BMC Infect. Dis., 24.","DOI":"10.1186\/s12879-024-10109-5"},{"key":"ref_63","unstructured":"(2022, December 01). National Bureau of Statistics Ministry of Finance and Office of Chief Government Statistician President\u2019s Office, Economy and Development Planning. Population and Housing Census. Administrative units Population Distribution and Age and Sex Distribution Report Tanzania\u2013Volume1a, Available online: https:\/\/sensa.nbs.go.tz\/publication\/volume1a.pdf."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Msellemu, D., Gavana, T., Ngonyani, H., Mlacha, Y.P., Chaki, P., and Moore, S.J. (2020). Knowledge, attitudes and bite prevention practices and estimation of productivity of vector breeding sites using a Habitat Suitability Score (HSS) among households with confirmed dengue in the 2014 outbreak in Dar es Salaam, Tanzania. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0007278"},{"key":"ref_65","unstructured":"(2022, December 01). Tanzania Metrology Agency (TMA), Available online: http:\/\/www.meteo.go.tz."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Machange, J.J., Maasayi, M.S., Mundi, J., Moore, J., Muganga, J.B., Odufuwa, O.G., Moore, S.J., and Tenywa, F.C. (2024). Comparison of the Trapping Efficacy of Locally Modified Gravid Aedes Trap and Autocidal Gravid Ovitrap for the Monitoring and Surveillance of Aedes aegypti Mosquitoes in Tanzania. Insects, 15.","DOI":"10.20944\/preprints202404.0764.v1"},{"key":"ref_67","unstructured":"Wilkerson, R.C., Linton, Y.-M., and Strickman, D. (2021). Mosquitoes of the World, Johns Hopkins University Press."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M.W., and Shipley, G.L. (2009). The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments, Oxford University Press.","DOI":"10.1373\/clinchem.2008.112797"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1590\/0074-02760150363","article-title":"Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia","volume":"111","author":"Castellanos","year":"2016","journal-title":"Mem. Do Inst. Oswaldo Cruz"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1186\/s13071-020-04470-y","article-title":"Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines","volume":"13","author":"Balingit","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/jmedent\/25.1.9","article-title":"Bloodmeal identification by direct enzyme-linked immunosorbent assay (ELISA), tested on Anopheles (Diptera: Culicidae) in Kenya","volume":"25","author":"Beier","year":"1988","journal-title":"J. Med. Entomol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1177\/15353702231181356","article-title":"Analysis of dengue fever disease in West Africa","volume":"248","author":"Gyasi","year":"2023","journal-title":"Exp. Biol. Med."},{"key":"ref_73","unstructured":"World Health Organization (2019). Dengue and Severe Dengue Fact Sheet, World Health Organization."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Masika, M.M., Korhonen, E.M., Smura, T., Uusitalo, R., Vapalahti, K., Mwaengo, D., J\u00e4\u00e4skel\u00e4inen, A.J., Anzala, O., Vapalahti, O., and Huhtamo, E. (2020). Detection of dengue virus type 2 of Indian origin in acute febrile patients in rural Kenya. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008099"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"veaa026","DOI":"10.1093\/ve\/veaa026","article-title":"Origin and evolution of dengue virus type 2 causing outbreaks in Kenya: Evidence of circulation of two cosmopolitan genotype lineages","volume":"6","author":"Langat","year":"2020","journal-title":"Virus Evol."},{"key":"ref_76","unstructured":"African Development Bank (2022, December 01). Urbanization in Africa. Available online: https:\/\/www.afdb.org\/fileadmin\/uploads\/afdb\/Documents\/Publications\/African_Development_Report_2012.pdf."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Rachmawati, Y., Ekawardhani, S., Fauziah, N., Faridah, L., and Watanabe, K. (2024). Potential Way to Develop Dengue Virus Detection in Aedes Larvae as an Alternative for Dengue Active Surveillance: A Literature Review. Trop. Med. Infect. Dis., 9.","DOI":"10.3390\/tropicalmed9030060"},{"key":"ref_78","unstructured":"World Health Organization (2023). Urban Yellow Fever Risk Management: Preparedness and Response: Handbook for National Operational Planning, World Health Organization."},{"key":"ref_79","first-page":"78","article-title":"Concurrent Infection with Dengue and Chikungunya Viruses in Humans and Mosquitoes: A Field Survey in Lower Moshi, Tanzania","volume":"4","author":"Chilongola","year":"2022","journal-title":"E. Afr. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Joseph, N.K., Mumo, E., Morlighem, C., Macharia, P.M., Snow, R.W., and Linard, C. (2024). Mosquito-borne diseases in urban East African Community region: A scoping review of urban typology research and mosquito genera overlap, 2000\u20132024. Front. Trop. Dis., 5.","DOI":"10.3389\/fitd.2024.1499520"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1093\/jme\/tjae121","article-title":"A numbers game: Mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America","volume":"62","author":"Mojica","year":"2025","journal-title":"J. Med. Entomol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"107226","DOI":"10.1016\/j.ijid.2024.107226","article-title":"Prevalence of dengue, Zika, and chikungunya virus infections among mosquitoes in Asia: A systematic review and meta-analysis","volume":"148","author":"Maneerattanasak","year":"2024","journal-title":"Int. J. Infect. Dis."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"e00264","DOI":"10.1016\/j.parepi.2022.e00264","article-title":"Persistently high proportions of plasmodium-infected Anopheles funestus mosquitoes in two villages in the Kilombero valley, South-Eastern Tanzania","volume":"18","author":"Mapua","year":"2022","journal-title":"Parasite Epidemiol. Control"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1186\/1475-2875-13-331","article-title":"Increasing role of Anopheles funestus and Anopheles arabiensis in malaria transmission in the Kilombero Valley, Tanzania","volume":"13","author":"Lwetoijera","year":"2014","journal-title":"Malar. J."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"753","DOI":"10.2307\/4591271","article-title":"Epidemiologic models in studies of vetor-borne diseases: The re dyer lecture","volume":"76","author":"Macdonald","year":"1961","journal-title":"Public Health Rep."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1093\/jme\/tjab111","article-title":"Resting behavior of blood-fed females and host feeding preferences of Aedes aegypti (Diptera: Culicidae) morphological forms in Senegal","volume":"58","author":"Diouf","year":"2021","journal-title":"J. Med. Entomol."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Badolo, A., Sombi\u00e9, A., Yam\u00e9ogo, F., Wangrawa, D.W., Sanon, A., Pignatelli, P.M., Sanon, A., Viana, M., Kanuka, H., and Weetman, D. (2022). First comprehensive analysis of Aedes aegypti bionomics during an arbovirus outbreak in west Africa: Dengue in Ouagadougou, Burkina Faso, 2016\u20132017. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010059"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"849","DOI":"10.4269\/ajtmh.2009.09-0008","article-title":"Blood sources of mosquitoes collected from urban and peri-urban environments in eastern Australia with species-specific molecular analysis of avian blood meals","volume":"81","author":"Jansen","year":"2009","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1007\/s00436-022-07537-0","article-title":"Blood feeding habits of mosquitoes: Hardly a bite in South America","volume":"121","author":"Cardo","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/0074-0276130395","article-title":"History of domestication and spread of Aedes aegypti-a review","volume":"108","author":"Powell","year":"2013","journal-title":"Mem\u00f3rias Do Inst. Oswaldo Cruz"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4269\/ajtmh.2005.73.445","article-title":"Potential role of sylvatic and domestic African mosquito species in dengue emergence","volume":"73","author":"Diallo","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1186\/s13071-019-3732-0","article-title":"Sylvatic cycles of arboviruses in non-human primates","volume":"12","author":"Valentine","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1186\/1756-3305-7-435","article-title":"Vector competence of Aedes aegypti populations from Kilifi and Nairobi for dengue 2 virus and the influence of temperature","volume":"7","author":"Chepkorir","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_94","first-page":"680","article-title":"Host preferences of various strains of Aedes aegypti and A. simpsoni as determined by an olfactometer","volume":"47","author":"Gouck","year":"1972","journal-title":"Bull. World Health Organ."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00285-002-0168-4","article-title":"Coexistence of different serotypes of dengue virus","volume":"46","author":"Esteva","year":"2003","journal-title":"J. Math. Biol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s002850050064","article-title":"Competitive exclusion in a vector-host model for the dengue fever","volume":"35","author":"Feng","year":"1997","journal-title":"J. Math. Biol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.4269\/ajtmh.24-0240","article-title":"Blood-Feeding Patterns and Resting Behavior of Aedes aegypti from Three Health Districts of Ouagadougou City, Burkina Faso","volume":"111","author":"Yameogo","year":"2024","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1093\/jme\/tjaa024","article-title":"Biting Behavior and Molecular Identification of Aedes aegypti (Diptera: Culicidae) Subspecies in Some Selected Recent Yellow Fever Outbreak Communities in Northern Ghana","volume":"57","author":"Frempong","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Montenegro-Qui\u00f1onez, C.A., Louis, V.R., Horstick, O., Velayudhan, R., Dambach, P., and Runge-Ranzinger, S. (2023). Interventions against Aedes\/dengue at the household level: A systematic review and meta-analysis. eBioMedicine, 93.","DOI":"10.1016\/j.ebiom.2023.104660"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1056\/NEJMoa2030243","article-title":"Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue","volume":"384","author":"Utarini","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Agha, S.B., Tchouassi, D.P., Turell, M.J., Bastos, A.D.S., and Sang, R. (2019). Entomological assessment of dengue virus transmission risk in three urban areas of Kenya. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007686"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/6\/818\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:47:05Z","timestamp":1760032025000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/6\/818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,5]]},"references-count":101,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["v17060818"],"URL":"https:\/\/doi.org\/10.3390\/v17060818","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,5]]}}}