{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:05:46Z","timestamp":1787630746754,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Stephen Babcock AG Chem Graduate Fellowship","award":["NIH\/NIAID AI158463"],"award-info":[{"award-number":["NIH\/NIAID AI158463"]}]},{"name":"Wisconsin Alumni Research Foundation","award":["NIH\/NIAID AI158463"],"award-info":[{"award-number":["NIH\/NIAID AI158463"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Feline coronavirus (FCoV), the causative agent behind feline infectious peritonitis (FIP), is one of the biggest infectious threats to feline health. Despite this threat, the tissue distribution and viral RNA levels in cats infected with feline coronaviruses are poorly understood in the context of natural infection. Here, we used a two-step reverse-transcription quantitative PCR (RT-qPCR) to examine viral RNA levels from different sampling sites in both cats that have been clinically suspected of FIP and their feline housemates. We show that the distribution and amount of FCoV viral RNA does not differ between FCoV-infected cats with FIP and their feline housemates in blood, conjunctiva, or feces. Furthermore, in all FIP and non-FIP cases, viral RNA levels were higher in fecal samples than the blood. Taken together, these results show that amount of viral RNA does not differ between FCoV-infected cats with FIP and their healthy housemates in several sample types. Our results indicate a need for closer examination of FCoV pathogenesis independent of viral dissemination, including an assessment of intrahost evolution of FCoVs and FCoVs\u2019 interactions with the feline immune system.<\/jats:p>","DOI":"10.3390\/v17070948","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T11:19:27Z","timestamp":1751887167000},"page":"948","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Feline Coronavirus RNA in Cats with Feline Infectious Peritonitis and Their Housemates"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4165-8952","authenticated-orcid":false,"given":"Phoenix M.","family":"Shepherd","sequence":"first","affiliation":[{"name":"Biochemistry Department, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Center for Quantitative Cell Imaging, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Elbe","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna M.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0949-5698","authenticated-orcid":false,"given":"Erin","family":"Lashnits","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5974-2709","authenticated-orcid":false,"given":"Robert N.","family":"Kirchdoerfer","sequence":"additional","affiliation":[{"name":"Biochemistry Department, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Center for Quantitative Cell Imaging, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/s41579-018-0118-9","article-title":"Origin and evolution of pathogenic coronaviruses","volume":"17","author":"Cui","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.tree.2005.02.009","article-title":"Emerging pathogens: The epidemiology and evolution of species jumps","volume":"20","author":"Woolhouse","year":"2005","journal-title":"Trends Ecol. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.onehlt.2016.12.001","article-title":"MERS-coronavirus: From discovery to intervention","volume":"3","author":"Widagdo","year":"2016","journal-title":"One Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9119","DOI":"10.1128\/JVI.01279-15","article-title":"Two mutations were critical for bat-to-human transmission of Middle East respiratory syndrome coronavirus","volume":"89","author":"Yang","year":"2015","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.tim.2013.05.005","article-title":"Interspecies transmission and emergence of novel viruses: Lessons from bats and birds","volume":"21","author":"Chan","year":"2013","journal-title":"Trends Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Domingo, E., Garc\u00eda-Crespo, C., Lobo-Vega, R., and Perales, C. (2021). Mutation Rates, Mutation Frequencies, and Proofreading-Repair Activities in RNA Virus Genetics. Viruses, 13.","DOI":"10.3390\/v13091882"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Flores-Vega, V.R., Monroy-Molina, J.V., Jim\u00e9nez-Hern\u00e1ndez, L.E., Torres, A.G., Santos-Preciado, J.I., and Rosales-Reyes, R. (2022). SARS-CoV-2: Evolution and Emergence of New Viral Variants. Viruses, 14.","DOI":"10.3390\/v14040653"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"ARohaim, M., FEl Naggar, R., MHelal, A., MBayoumi, M., AEl-Saied, M., AAhmed, K., ZShabbir, M., and Munir, M. (2019). Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds. Viruses, 11.","DOI":"10.3390\/v11010057"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/CMR.00102-14","article-title":"Middle East respiratory syndrome coronavirus: Another zoonotic betacoronavirus causing SARS-like disease","volume":"28","author":"Chan","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.vetmic.2003.07.010","article-title":"Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis","volume":"99","author":"Benetka","year":"2004","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1099\/vir.0.19129-0","article-title":"Persistence and transmission of natural type I feline coronavirus infection","volume":"84","author":"Addie","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_12","first-page":"1209","article-title":"Feline coronavirus serotypes 1 and 2: Seroprevalence and association with disease in Switzerland","volume":"12","author":"Kummrow","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/tbed.13081","article-title":"Circulation and genetic diversity of Feline coronavirus type I and II from clinically healthy and FIP-suspected cats in China","volume":"66","author":"Li","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"199059","DOI":"10.1016\/j.virusres.2023.199059","article-title":"An updated review of feline coronavirus: Mind the two biotypes","volume":"326","author":"Gao","year":"2023","journal-title":"Virus Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.tvjl.2014.04.017","article-title":"An update on feline infectious peritonitis: Virology and immunopathogenesis","volume":"201","author":"Pedersen","year":"2014","journal-title":"Vet. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1099\/vir.0.020214-0","article-title":"Sites of feline coronavirus persistence in healthy cats","volume":"91","author":"Kipar","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1051\/vetres\/2010043","article-title":"Pathogenic characteristics of persistent feline enteric coronavirus infection in cats","volume":"41","author":"Vogel","year":"2010","journal-title":"Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1177\/1098612X15574757","article-title":"Detection of feline coronavirus in cerebrospinal fluid for diagnosis of feline infectious peritonitis in cats with and without neurological signs","volume":"18","author":"Doenges","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"368","DOI":"10.2460\/ajvr.1981.42.03.368","article-title":"An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis","volume":"42","author":"Pedersen","year":"1981","journal-title":"Am. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.virusres.2011.12.020","article-title":"Feline infectious peritonitis: Role of the feline coronavirus 3c gene in intestinal tropism and pathogenicity based upon isolates from resident and adopted shelter cats","volume":"165","author":"Pedersen","year":"2012","journal-title":"Virus Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"14122","DOI":"10.1128\/JVI.79.22.14122-14130.2005","article-title":"Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein","volume":"79","author":"Rottier","year":"2005","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Felten, S., and Hartmann, K. (2019). Diagnosis of Feline Infectious Peritonitis: A Review of the Current Literature. Viruses, 11.","DOI":"10.3390\/v11111068"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.virol.2017.12.027","article-title":"Feline coronavirus: Insights into viral pathogenesis based on the spike protein structure and function","volume":"517","author":"Jaimes","year":"2018","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.3201\/eid1907.121094","article-title":"Mutation in spike protein cleavage site and pathogenesis of feline coronavirus","volume":"19","author":"Licitra","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Martinez, A., Lavergne, E., Brauge, C., Laveran, E., Bertagnoli, S., Boucraut-Baralon, C., and Bessi\u00e8re, P. (2025). Feline coronavirus-associated uveitis: The eye as a gateway to systemic spread and feline infectious peritonitis?. Vet. Microbiol., 301.","DOI":"10.1016\/j.vetmic.2024.110355"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Phyu, E.M., Charoenkul, K., Nasamran, C., Chamsai, E., Thaw, Y.N., Phyu, H.W., Soe, H.W., Chaiyawong, S., and Amonsin, A. (2025). Whole genome characterization of feline coronaviruses in Thailand: Evidence of genetic recombination and mutation M1058L in pathotype switch. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1451967"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Krentz, D., Zenger, K., Alberer, M., Felten, S., Bergmann, M., Dorsch, R., Matiasek, K., Kolberg, L., Hofmann-Lehmann, R., and Meli, M.L. (2021). Curing Cats with Feline Infectious Peritonitis with an Oral Multi-Component Drug Containing GS-441524. Viruses, 13.","DOI":"10.3390\/v13112228"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4942","DOI":"10.1128\/JVI.03688-14","article-title":"Broad-spectrum inhibitors against 3C-like proteases of feline coronaviruses and feline caliciviruses","volume":"89","author":"Kim","year":"2015","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.vetmic.2014.10.025","article-title":"Levels of feline infectious peritonitis virus in blood, effusions, and various tissues and the role of lymphopenia in disease outcome following experimental infection","volume":"175","author":"Pedersen","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1292\/jvms.18-0702","article-title":"Pathogenesis of oral type I feline infectious peritonitis virus (FIPV) infection: Antibody-dependent enhancement infection of cats with type I FIPV via the oral route","volume":"81","author":"Takano","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1292\/jvms.60.49","article-title":"Antibody-dependent enhancement of feline infectious peritonitis virus infection in feline alveolar macrophages and human monocyte cell line U937 by serum of cats experimentally or naturally infected with feline coronavirus","volume":"60","author":"Hohdatsu","year":"1998","journal-title":"J. Vet. Med. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1939-1676.1998.tb02144.x","article-title":"Diagnostic features of clinical neurologic feline infectious peritonitis","volume":"12","author":"Foley","year":"1998","journal-title":"J. Vet. Intern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.tvjl.2013.11.018","article-title":"Ocular manifestations of feline viral diseases","volume":"201","author":"Stiles","year":"2014","journal-title":"Vet. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1354\/vp.42-3-321","article-title":"Morphologic features and development of granulomatous vasculitis in feline infectious peritonitis","volume":"42","author":"Kipar","year":"2005","journal-title":"Vet. Pathol."},{"key":"ref_35","first-page":"1091","article-title":"Diagnosis and clinical signs of feline infectious peritonitis in the central nervous system","volume":"50","author":"Diaz","year":"2009","journal-title":"Can. Vet. J."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Attipa, C., Warr, A.S., Epaminondas, D., O\u2019Shea, M., Fletcher, S., Malbon, A., Lyraki, M., Hammond, R., Hardas, A., and Zanti, A. (2024). Emergence and spread of feline infectious peritonitis due to a highly pathogenic canine\/feline recombinant coronavirus. bioRxiv.","DOI":"10.1101\/2023.11.08.566182"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/s41467-020-20542-0","article-title":"Mechanism of SARS-CoV-2 polymerase stalling by remdesivir","volume":"12","author":"Kokic","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1111\/jvim.16804","article-title":"Thirty-two cats with effusive or non-effusive feline infectious peritonitis treated with a combination of remdesivir and GS-441524","volume":"37","author":"Green","year":"2023","journal-title":"J. Vet. Intern. Med."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Meli, M.L., Spiri, A.M., Zwicklbauer, K., Krentz, D., Felten, S., Bergmann, M., Dorsch, R., Matiasek, K., Alberer, M., and Kolberg, L. (2022). Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524. Viruses, 14.","DOI":"10.3390\/v14051069"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1177\/1098612X17729626","article-title":"Efficacy of a 3C-like protease inhibitor in treating various forms of acquired feline infectious peritonitis","volume":"20","author":"Pedersen","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kim, Y., Liu, H., Galasiti Kankanamalage, A.C., Weerasekara, S., Hua, D.H., Groutas, W.C., Chang, K.O., and Pedersen, N.C. (2016). Reversal of the Progression of Fatal Coronavirus Infection in Cats by a Broad-Spectrum Coronavirus Protease Inhibitor. PLoS Pathog., 12, Erratum in PLoS Pathog. 2016, 12, e1005650.","DOI":"10.1371\/journal.ppat.1005650"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12985-023-02273-y","article-title":"Prevalence of natural feline coronavirus infection in domestic cats in Fujian, China","volume":"21","author":"Dong","year":"2024","journal-title":"Virol. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1177\/1098612X15625354","article-title":"Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of feline infectious peritonitis","volume":"19","author":"Doenges","year":"2017","journal-title":"J. Feline Med. Surg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1128\/jcm.33.3.684-689.1995","article-title":"Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR","volume":"33","author":"Herrewegh","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1177\/1098612X17705227","article-title":"Cross-sectional quantitative RT-PCR study of feline coronavirus viremia and replication in peripheral blood of healthy shelter cats in Southern California","volume":"20","author":"Fish","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.rvsc.2017.10.004","article-title":"Discrepancies between feline coronavirus antibody and nucleic acid detection in effusions of cats with suspected feline infectious peritonitis","volume":"125","author":"Lorusso","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jfms.2007.10.010","article-title":"Evaluation of real-time RT-PCR for the quantification of FCoV shedding in the faeces of domestic cats","volume":"10","author":"Dye","year":"2008","journal-title":"J. Feline Med. Surg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1177\/1098612X221118761","article-title":"2022 AAFP\/EveryCat Feline Infectious Peritonitis Diagnosis Guidelines","volume":"24","author":"Thayer","year":"2022","journal-title":"J. Feline Med. Surg."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"W276","DOI":"10.1093\/nar\/gkac240","article-title":"Search and sequence analysis tools services from EMBL-EBI in 2022","volume":"50","author":"Madeira","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.jfms.2007.03.002","article-title":"The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR","volume":"9","author":"Pinar","year":"2007","journal-title":"J. Feline Med. Surg."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Nunes, B.T.D., de Mendon\u00e7a, M.H.R., Simith, D.B., Moraes, A.F., Cardoso, C.C., Prazeres, I.T.E., de Aquino, A.A., Santos, A.D.C.M., Queiroz, A.L.N., and Rodrigues, D.S.G. (2019). Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007884"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"113926","DOI":"10.1016\/j.jviromet.2020.113926","article-title":"Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay","volume":"284","author":"Hirotsu","year":"2020","journal-title":"J. Virol. Methods"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Bui, H.T.V., Bui, H.T., Chu, S.V., Nguyen, H.T., Nguyen, A.T.V., Truong, P.T., Dang, T.T.H., and Nguyen, A.T.V. (2023). Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0282439"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0378-1135(98)00210-7","article-title":"Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis","volume":"62","author":"Harbour","year":"1998","journal-title":"Vet. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1136\/vr.139.21.522","article-title":"Feline coronavirus in the intestinal contents of cats with feline infectious peritonitis","volume":"139","author":"Addie","year":"1996","journal-title":"Vet. Rec."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jfms.2003.08.007","article-title":"High viral loads despite absence of clinical and pathological findings in cats experimentally infected with feline coronavirus (FCoV) type I and in naturally FCoV-infected cats","volume":"6","author":"Meli","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Felten, S., Klein-Richers, U., Hofmann-Lehmann, R., Bergmann, M., Unterer, S., Leutenegger, C.M., and Hartmann, K. (2020). Correlation of Feline Coronavirus Shedding in Feces with Coronavirus Antibody Titer. Pathogens, 9.","DOI":"10.3390\/pathogens9080598"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.rvsc.2022.09.010","article-title":"Shedding persistency and intensity patterns of feline coronavirus (FCoV) in feces of cats living in breeding catteries in the Czech Republic","volume":"152","year":"2022","journal-title":"Res. Vet. Sci."},{"key":"ref_59","first-page":"609465","article-title":"Feline and canine coronaviruses: Common genetic and pathobiological features","volume":"2011","year":"2011","journal-title":"Adv. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"8669","DOI":"10.1128\/jvi.70.12.8669-8674.1996","article-title":"Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I","volume":"70","author":"Tresnan","year":"1996","journal-title":"J. Virol."},{"key":"ref_61","first-page":"592","article-title":"Experimental inoculation of cats with canine coronavirus and subsequent challenge with feline infectious peritonitis virus","volume":"34","author":"Barlough","year":"1984","journal-title":"Lab. Anim. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/978-1-4684-5823-7_66","article-title":"Canine coronavirus infection in cats; a possible role in feline infectious peritonitis","volume":"276","author":"McArdle","year":"1990","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Terada, Y., Matsui, N., Noguchi, K., Kuwata, R., Shimoda, H., Soma, T., Mochizuki, M., and Maeda, K. (2014). Emergence of pathogenic coronaviruses in cats by homologous recombination between feline and canine coronaviruses. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0106534"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"J\u00e4hne, S., Felten, S., Bergmann, M., Erber, K., Matiasek, K., Meli, M.L., Hofmann-Lehmann, R., and Hartmann, K. (2022). Detection of Feline Coronavirus Variants in Cats Without Feline Infectious Peritonitis. Viruses, 14.","DOI":"10.3390\/v14081671"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"167844","DOI":"10.1016\/j.scitotenv.2023.167844","article-title":"Development of highly sensitive one-step reverse transcription-quantitative PCR for SARS-CoV-2 detection in wastewater","volume":"907","author":"Raya","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"150244","DOI":"10.1016\/j.scitotenv.2021.150244","article-title":"Monitoring SARS-CoV-2 in sewage: Toward sentinels with analytical accuracy","volume":"804","author":"Orschler","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_67","first-page":"266","article-title":"Analysis of one-step and two-step real-time RT-PCR using SuperScript III","volume":"16","author":"Wacker","year":"2005","journal-title":"J. Biomol. Tech."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/7\/948\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:04:31Z","timestamp":1760033071000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/7\/948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,4]]},"references-count":67,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["v17070948"],"URL":"https:\/\/doi.org\/10.3390\/v17070948","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,4]]}}}