{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:41:00Z","timestamp":1788993660118,"version":"build-2803163510"},"reference-count":67,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Assignment of The Ministry of Health of the Russian Federation","award":["121102500071-6"],"award-info":[{"award-number":["121102500071-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Despite the widespread accessibility of vaccines and antivirals, seasonal influenza virus epidemics continue to pose a threat to public health. In this study, we constructed a recombinant replication-deficient simian adenovirus type 25 vector carrying the full-length hemagglutinin (HA) of the H1N1 influenza virus, named rSAd25-H1. Both systemic and mucosal humoral immune responses, as well as the protective efficacy, were assessed in mice immunized via the intramuscular (IM) or intranasal (IN) route. A single-dose IM or IN administration of rSAd25-H1 elicited a robust systemic IgG antibody response, including hemagglutination inhibition antibodies. As expected, only IN immunization was able to induce IgA production in serum and respiratory mucosa. Notably, a single dose of rSAd25-H1 at the highest dose (1010 viral particles) conferred complete protection against lethal homologous H1N1 challenge in mice despite the route of administration. These findings demonstrate the potential of simian adenovirus type 25-based vectors as a promising candidate for intranasal vaccine development targeting respiratory pathogens.<\/jats:p>","DOI":"10.3390\/v17081085","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T13:44:32Z","timestamp":1754401472000},"page":"1085","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6629-744X","authenticated-orcid":false,"given":"Daria V.","family":"Voronina","sequence":"first","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina V.","family":"Vavilova","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Zubkova","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7147-1553","authenticated-orcid":false,"given":"Tatiana A.","family":"Ozharovskaia","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3248-1227","authenticated-orcid":false,"given":"Olga","family":"Popova","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia S.","family":"Chugunova","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1965-0482","authenticated-orcid":false,"given":"Polina P.","family":"Goldovskaya","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis I.","family":"Zrelkin","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2228-3406","authenticated-orcid":false,"given":"Daria M.","family":"Savina","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0639-8390","authenticated-orcid":false,"given":"Irina A.","family":"Favorskaya","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1289-3411","authenticated-orcid":false,"given":"Dmitry V.","family":"Shcheblyakov","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis Y.","family":"Logunov","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandr L.","family":"Gintsburg","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/S0140-6736(17)33293-2","article-title":"Estimates of Global Seasonal Influenza-Associated Respiratory Mortality: A Modelling Study","volume":"391","author":"Iuliano","year":"2018","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100851","DOI":"10.1016\/S2666-5247(24)00066-1","article-title":"Probable Extinction of Influenza B\/Yamagata and Its Public Health Implications: A Systematic Literature Review and Assessment of Global Surveillance Databases","volume":"5","author":"Caini","year":"2024","journal-title":"Lancet Microbe"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.vaccine.2021.01.032","article-title":"Variable Seasonal Influenza Vaccine Effectiveness across Geographical Regions, Age Groups and Levels of Vaccine Antigenic Similarity with Circulating Virus Strains: A Systematic Review and Meta-Analysis of the Evidence from Test-Negative Design Studies after the 2009\/10 Influenza Pandemic","volume":"39","author":"Okoli","year":"2021","journal-title":"Vaccine"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6047","DOI":"10.1016\/j.vaccine.2020.06.021","article-title":"The Impact of Candidate Influenza Virus and Egg-Based Manufacture on Vaccine Effectiveness: Literature Review and Expert Consensus","volume":"38","author":"Rajaram","year":"2020","journal-title":"Vaccine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e01693-18","DOI":"10.1128\/JVI.01693-18","article-title":"N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing","volume":"93","author":"An","year":"2019","journal-title":"J. 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