{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:28:51Z","timestamp":1760059731898,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre (NCN) of Poland","award":["2011\/03\/B\/NZ6\/03711"],"award-info":[{"award-number":["2011\/03\/B\/NZ6\/03711"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Caprine arthritis and encephalitis (CAE), caused by small ruminant lentivirus (SRLV), is a key disease of goats, with chronic inflammation of joints and brain symptoms leading to losses in milk production and animal trade. In this study, we analyzed gene expressions in the milk somatic cells (MSCs) of seropositive (SRLV-SP) and seronegative (SRLV-SN) goats to identify transcriptomic changes using a non-invasive sampling method. Materials and Methods: This study was conducted on goats of two Polish breeds (Polish Improved White and Polish Improved Fawn), which were kept at the Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, during their first lactation. MSCs were isolated from milk, and gene expression was analyzed using the Goat Gene Expression Microarray. The results were verified by RT-qPCR for five genes (DUSP26, PRLR, SCARA3, APBB2, OR4F4). Statistical analysis was performed in GeneSpring 12 software. Results: Microarrays showed reduced expression of DUSP26, PRLR, SCARA3, APBB2, and OR4F4 genes in SRLV-SP goats. RT-qPCR confirmed changes for DUSP26, SCARA3, and APBB2. Functional analysis indicated associations with immune processes and HIV-like pathways. Discussion: The results suggest that SRLV induces transcriptomic perturbations, especially in immunity-related genes. MSCs are an effective model for non-invasive studies, and further studies may support strategies for combating CAE.<\/jats:p>","DOI":"10.3390\/v17070944","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T08:22:07Z","timestamp":1751530927000},"page":"944","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Gene Expression Profile of Milk Somatic Cells of Small Ruminant Lentivirus-Seropositive and -Seronegative Dairy Goats (Capra hircus) During Their First Lactation"],"prefix":"10.3390","volume":"17","author":[{"given":"Joanna","family":"P\u0142awi\u0144ska-Czarnak","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9815-7844","authenticated-orcid":false,"given":"Alicja","family":"Majewska","sequence":"additional","affiliation":[{"name":"Department of Physiology Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3783-6592","authenticated-orcid":false,"given":"Joanna Magdalena","family":"Zarzy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7977-9875","authenticated-orcid":false,"given":"Jaros\u0142aw","family":"Kaba","sequence":"additional","affiliation":[{"name":"Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7193-2006","authenticated-orcid":false,"given":"Emilia","family":"Bagnicka","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.14202\/vetworld.2020.1344-1350","article-title":"The epidemiological background of small ruminant lentivirus infection in goats from Romania","volume":"13","author":"Potarniche","year":"2020","journal-title":"Vet. World"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.cimid.2011.12.003","article-title":"Small ruminant lentiviruses: Immunopathogenesis of visna-maedi and caprine arthritis and encephalitis virus","volume":"35","author":"Blacklaws","year":"2012","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.3390\/v5041175","article-title":"Small Ruminant Lentiviruses: Genetic Variability, Tropism and Diagnosis","volume":"5","author":"Reina","year":"2013","journal-title":"Viruses"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"511","DOI":"10.2478\/jvetres-2022-0071","article-title":"Longitudinal Study on Seroreactivity of Goats Exposed to Colostrum and Milk of Small Ruminant Lentivirus-infected Dams","volume":"66","author":"Kaba","year":"2022","journal-title":"J. Vet. Res."},{"unstructured":"Ax\u00e9n, C., Brytting, M., Bujila, I., Chenais, E., Dryselius, R., Eriksson, H., Forsgren, E., Grant, M., Gr\u00f6ndahl, G., and Hallgren, G. (2020). Surveillance of Infectious Diseases in Animals and Humans in Sweden 2020, National Veterinary Institute (SVA).","key":"ref_5"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.prevetmed.2016.12.012","article-title":"A census to determine the prevalence and risk factors for caprine arthritis-encephalitis virus and visna\/maedi virus in the Swiss goat population","volume":"137","author":"Thomann","year":"2017","journal-title":"Prev. Vet. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1136\/vr.104503","article-title":"Achievements of an eradication programme against caprine arthritis encephalitis virus in South Tyrol, Italy","volume":"182","author":"Tavella","year":"2018","journal-title":"Vet. Rec."},{"unstructured":"Nagel-Alne, G.E. (2015). Healthier Goats Disease Eradication Programme: A Healthy Initiative. [Ph.D. Thesis, Norwegian University of Life Sciences].","key":"ref_8"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"107149","DOI":"10.1016\/j.smallrumres.2023.107149","article-title":"Challenges using serological diagnostics in elimination of visna\/maedi: Serological results from two outbreaks in Norwegian sheep","volume":"229","author":"Jerre","year":"2023","journal-title":"Small Rumin. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.3390\/v5041175","article-title":"Small Ruminant Lentiviruses: Genetic Variability, Tropism and Diagnosis","volume":"5","author":"Reina","year":"2013","journal-title":"Viruses"},{"doi-asserted-by":"crossref","unstructured":"Nalbert, T., Czopowicz, M., Szalu\u015b-Jordanow, O., Witkowski, L., Moroz, A., Mickiewicz, M., Markowska-Daniel, I., S\u0142oniewska, D., Bagnicka, E., and Kaba, J. (2020). The effect of the subclinical small ruminant lentivirus infection of female goats on the growth of kids. PLoS ONE, 15.","key":"ref_11","DOI":"10.1371\/journal.pone.0230617"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.tvjl.2012.12.020","article-title":"Quantitative estimation of the impact of caprine arthritis encephalitis virus infection on milk production by dairy goats","volume":"197","author":"Peris","year":"2013","journal-title":"Vet. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.3168\/jds.2011-4680","article-title":"Twelve-year cohort study on the influence of caprine arthritis-encephalitis virus infection on milk yield and composition","volume":"95","author":"Kaba","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/s13567-018-0607-x","article-title":"Small Ruminant Lentivirus Infection Influences Expression of Acute Phase Proteins and Cathelicidin Genes in Milk Somatic Cells and Peripheral Blood Leukocytes of Dairy Goats","volume":"49","author":"Zalewska","year":"2018","journal-title":"Vet. Res."},{"unstructured":"Strzelecki, J. (2009). Normy \u017cywienia k\u00f3z mlecznych. W: Normy \u017cywienia prze\u017cuwaczy: Warto\u015b\u0107 pokarmowa francuskich i krajowych pasz dla prze\u017cuwaczy [In Polish], Standard of dairy goats\u2019 feeging. Standard of Ruminants\u2019 Feeding: Nutrient Value of French and Domestic Fodders for Ruminants, Research Institute of Animal Production.","key":"ref_15"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1177\/1040638717729397","article-title":"Use of two commercial caprine arthritis-encephalitis immunoenzymatic assays for screening of arthritic goats","volume":"30","author":"Czopowicz","year":"2018","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"605","DOI":"10.2478\/aoas-2019-0024","article-title":"An Optimized Method of RNA Isolation from Goat Milk Somatic Cells for Transcriptomic Analysis, Ann","volume":"19","author":"Bogdan","year":"2019","journal-title":"Anim. Sci."},{"key":"ref_18","first-page":"159","article-title":"Design and implementation of a database enhancing the collection, management and analysis of data in an animal sciences project","volume":"36","author":"Ochnio","year":"2018","journal-title":"Anim. Sci. Pap. Rep."},{"doi-asserted-by":"crossref","unstructured":"P\u0142awi\u0144ska-Czarnak, J., Majewska, A., Zarzy\u0144ska, J., Bogdan, J., Kaba, J., Anusz, K., and Bagnicka, E. (2021). Gene Expression Profile in Peripheral Blood Nuclear Cells of Small Ruminant Lentivirus-Seropositive and Seronegative Dairy Goats in Their First Lactation. Animals, 11.","key":"ref_19","DOI":"10.3390\/ani11040940"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","article-title":"Mfold web server for nucleic acid folding and hybridization prediction","volume":"31","author":"Zuker","year":"2003","journal-title":"Nucleic Acids Res."},{"unstructured":"(2019, May 27). Available online: http:\/\/oligocalc.eu\/.","key":"ref_21"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.smallrumres.2010.08.008","article-title":"Reference gene selection for quantitative real-time PCR normalization: Application in the caprine mammary gland","volume":"95","author":"Finot","year":"2011","journal-title":"Small Rumin. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1080\/21691401.2023.2252872","article-title":"Molecular complexity of mammary glands development: A review of lactogenic differentiation in epithelial cells","volume":"51","author":"Jena","year":"2023","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"doi-asserted-by":"crossref","unstructured":"Ha, J., Kang, E., Seo, J., and Cho, S. (2019). Phosphorylation Dynamics of JNK Signaling: Effects of Dual-Specificity Phosphatases (DUSPs) on the JNK Pathway. Int. J. Mol. Sci., 20.","key":"ref_25","DOI":"10.3390\/ijms20246157"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e17471","DOI":"10.1097\/MD.0000000000017471","article-title":"Scavenger receptor class a, member 3 is associated with severity of hand, foot, and mouth disease in a case-control study","volume":"98","author":"Tian","year":"2019","journal-title":"Medicine"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/sj.cdd.4401837","article-title":"Roles for NF-\u03baB in nerve cell survival, plasticity, and disease","volume":"13","author":"Mattson","year":"2006","journal-title":"Cell Death Differ."},{"doi-asserted-by":"crossref","unstructured":"Golding, A.P., Ferrier, B., New, L.A., Lu, P., Martin, C.E., Shata, E., Jones, R.A., Moorehead, R.A., and Jones, N. (2023). Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development. J. Mammary Gland. Biol. Neoplasia, 28.","key":"ref_28","DOI":"10.1007\/s10911-023-09541-1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1107\/S0907444913004770","article-title":"High-resolution crystal structure of the catalytic domain of human dual-specificity phosphatase 26","volume":"69","author":"Won","year":"2013","journal-title":"Acta Crystallogr. Sect. D Struct. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/onc.2008.431","article-title":"Protein phosphatase Dusp26 associates with KIF3 motor and promotes N-cadherin-mediated cell-cell adhesion","volume":"28","author":"Tanuma","year":"2009","journal-title":"Oncogene"},{"doi-asserted-by":"crossref","unstructured":"Hu, K., Guo, Y., Li, Y., Zhou, S., Lu, C., Cai, C., Yang, H., Li, Y., and Wang, W. (2023). Identification and Validation of PTGS2 Gene as an Oxidative Stress-Related Biomarker for Arteriovenous Fistula Failure. Antioxidants, 13.","key":"ref_31","DOI":"10.3390\/antiox13010005"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1152\/physrev.2001.81.2.741","article-title":"Alzheimer\u2019s disease: Genes, proteins, and therapy","volume":"81","author":"Selkoe","year":"2001","journal-title":"Physiol. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1126\/science.1132814","article-title":"A century of Alzheimer\u2019s disease","volume":"314","author":"Goedert","year":"2006","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.3233\/JAD-2006-9S335","article-title":"GSK-3 is essential in the pathogenesis of Alzheimer\u2019s disease","volume":"9","author":"Takashima","year":"2006","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.neurobiolaging.2013.08.032","article-title":"Interactions between GSK3\u03b2 and amyloid genes explain variance in amyloid burden","volume":"35","author":"Hohman","year":"2014","journal-title":"Neurobiol. Aging"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0092-8674(00)00116-1","article-title":"Signal transduction by the JNK group of MAP kinases","volume":"103","author":"Davis","year":"2000","journal-title":"Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17023","DOI":"10.1038\/sigtrans.2017.23","article-title":"NF-\u03baB signaling in inflammation","volume":"2","author":"Liu","year":"2017","journal-title":"Signal Transduct. Target. Ther."},{"doi-asserted-by":"crossref","unstructured":"Rex, J., Lutz, A., Faletti, L.E., Albrecht, U., Thomas, M., Bode, J.G., Borner, C., Sawodny, O., and Merfort, I. (2019). IL-1\u03b2 and TNF\u03b1 Differentially Influence NF-\u03baB Activity and FasL-Induced Apoptosis in Primary Murine Hepatocytes During LPS-Induced Inflammation. Front. Physiol., 10.","key":"ref_38","DOI":"10.3389\/fphys.2019.00117"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1007\/s00018-022-04162-z","article-title":"Dusp26 phosphatase regulates mitochondrial respiration and oxidative stress and protects neuronal cell death","volume":"79","author":"Eroglu","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.virusres.2018.05.028","article-title":"Role of MAPK\/MNK1 signaling in virus replication","volume":"253","author":"Kumar","year":"2018","journal-title":"Virus Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.4049\/jimmunol.1700373","article-title":"A Consensus Definitive Classification of Scavenger Receptors and Their Roles in Health and Disease","volume":"198","author":"PrabhuDas","year":"2017","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.leukres.2013.03.004","article-title":"Scavenger receptor class A member 3 (SCARA3) in disease progression and therapy resistance in multiple myeloma","volume":"37","author":"Brown","year":"2013","journal-title":"Leuk. Res."},{"doi-asserted-by":"crossref","unstructured":"Zub, K.A., de Sousa, M.M.L., Sarno, A., Sharma, A., Demirovic, A., Rao, S., Young, C., Aas, P.A., Ericsson, I., and Sundan, A. (2015). Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. PLoS ONE, 10.","key":"ref_43","DOI":"10.1371\/journal.pone.0119857"},{"doi-asserted-by":"crossref","unstructured":"Kim, J., You, H.J., and Youn, C. (2022). SCARA3 inhibits cell proliferation and EMT through AKT signaling pathway in lung cancer. BMC Cancer, 22.","key":"ref_44","DOI":"10.1186\/s12885-022-09631-z"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"10832","DOI":"10.1073\/pnas.93.20.10832","article-title":"Association of a novel human FE65-like protein with the cytoplasmic domain of the beta-amyloid precursor protein","volume":"93","author":"Chen","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1002\/jnr.21532","article-title":"The FE65 proteins and Alzheimer\u2019s disease","volume":"86","author":"McLoughlin","year":"2008","journal-title":"J. Neurosci. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"R605","DOI":"10.1186\/bcr920","article-title":"Role of Notch signaling in cell-fate determination of human mammary stem\/progenitor cells","volume":"6","author":"Dontu","year":"2004","journal-title":"Breast Cancer Res."},{"doi-asserted-by":"crossref","unstructured":"Xiong, J., Bao, J., Hu, W., Shang, M., and Zhang, L. (2023). Whole-genome resequencing reveals genetic diversity and selection characteristics of dairy goat. Front. Genet., 13.","key":"ref_48","DOI":"10.3389\/fgene.2022.1044017"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.smallrumres.2011.04.014","article-title":"Relationship between somatic cell count and bacterial pathogens in goat milk","volume":"100","author":"Bagnicka","year":"2011","journal-title":"Small Rumin. Res."},{"key":"ref_50","first-page":"59","article-title":"Structure and the non-genetic and genetic effects on milk traits in Polish dairy goat population","volume":"33","author":"Bagnicka","year":"2015","journal-title":"Anim. Sci. Pap. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1042\/BJ20082234","article-title":"Dual-specificity phosphatases: Critical regulators with diverse cellular targets","volume":"418","author":"Patterson","year":"2009","journal-title":"Biochem. J."},{"key":"ref_52","first-page":"39","article-title":"Analysis of the CAEV infection impact on the milk yield and milk SCC of polish dairy goats","volume":"3","author":"Bagnicka","year":"2014","journal-title":"J. Microbiol. Biotechnol. Food Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.4238\/2014.January.24.1","article-title":"Polymorphisms of PRLR and FOLR1 genes and association with milk production traits in goats","volume":"13","author":"Hou","year":"2014","journal-title":"Genet. Mol. Res."},{"doi-asserted-by":"crossref","unstructured":"Xuan, R., Wang, J., Zhao, X., Li, Q., Wang, Y., Du, S., Duan, Q., Guo, Y., Ji, Z., and Chao, T. (2022). Transcriptome Analysis of Goat Mammary Gland Tissue Reveals the Adaptive Strategies and Molecular Mechanisms of Lactation and Involution. Int. J. Mol. Sci., 23.","key":"ref_54","DOI":"10.3390\/ijms232214424"},{"doi-asserted-by":"crossref","unstructured":"Dige, M.S., Gurao, A., Singh, L.P., Chitkara, M., Singh, M.K., Dass, G., Verma, A.K., Pundir, R.K., and Kataria, R.S. (2024). Transcriptomic analysis reveals molecular insights into lactation dynamics in Jakhrana goat mammary gland. BMC Genom., 25.","key":"ref_55","DOI":"10.1186\/s12864-024-10744-x"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/7\/944\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:03:56Z","timestamp":1760033036000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/7\/944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":55,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["v17070944"],"URL":"https:\/\/doi.org\/10.3390\/v17070944","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2025,7,3]]}}}