{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:00:05Z","timestamp":1791932405259,"version":"4.3.4"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology of China","award":["2022YFD1800500"],"award-info":[{"award-number":["2022YFD1800500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Nipah virus (NiV) is a highly pathogenic bat-borne zoonotic pathogen that poses a significant threat to human and animal health, with fatality rates exceeding 70% in some outbreaks. Despite its significant public health impact, there are currently no licensed vaccines or specific therapeutics available. Various virological tools\u2014such as reverse genetics systems, replicon particles, VSV-based pseudoviruses, and recombinant Cedar virus chimeras\u2014have been widely used to study the molecular mechanisms of NiV and to support vaccine development. Building upon these platforms, we developed a replication-competent recombinant vesicular stomatitis virus (rVSV\u0394G-eGFP-NiVBD F\/G) expressing NiV attachment (G) and fusion (F) glycoproteins. This recombinant virus serves as a valuable tool for investigating NiV entry mechanisms, cellular tropism, and immunogenicity. The virus was generated by replacing the VSV G protein with NiV F\/G through reverse genetics, and protein incorporation was confirmed via immunofluorescence and electron microscopy. In vitro, the virus exhibited robust replication, characteristic cell tropism, and high viral titers in multiple cell lines. Neutralization assays showed that monoclonal antibodies HENV-26 and HENV-32 effectively neutralized the recombinant virus. Furthermore, immunization of golden hamsters with inactivated rVSV\u0394G-eGFP-NiVBD F\/G induced potent neutralizing antibody responses, demonstrating its robust immunogenicity. These findings highlight rVSV\u0394G-eGFP-NiVBD F\/G as an effective platform for NiV research and vaccine development.<\/jats:p>","DOI":"10.3390\/v17081070","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T06:37:47Z","timestamp":1754030267000},"page":"1070","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and Immunogenic Evaluation of a Recombinant Vesicular Stomatitis Virus Expressing Nipah Virus F and G Glycoproteins"],"prefix":"10.3390","volume":"17","author":[{"given":"Huijuan","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijing","family":"Dang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8276-6767","authenticated-orcid":false,"given":"Shuhuai","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Shan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xijun","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinying","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigao","family":"Bu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3112-0243","authenticated-orcid":false,"given":"Zhiyuan","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1126\/science.288.5470.1432","article-title":"Nipah virus: A recently emergent deadly paramyxovirus","volume":"288","author":"Chua","year":"2000","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.3201\/eid1307.061128","article-title":"Person-to-person transmission of Nipah virus in a Bangladeshi community","volume":"13","author":"Gurley","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/S0140-6736(99)04299-3","article-title":"Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia","volume":"354","author":"Chua","year":"1999","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1046\/j.1365-2990.2000.00256.x","article-title":"Emerging and re-emerging epidemic encephalitis: A tale of two viruses","volume":"26","author":"Wong","year":"2000","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1016\/S0002-9440(10)64493-8","article-title":"Nipah virus infection: Pathology and pathogenesis of an emerging paramyxoviral zoonosis","volume":"161","author":"Wong","year":"2002","journal-title":"Am. J. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Liew, Y.J.M., Ibrahim, P.A.S., Ong, H.M., Chong, C.N., Tan, C.T., Schee, J.P., G\u00f3mez Rom\u00e1n, R., Cherian, N.G., Wong, W.F., and Chang, L.Y. (2022). The Immunobiology of Nipah Virus. Microorganisms, 10.","DOI":"10.3390\/microorganisms10061162"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e70195","DOI":"10.1002\/hsr2.70195","article-title":"Nipah Virus Outbreaks in Kerala: An Impending Doom?","volume":"7","author":"Sanker","year":"2024","journal-title":"Health Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100434","DOI":"10.1016\/j.ijregi.2024.100434","article-title":"Twenty-five years of Nipah outbreaks in Southeast Asia: A persistent threat to global health","volume":"13","author":"Khan","year":"2024","journal-title":"IJID Reg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.coviro.2012.02.016","article-title":"Henipavirus outbreaks to antivirals: The current status of potential therapeutics","volume":"2","author":"Broder","year":"2012","journal-title":"Curr. Opin. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bruno, L., Nappo, M.A., Ferrari, L., Di Lecce, R., Guarnieri, C., Cantoni, A.M., and Corradi, A. (2022). Nipah Virus Disease: Epidemiological, Clinical, Diagnostic and Legislative Aspects of This Unpredictable Emerging Zoonosis. Animals, 13.","DOI":"10.3390\/ani13010159"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.virol.2007.11.011","article-title":"Functional studies of host-specific ephrin-B ligands as Henipavirus receptors","volume":"372","author":"Bossart","year":"2008","journal-title":"Virology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/nrmicro1323","article-title":"Hendra and Nipah viruses: Different and dangerous","volume":"4","author":"Eaton","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gazal, S., Sharma, N., Gazal, S., Tikoo, M., Shikha, D., Badroo, G.A., Rashid, M., and Lee, S.J. (2022). Nipah and Hendra Viruses: Deadly Zoonotic Paramyxoviruses with the Potential to Cause the Next Pandemic. Pathogens, 11.","DOI":"10.3390\/pathogens11121419"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2224642","DOI":"10.1080\/21505594.2023.2224642","article-title":"Genomic characterization, transcriptome analysis, and pathogenicity of the Nipah virus (Indian isolate)","volume":"14","author":"Mohandas","year":"2023","journal-title":"Virulence"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"280","DOI":"10.3390\/v4020280","article-title":"Henipavirus mediated membrane fusion, virus entry and targeted therapeutics","volume":"4","author":"Steffen","year":"2012","journal-title":"Viruses"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jviromet.2007.01.003","article-title":"Neutralization assays for differential henipavirus serology using Bio-Plex protein array systems","volume":"142","author":"Bossart","year":"2007","journal-title":"J. Virol. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bossart, K.N., Zhu, Z., Middleton, D., Klippel, J., Crameri, G., Bingham, J., McEachern, J.A., Green, D., Hancock, T.J., and Chan, Y.P. (2009). A neutralizing human monoclonal antibody protects against lethal disease in a new ferret model of acute nipah virus infection. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000642"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"eadh4057","DOI":"10.1126\/sciadv.adh4057","article-title":"Single-dose mucosal replicon-particle vaccine protects against lethal Nipah virus infection up to 3 days after vaccination","volume":"9","author":"Welch","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106013","DOI":"10.1016\/j.antiviral.2024.106013","article-title":"Optimization of Bangladesh and Malaysian genotype recombinant reporter Nipah viruses for in vitro antiviral screening and in vivo disease modeling","volume":"231","author":"Lo","year":"2024","journal-title":"Antivir. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Amaya, M., Yin, R., Yan, L., Borisevich, V., Adhikari, B.N., Bennett, A., Malagon, F., Cer, R.Z., Bishop-Lilly, K.A., and Dimitrov, A.S. (2023). A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses. Viruses, 15.","DOI":"10.3390\/v15051077"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jviromet.2009.04.037","article-title":"A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein","volume":"160","author":"Kaku","year":"2009","journal-title":"J. Virol. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e0195723","DOI":"10.1128\/jvi.01957-23","article-title":"Establishment of replication-competent vesicular stomatitis virus recapitulating SADS-CoV entry","volume":"98","author":"Zhu","year":"2024","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5458","DOI":"10.1128\/JVI.78.10.5458-5465.2004","article-title":"Properties of replication-competent vesicular stomatitis virus vectors expressing glycoproteins of filoviruses and arenaviruses","volume":"78","author":"Garbutt","year":"2004","journal-title":"J. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"O\u2019Donnell, K.L., Feldmann, F., Kaza, B., Clancy, C.S., Hanley, P.W., Fletcher, P., and Marzi, A. (2023). Rapid protection of nonhuman primates against Marburg virus disease using a single low-dose VSV-based vaccine. EBioMedicine, 89.","DOI":"10.1016\/j.ebiom.2023.104463"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.antiviral.2017.12.007","article-title":"A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization","volume":"150","author":"Liu","year":"2018","journal-title":"Antivir. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1056\/NEJMoa1414216","article-title":"A Recombinant Vesicular Stomatitis Virus Ebola Vaccine","volume":"376","author":"Regules","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.antiviral.2013.10.012","article-title":"Single-dose replication-defective VSV-based Nipah virus vaccines provide protection from lethal challenge in Syrian hamsters","volume":"101","author":"Lo","year":"2014","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1186\/1743-422X-10-353","article-title":"Single injection recombinant vesicular stomatitis virus vaccines protect ferrets against lethal Nipah virus disease","volume":"10","author":"Mire","year":"2013","journal-title":"Virol. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.1016\/j.vaccine.2014.02.087","article-title":"Single-dose live-attenuated Nipah virus vaccines confer complete protection by eliciting antibodies directed against surface glycoproteins","volume":"32","author":"DeBuysscher","year":"2014","journal-title":"Vaccine"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1016\/j.vaccine.2015.03.089","article-title":"Single-dose live-attenuated vesicular stomatitis virus-based vaccine protects African green monkeys from Nipah virus disease","volume":"33","author":"Prescott","year":"2015","journal-title":"Vaccine"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.3201\/eid2506.181620","article-title":"Use of Single-Injection Recombinant Vesicular Stomatitis Virus Vaccine to Protect Nonhuman Primates Against Lethal Nipah Virus Disease","volume":"25","author":"Mire","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1016\/j.cell.2020.11.023","article-title":"Potent Henipavirus Neutralization by Antibodies Recognizing Diverse Sites on Hendra and Nipah Virus Receptor Binding Protein","volume":"183","author":"Dong","year":"2020","journal-title":"Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2449083","DOI":"10.1080\/22221751.2024.2449083","article-title":"A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease","volume":"14","author":"Yang","year":"2025","journal-title":"Emerg. Microbes Infect."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.virs.2024.03.010","article-title":"Establishment and application of a surrogate model for human Ebola virus disease in BSL-2 laboratory","volume":"39","author":"Yang","year":"2024","journal-title":"Virol. Sin."},{"key":"ref_35","first-page":"10","article-title":"Chikungunya, Influenza, Nipah, and Semliki Forest Chimeric Viruses with Vesicular Stomatitis Virus: Actions in the Brain","volume":"91","author":"Mao","year":"2017","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.virol.2012.06.001","article-title":"Newcastle disease virus-vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs","volume":"432","author":"Kong","year":"2012","journal-title":"Virology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10652","DOI":"10.1073\/pnas.0504887102","article-title":"Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus","volume":"102","author":"Bonaparte","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/nature03838","article-title":"EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus","volume":"436","author":"Negrete","year":"2005","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Negrete, O.A., Wolf, M.C., Aguilar, H.C., Enterlein, S., Wang, W., M\u00fchlberger, E., Su, S.V., Bertolotti-Ciarlet, A., Flick, R., and Lee, B. (2006). Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus. PLoS Pathog., 2.","DOI":"10.1371\/journal.ppat.0020007"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/8\/1070\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:19:43Z","timestamp":1760033983000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/8\/1070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":39,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["v17081070"],"URL":"https:\/\/doi.org\/10.3390\/v17081070","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,31]]}}}