{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:29:22Z","timestamp":1780392562596,"version":"3.54.1"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\/NIDA","award":["R61DA059924"],"award-info":[{"award-number":["R61DA059924"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Psychostimulants such as methamphetamine (Meth) induce high dopamine (DA) levels in the brain, which can modify immune cells expressing DA receptors. This is relevant in conditions of infection with the human immunodeficiency virus (HIV), overlapping with substance use. However, the effects of DA on HIV latency phenotypes are largely unknown. We used single-cell methods and gene network computational analysis to understand these relationships, using the U1 latent promonocyte model to identify signatures of latency and its reversal in the context of DA exposure. Our findings point to mechanisms by which high DA levels in the brains of substance users may impact HIV transcription and neuroinflammation. Our data indicate that latency is maintained along with the expression of histone linkers and components of chromatin organization, with increased metabolic pathways that may lead to pathways in neurodegeneration. DA exposure decreased latency signature genes, histone linkers, and protein-containing complex organization components, unleashing inflammatory pathways and HIV gene transcription. Overall, this work suggests that DA can induce latency reversal through mechanisms that can be harnessed to drive cells. The proposed methods developed here in cell lines can be used to identify latency signatures in other HIV infection systems.<\/jats:p>","DOI":"10.3390\/v17070895","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T07:55:43Z","timestamp":1751442943000},"page":"895","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Single-Cell Perspective on the Effects of Dopamine in the Regulation of HIV Latency Phenotypes in a Myeloid Cell Model"],"prefix":"10.3390","volume":"17","author":[{"given":"Liana V.","family":"Basova","sequence":"first","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4342-9849","authenticated-orcid":false,"given":"Wei Ling","family":"Lim","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4194-4932","authenticated-orcid":false,"given":"Violaine","family":"Delorme-Walker","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tera","family":"Riley","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaylin","family":"Au","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Siqueira","family":"Lima","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"},{"name":"Interdisciplinary Fellowship on neuroAIDS, University of California San Diego, San Diego, CA 92103, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0120-3569","authenticated-orcid":false,"given":"Marina","family":"Lusic","sequence":"additional","affiliation":[{"name":"Heidelberg University Hospital, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4931-752X","authenticated-orcid":false,"given":"Ronald J.","family":"Ellis","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2032-374X","authenticated-orcid":false,"given":"Howard S.","family":"Fox","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4705-372X","authenticated-orcid":false,"given":"Maria Cecilia Garibaldi","family":"Marcondes","sequence":"additional","affiliation":[{"name":"San Diego Biomedical Research Institute, San Diego, CA 92121, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Basova, L., Najera, J.A., Bortell, N., Wang, D., Moya, R., Lindsey, A., Semenova, S., Ellis, R.J., and Marcondes, M.C.G. 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