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Chinese Academy of Sciences","award":["XDB0490000"],"award-info":[{"award-number":["XDB0490000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Hepatitis B virus (HBV) remains a major global health challenge, with over 296 million people chronically infected worldwide. Despite the availability of antiviral therapies, a functional cure is rarely achieved, highlighting the need for novel therapeutic strategies. RNA 5-methylcytosine (m5C) is a pivotal epitranscriptomic mark implicated in RNA stability, transport, and translation. Emerging evidence shows that m5C is conserved within HBV RNA and plays critical roles in the viral life cycle. This review provides a comprehensive overview of the molecular mechanisms governing m5C deposition and recognition, summarizes recent advances in m5C biology, and highlights the emerging role of epitranscriptomic m5C regulation in HBV infection. We discuss the identification of HBV-specific m5C sites, the functions of key regulatory enzymes, and their interplay in viral RNA stabilization and evasion of innate immune responses. Interplay between m5C and other RNA modifications\u2014particularly N6-methyladenosine (m6A)\u2014is examined alongside virus-specific m5C regulation in EV71, HIV, HCV, EBV, and SARS-CoV-2. Potential links between m5C dysregulation and HBV-induced hepatocarcinogenesis are outlined, and emerging therapeutic strategies targeting the m5C machinery are highlighted. Together, these insights position the epitranscriptomic landscape as a promising avenue for innovative antiviral strategies.<\/jats:p>","DOI":"10.3390\/v17091159","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:09:32Z","timestamp":1756080572000},"page":"1159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Epitranscriptomic Regulation of Hepatitis B Virus by RNA 5-Methylcytosine: Functions, Mechanisms, and Therapeutic Potential"],"prefix":"10.3390","volume":"17","author":[{"given":"Xuliu","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuxiang","family":"Guan","sequence":"additional","affiliation":[{"name":"Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei JiangXia Laboratory, Wuhan 430200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Hao","sequence":"additional","affiliation":[{"name":"Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/apt.16828","article-title":"Review article: Hepatitis B-current and emerging therapies","volume":"55","author":"Yardeni","year":"2022","journal-title":"Aliment. 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