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These viruses hijack host cellular membranes and proteins to facilitate their replication, primarily through the formation of replication organelles (ROs). However, the precise regulatory mechanisms underlying RO formation remain poorly understood. To elucidate these mechanisms, we conducted mass spectrometry analyses, identifying interactions between the host protein DnaJ homolog subfamily C member 11 (DNAJC11) and the SARS-CoV-2 nonstructural protein 3 (NSP3) protein. Notably, results showed that DNAJC11 depletion reduces SARS-CoV-2 infection, indicating possible positive regulatory involvement. But the ectopic expression of DNAJC11 did not lead to marked alterations in immune or inflammatory responses. DNAJC11 enhanced NSP3 expression stability through endogenous apoptosis pathways and facilitated its interaction with NSP4, thereby promoting the formation of double-membrane vesicles (DMVs). Knockdown of DNAJC11 reduced DMV number and size, accompanied by dysregulation of the endoplasmic reticulum and mitochondria. However, supplementation with DNAJC11 restored both DMV number and size. These findings provide novel insights into the role of DNAJC11 as a host factor that modulates DMV formation and supports SARS-CoV-2 replication by targeting the NSP3 protein. This study advances our understanding of the molecular interactions between host and viral components and highlights DNAJC11 as a potential target for antiviral interventions.<\/jats:p>","DOI":"10.3390\/v17081025","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:44:45Z","timestamp":1753184685000},"page":"1025","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["DNAJ Homolog Subfamily C Member 11 Stabilizes SARS-CoV-2 NSP3 to Promote Double-Membrane Vesicle Formation"],"prefix":"10.3390","volume":"17","author":[{"given":"Shuying","family":"Chen","sequence":"first","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanrong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoning","family":"Li","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Xiang","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangyu","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaokai","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Li","sequence":"additional","affiliation":[{"name":"Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Zang","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Henan Normal University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2600-6059","authenticated-orcid":false,"given":"Jian","family":"Shang","sequence":"additional","affiliation":[{"name":"Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushun","family":"Wan","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1038\/s41564-021-00958-0","article-title":"Cellular host factors for SARS-CoV-2 infection","volume":"6","author":"Baggen","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"104502","DOI":"10.1016\/j.meegid.2020.104502","article-title":"From SARS to SARS-CoV-2, insights on structure, pathogenicity and immunity aspects of pandemic human coronaviruses","volume":"85","author":"Kirtipal","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"12910","DOI":"10.1074\/jbc.REV120.013930","article-title":"The molecular virology of coronaviruses","volume":"295","author":"Hartenian","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S0140-6736(20)30251-8","article-title":"Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding","volume":"395","author":"Lu","year":"2020","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1099\/vir.0.052910-0","article-title":"MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-alpha treatment","volume":"94","author":"Raj","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mBio.00166-11","article-title":"The transformation of enterovirus replication structures: A three-dimensional study of single- and double-membrane compartments","volume":"2","author":"Limpens","year":"2011","journal-title":"mBio"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1128\/JVI.06677-11","article-title":"Ultrastructural characterization of arterivirus replication structures: Reshaping the endoplasmic reticulum to accommodate viral RNA synthesis","volume":"86","author":"Knoops","year":"2012","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e3000715","DOI":"10.1371\/journal.pbio.3000715","article-title":"A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis","volume":"18","author":"Snijder","year":"2020","journal-title":"PLoS Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1007\/s00415-020-10197-8","article-title":"Why do SARS-CoV-2 NSPs rush to the ER?","volume":"268","author":"Santerre","year":"2021","journal-title":"J. Neurol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1038\/s41586-022-04835-6","article-title":"The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle","volume":"606","author":"Ricciardi","year":"2022","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7894","DOI":"10.1038\/s41467-023-43666-5","article-title":"SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle","volume":"14","author":"Zimmermann","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1021\/acs.jproteome.9b00338","article-title":"Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice","volume":"18","author":"Violitzi","year":"2019","journal-title":"J. Proteome Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1074\/jbc.REV118.002810","article-title":"Recent advances in the structural and mechanistic aspects of Hsp70 molecular chaperones","volume":"294","author":"Mayer","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20160534","DOI":"10.1098\/rstb.2016.0534","article-title":"DNAJ Proteins in neurodegeneration: Essential and protective factors","volume":"373","author":"Zarouchlioti","year":"2018","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e4257","DOI":"10.21769\/BioProtoc.4257","article-title":"A Nucleocapsid-based Transcomplementation Cell Culture System of SARS-CoV-2 to Recapitulate the Complete Viral Life Cycle","volume":"11","author":"Yu","year":"2021","journal-title":"Bio. Protoc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/s41586-021-03493-4","article-title":"Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV","volume":"594","author":"Stukalov","year":"2021","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"eabe9403","DOI":"10.1126\/science.abe9403","article-title":"Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms","volume":"370","author":"Gordon","year":"2020","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e0050723","DOI":"10.1128\/jvi.00507-23","article-title":"The REEP5\/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication","volume":"97","author":"Li","year":"2023","journal-title":"J. Virol."},{"key":"ref_19","first-page":"104811","article-title":"Potent Antiviral Activities of Type I Interferons to SARS-CoV-2 Infection","volume":"179","author":"Mantlo","year":"2020","journal-title":"bioRxiv"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"114316","DOI":"10.1016\/j.bcp.2020.114316","article-title":"Detection of Viral Infections by Innate Immunity","volume":"183","author":"Carty","year":"2021","journal-title":"Biochem. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e104237","DOI":"10.1371\/journal.pone.0104237","article-title":"A splicing mutation in the novel mitochondrial protein DNAJC11 causes motor neuron pathology associated with cristae disorganization, and lymphoid abnormalities in mice","volume":"9","author":"Ioakeimidis","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e2213783119","DOI":"10.1073\/pnas.2213783119","article-title":"Recent common human coronavirus infection protects against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection: A Veterans Affairs cohort study","volume":"119","author":"Fillmore","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12250-016-3746-0","article-title":"Coronavirus: Epidemiology, genome replication and the interactions with their hosts","volume":"31","author":"Shi","year":"2016","journal-title":"Virol. Sin."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.coviro.2014.05.001","article-title":"The art of war: Battles between virus and host","volume":"6","author":"Frieman","year":"2014","journal-title":"Curr. Opin. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e202309140","DOI":"10.1083\/jcb.202309140","article-title":"LLPS of FXR proteins drives replication organelle clustering for beta-coronaviral proliferation","volume":"223","author":"Li","year":"2024","journal-title":"J. Cell Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1038\/s44319-023-00043-z","article-title":"The NSP3 protein of SARS-CoV-2 binds fragile X mental retardation proteins to disrupt UBAP2L interactions","volume":"25","author":"Garvanska","year":"2024","journal-title":"EMBO Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e202306020","DOI":"10.1083\/jcb.202306020","article-title":"RTN3 and RTN4: Architects of SARS-CoV-2 replication organelles","volume":"222","author":"Kratzel","year":"2023","journal-title":"J. Cell Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3545","DOI":"10.1016\/j.febslet.2007.06.052","article-title":"The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11","volume":"581","author":"Xie","year":"2007","journal-title":"FEBS Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1002\/pro.3725","article-title":"Structural and functional analysis of the Hsp70\/Hsp40 chaperone system","volume":"29","author":"Liu","year":"2020","journal-title":"Protein Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.virusres.2011.06.013","article-title":"Heat shock protein 40 (Hsp40) plays a key role in the virus life cycle","volume":"160","author":"Knox","year":"2011","journal-title":"Virus Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cao, Y.Q., Yuan, L., Zhao, Q., Yuan, J.L., Miao, C., Chang, Y.F., Wen, X.T., Wu, R., Huang, X.B., and Wen, Y.P. (2019). Hsp40 Protein DNAJB6 Interacts with Viral NS3 and Inhibits the Replication of the Japanese Encephalitis Virus. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20225719"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"19063","DOI":"10.1038\/srep19063","article-title":"Human Heat shock protein 40 (Hsp40\/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins","volume":"6","author":"Batra","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e0080722","DOI":"10.1128\/jvi.00807-22","article-title":"Hsp70 Inhibits the Replication of Fowl Adenovirus Serotype 4 by Suppressing Viral Hexon with the Assistance of DnaJC7","volume":"96","author":"Cao","year":"2022","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s11427-021-1964-4","article-title":"Overview of SARS-CoV-2 genome-encoded proteins","volume":"65","author":"Bai","year":"2022","journal-title":"Sci. China Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"166725","DOI":"10.1016\/j.jmb.2020.11.024","article-title":"Structural insights into SARS-CoV-2 proteins","volume":"433","author":"Arya","year":"2021","journal-title":"J. Mol. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1080\/15548627.2022.2103783","article-title":"DMV biogenesis during beta-coronavirus infection requires autophagy proteins VMP1 and TMEM41B","volume":"19","author":"Ji","year":"2023","journal-title":"Autophagy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/s41586-024-07817-y","article-title":"Molecular architecture of coronavirus double-membrane vesicle pore complex","volume":"633","author":"Huang","year":"2024","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/cmi.12372","article-title":"Virus-induced double-membrane vesicles","volume":"17","author":"Blanchard","year":"2015","journal-title":"Cell Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"111774","DOI":"10.1016\/j.celrep.2022.111774","article-title":"Mitochondrial cristae architecture protects against mtDNA release and inflammation","volume":"41","author":"He","year":"2022","journal-title":"Cell Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"738794","DOI":"10.1155\/2013\/738794","article-title":"Viruses as modulators of mitochondrial functions","volume":"2013","author":"Anand","year":"2013","journal-title":"Adv. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"110049","DOI":"10.1016\/j.celrep.2021.110049","article-title":"Contribution of autophagy machinery factors to HCV and SARS-CoV-2 replication organelle formation","volume":"37","author":"Twu","year":"2021","journal-title":"Cell Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00018-022-04469-x","article-title":"The double-membrane vesicle (DMV): A virus-induced organelle dedicated to the replication of SARS-CoV-2 and other positive-sense single-stranded RNA viruses","volume":"79","author":"Roingeard","year":"2022","journal-title":"Cell. Mol. Life. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s00203-020-01998-6","article-title":"SARS-CoV-2 nucleocapsid and Nsp3 binding: An in silico study","volume":"203","author":"Khan","year":"2021","journal-title":"Arch. Microbiol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/8\/1025\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:13:54Z","timestamp":1760033634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/8\/1025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,22]]},"references-count":43,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["v17081025"],"URL":"https:\/\/doi.org\/10.3390\/v17081025","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,22]]}}}