{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:37Z","timestamp":1792790197836,"version":"4.5.0"},"reference-count":137,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Infrastructure Program","award":["NSTI-BMCR23"],"award-info":[{"award-number":["NSTI-BMCR23"]}]},{"name":"National Science and Technology Infrastructure Program","award":["NSTI-BMCR24"],"award-info":[{"award-number":["NSTI-BMCR24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Epstein\u2013Barr virus (EBV) is a prevalent virus linked to various diseases, including infectious mononucleosis (IM), nasopharyngeal carcinoma, and Hodgkin\u2019s lymphoma. Over the past few decades, EBV diagnostic strategies have evolved significantly\u2014progressing from traditional serological assays and histopathology to more sensitive and specific molecular techniques such as nucleic acid amplification and high-throughput sequencing (HTS). While conventional methods remain valuable for their accessibility and established clinical use, they are often limited by sensitivity, speed, and multiplexing capability. In contrast, emerging technologies, including isothermal amplification, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based diagnostics, multi-omics integration, and AI-assisted analysis, have demonstrated great promise in improving diagnostic accuracy, speed, and applicability in diverse clinical settings, including point-of-care testing (POCT). This review systematically explores the historical development of EBV diagnostic technologies, highlighting key milestones and future trends in precision medicine and global health readiness.<\/jats:p>","DOI":"10.3390\/v17081026","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:44:45Z","timestamp":1753184685000},"page":"1026","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Advances in Epstein\u2013Barr Virus Detection: From Traditional Methods to Modern Technologies"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5588-3772","authenticated-orcid":false,"given":"Yidan","family":"Sun","sequence":"first","affiliation":[{"name":"China Center for Type Culture Collection, Wuhan University, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Ling","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Wuhan University, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dani","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Wuhan University, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2498-2769","authenticated-orcid":false,"given":"Meimei","family":"Yang","sequence":"additional","affiliation":[{"name":"China Center for Type Culture Collection, Wuhan University, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4687-0127","authenticated-orcid":false,"given":"Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"China Center for Type Culture Collection, Wuhan University, Wuhan 430072, China"},{"name":"College of Life Sciences, Wuhan University, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.coi.2020.01.004","article-title":"Human inborn errors of immunity to herpes viruses","volume":"62","author":"Jouanguy","year":"2020","journal-title":"Curr. 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